Experimental Studies on the Expression of Hair Growth Related Factors after Acupuncture & Moxibustion Therapy

침구요법(鍼灸療法)에 의한 발모관련 인자들의 발현에 대한 실험적 연구

  • Kim, Ho-Il (Department of Anatomy, College of Oriental Medicine, Woosuk University) ;
  • Kim, Cheong-Moo (Department of Anatomy, College of Oriental Medicine, Woosuk University) ;
  • Lee, Chang-Hyun (Department of Anatomy, College of Oriental Medicine, Woosuk University)
  • 김호일 (우석대학교 한의과대학 해부학교실) ;
  • 김정무 (우석대학교 한의과대학 해부학교실) ;
  • 이창현 (우석대학교 한의과대학 해부학교실)
  • Received : 2011.06.07
  • Accepted : 2011.08.12
  • Published : 2011.08.25

Abstract

The present study was undertaken to investigate the effect of acupuncture & moxibustion therapy on the hair follicle growth of skin 5 days and 10 days by macroscopic, microscopic and immunohistochemical methods. The results were as follows : Macroscopic hair follicle growth of plum-blossom needle treated group and strong moxibustion treated group was more increase than that of control group. Microscopic hair follicle growth of plum-blossom needle treated group and strong moxibustion treated group was hair growing cycle, anagen phase VI and that of control group and weak moxibustion treated group was hair growing cycle, anagen phase IV. Immunohistochemical observations on the expression of various growth factors, enzyme and receptor in hair follicle cycle after local treatment of acupuncture & moxibustion therapy are as follows: Expression of fibroblast growth factor was more intense in epidermis in plum-blossom needle treated group, epidermis and secondary hair germ cells in strong moxibustion treated group than control group. Expression of epidermal growth factor was more intense in epidermis in all experimental groups, and secondary hair germ cells in moxibustion treated group than control group. Expression of c-kit receptor was more intense in epidermis, secondary hair germ cells, outer root sheath in all experimental groups than control group. Expression of protein kinase C-${\alpha}$ was more intense in epidermis, secondary hair germ cells, outer root sheath in all experimental groups than control group. Expression of vascular endothelial growth factor was more intense in epidermis, bulge, secondary hair germ cells, outer root sheath in plum-blossom needle treated group and strong moxibustion treated group than control group. We concluded that acupuncture & moxibustion therapy related to the expression of various growth factors, enzymes and receptor on the hair growth cycle for hair growth.

