Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effect of the Water Extract of Cultivated Wild Ginseng on the Toxicities Induced by Cyclophosphamide in Mice

산양삼이 Cyclophosphamide의 독성에 미치는 영향

  • Lee, Sung-Woo (Department of Internal Medicine, College of Oriental Medicine, Semyung University) ;
  • Kim, Ki-Tae (Department of Internal Medicine, College of Oriental Medicine, Semyung University) ;
  • Ko, Heung (Department of Internal Medicine, College of Oriental Medicine, Semyung University)
  • 이성우 (세명대학교 한의과대학 내과학교실) ;
  • 김기태 (세명대학교 한의과대학 내과학교실) ;
  • 고흥 (세명대학교 한의과대학 내과학교실)
  • Received : 2010.11.19
  • Accepted : 2011.10.15
  • Published : 2011.10.25
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Abstract

This study was investigated the protective effect of Cultivated Wild Ginseng(WG) on the toxicities induced by cyclophosphamide(CP) in mice. Twenty-four male BALB/c mice were divided into non-treated normal group(n=6), CP treated control group(n=6), CP+WG treated CP+WG group(n=6), WG treated WG group(n=6). CP(100 mg/kg of b.w., i.p) was injected at 0, 7 th, 14 th, 21 th, and 28 th day of the experiment respectively. WG(4.4 g/kg, i.p.) was administrated for 35days. Body and organ(heart, liver, kidney, testis) weight were measured. Histopathological examination on the organ(heart, liver, kidney, testis), morphometric analysis, and BrdU immunohistochemistry on the testis were performed. Body weight was decreased following CP administration. In contrast, such a decrease was significantly attenuated by WG administration. CPK and AST of CP+WG group were significantly decreased compared with CP group. Histopathologically, cross sectional area of testis and diameter of seminiferous tubule were significantly increased in CP+WG group compared with CP group. BrdU labelled cells in the seminiferous tubules were remarkably decreased in CP group. Whereas the number of seminiferous tubules labelled with BrdU in spermatogonia was increased by CP+WG administration. The obtained results suggest that WG has protective effect on CP-induced toxicity. This effect might be mediated through the supplementation of vital energy.

Keywords

References

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