Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effect of Water Extract of Aloe in RANKL-induced Osteoclast Differentiation

파골세포 분화에 미치는 노회(蘆會) 추출물의 효과

  • Lee, Jeong-Hugh (Department of Anatomy, College of Medicine, Wonkwang University) ;
  • Lee, Myeung-Su (Department of Internal Medicine, Division of Rheumatology and Institute of Wonkwang Medical Science) ;
  • Chae, Soo-Uk (Department of Orthopedic Surgery, Gunsan Medical Center) ;
  • Kim, Ha-Young (Department of Internal Medicine, Division of Endocrinology and Metabolism, Sanbon Medical Center) ;
  • Moon, Seo-Young (Division of Anesthesiology and Pain Medicine, Sanbon Medical Center) ;
  • Jeon, Byung-Hoon (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Cho, Hae-Joong (Department of Obstetrics and Gynecology, College of Medicine, Wonkwang University)
  • 이정휴 (원광대학교 의과대학 해부학교실) ;
  • 이명수 (류마티스내과학교실 및 원광의과학 연구소) ;
  • 채수욱 (군산병원 정형외과학교실) ;
  • 김하영 (산본병원 내분비내과학교실) ;
  • 문서영 (산본병원 마취통증의학교실) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실) ;
  • 조해중 (원광대학교 의과대학 산부인과학교실)
  • Received : 2011.11.09
  • Accepted : 2011.12.12
  • Published : 2011.12.25
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Abstract

Osteoporosis is the leading underlying cause of fractures, particularly in postmenopausal women, due to the loss of estrogen-mediated suppression of bone resorption. More than 50% of adults 50 years of age or older are estimated to have osteoporosis. Osteoclast which is main target for treatment of osteoporosis is originated from hematopoietic cell line. Aloe has been widely used in worldwide country as a coadjuvant medicine. Extracts of the leaves of Aloe have been used in condition to improve dermatologic problem such as seborrheic dermatitis, aphthous stomatitis, xerosis, lichen planus and has been known to exert anti-inflammatory, anti-oxidant and anti-tumor effects. However, despite the popularity of aloe as a plant food supplements, the evaluation of its efficacy as a possible therapeutic option for osteoporosis remains scarce. Thus, we evaluated the effect of Aloe on receptor activator of nuclear factor-${\kappa}B$ ligand (RANKL)-induced osteoclast differentiation. Here we found that Aloe significantly inhibited osteoclast differentiation induced by RANKL. Aloe suppressed the activation of p38 pathway and $NF{\kappa}B$ in bone marrow macrophages (BMMs) treated with RANKL. Also, Aloe significantly inhibited the mRNA expression of c-Fos, tartrate-resistant acid phosphatase (TRAP), osteoclast-associated receptor (OSCAR), nuclear factor of activated T cells (NFAT)c1 and cathepsin K in BMMs treated with RANKL. Particularly, Aloe greatly inhibited the protein expression of c-fos and NFATc1. Taken together, our results suggested that Aloe may be useful tool for treatment of osteoporosis by inhibition of osteoclast differentiation.

Keywords

References

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