Keywords

References

  1. Headington, J.T. Histological findings in androgenic alopecia treated with topical minoxidil. Br J Dermatol 107(Suppl. 22):20-21, 1982.
  2. 문정배, 김영진, 이태후. 한의학적 탈모 치료효과의 객관적 평가 방법. 대한한의학회지 27(2):57-69, 2006.
  3. 이태후, 문정배, 안경애, 이혜정. 탈모 환자의 적외선 체열 진단상 안면부 체열 특성. 대한한의학회지 27(2):249-252, 2006.
  4. 윤신혁. 다양한 발모성장 물질에 대한 동물실험 및 분자생물학적 접근:아젤라인산 및 비타민 B6의 효과. 대구가톨릭대학교 대학원 석사학위논문, 2007.
  5. 李元文, 张韦川. 名医临床证经验丛书皮肤病. 北京. 人民衛生出版社. p 572, 2001.
  6. 李元文, 张韦川. 名医临床經叢書 皮肤病. 北京. 人民衛生出版社. p 618, 2001.
  7. Muller, H.K., Rook, A.J., Kubba, R. Immunohistology and auto antibody studies in alopecia areata. Br J Dermatol 102: 609-610, 1980. https://doi.org/10.1111/j.1365-2133.1980.tb07664.x
  8. Bergfeld, W. Diffuse hair loss in women. Cutis 22: 190-195, 1978.
  9. Selmanowilz, V., Victor, S., Warburton, D., Orentreich, N. Fingerprints arches in alopecia areata. Arch Dermatol 110: 570-571, 1974. https://doi.org/10.1001/archderm.1974.01630100030007
  10. Friedman, P.S. Alopecia areata and auto-immunity. Br J Dermatol 105: 153-157, 1981.
  11. Rashtak, S., Pittelkow, M.R. Skin involvement in systemic autoimmune diseases. Curr Dir Autoimmun 10: 344-358, 2008.
  12. Toback, C., Ajkunar, S. The emotional disturbances underlying alopecia areata, alopecia totalis and trichotillomania. Child Psychiatry Hum Dev 10: 114-117, 1979. https://doi.org/10.1007/BF01433503
  13. 홍석훈, 노석선. 모발탈락의 병인 및 치료법에 대한 문헌적 고찰. 외관과학회지 9(1):43-65, 1996.
  14. 임사비나, 최규동, 김수경. 탈모에 대한 동서의학적 고찰. 경산대학교 제한동의학술원 논문집 4(1):699-710, 1999.
  15. 송순기, 이명종. 안드로겐성 탈모증의 두피재활에 관한 문헌적 고찰. 한의정보학회지 5(1):162-176, 1999.
  16. 김소영, 김중호, 채병운. 탈모에 응용되는 신응양진단의 효능에 관한 실험적 연구. 외관과학회지 4(1):43-58, 1991.
  17. 권강주, 이창현, 이문원, 문연자, 최두호, 이학재, 김호민. 사물탕가미방이 자발적인 원형탈모생쥐의 발모효과에 관한 실험적 연구. 동의생리병리학회지 19(3):696-704, 2005.
  18. 김필수. 당귀보혈탕가미방이 모발탈락 및 성정에 미치는 영향. 세명대학교대학원 박사학위논문, 2004.
  19. 노현찬, 노석선. 고삼추출물이 모발성장 촉진 및 면포억제에 미치는 영향. 대한안이비인후피부과학회지 15(1):96-126, 2002.
  20. 정석영, 정일국, 김대근, 조한영, 정한솔, 이창현. C57BL/6N 생쥐에서 골쇄보 추출물을 이용한 발모효과에 대한 실험적 연구. 동의생리병리학회지 21(2):453-461, 2007.
  21. 윤정훈, 기경헌, 장수진, 신민섭. 약침을 이용한 원형탈모증 치험 1예. 대한외관과학회지 14(1):1005-1010, 2001.
  22. 김경운, 서정철, 이경민, 임성철, 정태영, 한상원. 봉약침과 홍화약침을 이용한 원형탈모증 치험 1예. 대한약침학회지 7(2):91-96, 2004.
  23. Civatte, J. Topical 2% minoxidil solution in mail pattern alopecia. The initial European Experience. Int J Dermatol 27: 424, 1988. https://doi.org/10.1111/j.1365-4362.1988.tb00002.x
  24. Drake, L., Hordinsky, M., Fiedler, V., Swinehart, J., Unger, W.P., Cotterill, P.C., Thiboutot, D.M., Lowe, N., Jacobson, C., Whiting, D., Stieglitz, S., Kraus, S.J., Griffin, E.I., Weiss, D., Carrington, P., Gencheff, C., Cole, G.W., Pariser, D.M., Epstein, E.S., Tanaka, W., Dallob, A., Vandormael, K., Geissler, L., Waldstreicher, J. The effects of finasteride on scalp skin and serum androgen levels in men with androgenetic alopecia. J Am Acad Dermatol 41(4):550-554, 1999.
  25. Hillen, H.F., Breed, W.P, Botman, C.J. Scalp cooling by cold air for the prevention of chemotherapy-induced alopecia. Neth J Med 37(5-6):231-235, 1990.
  26. Schuttelaar, M.L., Hamstra, J.J., Plinck, E.P., Peereboom-Wynia, J.D., Vuzevski, V.D., Mulder, P.G., Oranje, A.P. Alopecia areata in children : treatment with diphencyprone. Br J Dermatol 135(4):581-585, 1996. https://doi.org/10.1111/j.1365-2133.1996.tb03835.x
  27. 서의후, 왕보방, 장영. 피부병중의진료학, 인민위생출판사, pp 61-65, 1997.
  28. Hsu, S.M., Raine, L., Fanger, H. Use of avidin-biotin-peroxidase complex(ABC) in immunoperoxidase techniques: A comparison between ABC and unlabeled antibody(PAP) procedures. J Histochem Cytochem 29: 577-580, 1981. https://doi.org/10.1177/29.4.6166661
  29. Dry, F.W., The coat of mouse(mus musculus). J Genet 16: 197-340, 1926. https://doi.org/10.1007/BF02982998
  30. Paus, R., Foitzik, K. In search of the ""hair cycle clock"": A guided tour. Differentiation 72: 489-511, 2004. https://doi.org/10.1111/j.1432-0436.2004.07209004.x
  31. Taylor, G., Lehrer, M.S., Jensen, P.J., Sun, T.T. & Lavker, R.M. Involvement of follicular stem cells in forming not only the follicle but also the epidermis. Cell 102: 451-461, 2000. https://doi.org/10.1016/S0092-8674(00)00050-7
  32. Oshima, H., Rochat, A., Kedzia, C., Kobayashi, K. & Barrandon, Y. Morphogenesis and renewal of hair follicles from adult multipotent stem cells. Cell 104: 233-245, 2001. https://doi.org/10.1016/S0092-8674(01)00208-2
  33. 馬绍尧, 赵尚華. 현대중의피부성병진료대전, 산서과학기술출판사, pp 956-967, 1998.
  34. 李正泰, 李秉烈. 黃帝內經에 나타난 灸의 이론에 관한 연구. 대한침구학회지 16: 79-90, 1999.
  35. Tohya, K., Urabe, S., Igarashi, J., Tomura, T., Take, A., Kimura, M. Appearance of peculiar vessels with immunohistoligical features of high endothelial venules in the dermis of moxibustion-stimulated rat skin. Am J Chin Med 28: 425-433, 2000. https://doi.org/10.1142/S0192415X00000507
  36. Okazaki, M., Aizawa, S., Yamauchi, M., Oguchi, K. Effect of single moxibustion on cutaneous blood vessel and microvascular permeability in mice. Am J Chin Med 18: 121-130, 1990. https://doi.org/10.1142/S0192415X90000162
  37. 秦万章. 現代中醫藥應用與硏究大系, 12권, 皮膚科, 上海中醫葯大學出版社, pp 270-282, 1999.
  38. 黃霏莉, 余靖. 中醫美容學, 2版, 北京, 人民衛生出版社, p 194, 2004.
  39. Argyris, T.S. The effects of wounds on adjacent growing or resting hair follicles in mice. Arch Dermatol Symp 61: 31-36, 1956.
  40. Argyris, T.S. Kinetics of epidermal production during epidermal regeneration following abrasion in mice. Am J Pathol 83: 329-340, 1956.
  41. Silver, A.F., Chase, H.B., Arsenault, C.T. Early anagen initiated by plucking compared with early spontaneous anagen. Adv Biol Skin 9: 265-286, 1969.
  42. Stenn, K.S., Paus, R. Controls of hair follicle. Physiological Reviews 81: 449-494, 2001. https://doi.org/10.1152/physrev.2001.81.1.449
  43. Oshima, H., Rochat, A., Kedzia, C., Kobayashi, K., Barrandon, Y. Morphogenesis and renewal of hair follicles from adult multipotent stem cells. Cell 104: 233-245, 2001. https://doi.org/10.1016/S0092-8674(01)00208-2
  44. Pearson, A.J., Ashby, M.G., Wildermoth, J.E., Craven, A.J., Nixon, A.J. Effect of exogenous prolactin on the hair growth cycle. Exp Dermatol 8: 358-360, 1999.
  45. Yano, K., Brown, L.F., Detmar, M. Control of hair growth and follicle size by VEGF-mediated angiogenesis. J Clin Invest 107: 409-417, 2001. https://doi.org/10.1172/JCI11317
  46. Dvorak, H.F., Brown, L.F., Detmar, M., Dvorak, AM. Vascular permeability factor/vascular endothelial growth factor, microvascular hyperpermeability, and angiogenesis. Am J Pathol 146: 1029-1039, 1995.
  47. Ozeki, M., Tabata, Y. Promoted growth of murine hair follicle through controlled release of vascular endothelial growth factor. Biomaterials 23: 2367-2373, 2002. https://doi.org/10.1016/S0142-9612(01)00372-6
  48. Peters, E.M., Maurer, M., Botchkarev, V.A., Jensen, K., Welker, P., Scott, G.A., Paus, R. Kit is expressed by epithelial cells in vivo. J Invest Dermatol 121: 976-984, 2003.
  49. Li, L.F., Guo, J., Gao, Z.F. Overexpression of skin protein kinase C-alpha in anagen hair follicles during induced growth of mouse hair. Clin Exp Dermatol 28: 429-433, 2003. https://doi.org/10.1046/j.1365-2230.2003.01328.x
  50. Tatefuji, T., Arai, C., Mori, T., Okuda, Y., Kayano, T., Mizote, A., Okura, T., Takeuchi, M., Ohta, T., Kurimoto, M. The Effect of AgK114 on wound Healing. Biol Pharm Bull 29(5):896-902, 2006. https://doi.org/10.1248/bpb.29.896