DOI QR코드

DOI QR Code

Lack of Influence of MGMT Codon Leu84Phe and Codon Ileu143Val Polymorphisms on Esophageal Cancer Risk in the Kashmir Valley

  • Shah, Mohd A. (Department of Clinical Biochemistry, Sher-i-Kashmir Institute of Medical Sciences) ;
  • Shaffi, Sheikh M. (Department of Clinical Biochemistry, Sher-i-Kashmir Institute of Medical Sciences) ;
  • Lone, Ghulam Nabi (Department of Cardio-vascular and Thoracic Surgery, Sher-i-Kashmir Institute of Medical Sciences) ;
  • Jan, Syed Mudassar (Department of Clinical Biochemistry, Sher-i-Kashmir Institute of Medical Sciences)
  • 발행 : 2012.07.31

초록

The enzyme encoded by the MGMT gene is involved in the repair of alkylated lesions formed in DNA by carcinogenic nitrosamines. Since dietary items consumed by the Kashmiri population contain high concentrations of these agents, it is biologically plausible that MGMT polymorphic variants may be associated with their risk of esophageal cancer. The present study was performed to assess whether non-synonymous SNPS at codon Leu84Phe and codon Ileu143Val of the MGMT gene, close to the active site of the protein, might be linked to predisposition of Kashmiris to esophageal cancer. Genotyping was carried out by polymerase chain reaction-restriction fragment length polymorphism on 92 cases and 77 healthy controls. Codon 84 and codon 143 SNPs of the MGMT gene were not associated with any increase in risk. While the frequency of the Phe allele at codon 84 in cases was (0.16), slightly higher than controls (0.12), the difference was not statistically significant. Similarly, the frequency of Valine allele in cases at codon 143 (0.08) and controls (0.09) was nearly equal. Moreover, no significant association of MGMT genotypes with the clinicopatholgic variables of esophageal cancer patients was observed. In conclusion, MGMT variants at codon 84 and codon143 may not be involved in the susceptibility of the Kashmiri population to esophageal cancer.

키워드

참고문헌

  1. Akbari MR, Malekzadeh R, Shakeri R, et al (2009). Candidate gene association study of esophageal squamous cell carcinoma in a high-risk region in Iran. Cancer Res, 69, 7994-8000. https://doi.org/10.1158/0008-5472.CAN-09-1149
  2. Bartsch H, Montesano R (1984). Relevance of nitrosamines to human cancer. Carcinogenesis, 5, 1381-93. https://doi.org/10.1093/carcin/5.11.1381
  3. Bugni JM, Han J, Tsai M, Hunter DJ, Samson LD (2007). Genetic association and functional studies of major polymorphic variants of MGMT. DNA Repair, 6, 1116-26. https://doi.org/10.1016/j.dnarep.2007.03.023
  4. Cohet C, Borel S, Nyberg F, et al (2004). Exon 5 polymorphisms in the O6-alkylguanine DNA alkyltransferase gene and lung cancer risk in non-smokers exposed to second-hand smoke. Cancer Epidemiol Biomarkers Prev, 13, 320-3. https://doi.org/10.1158/1055-9965.EPI-03-0120
  5. Doecke J, Zhao ZZ, Pandeya N, et al (2008). Polymorphisms in MGMT and DNA repair genes and the risk of esophageal adenocarcinoma. Int J Cancer, 123, 174-80. https://doi.org/10.1002/ijc.23410
  6. Esteller M, Herman JG (2004). Generating mutations but providing chemosensitivity: the role of O6-methylguanine DNA methyltransferase in human cancer. Oncogene, 23, 1-8. https://doi.org/10.1038/sj.onc.1207316
  7. Goth R, Rajewsky MF (1974). Persistence of O6-ethylguanine in rat-brain DNA: correlation with nervous system-specific carcinogenesis by ethylnitrosourea. Proc Natl Acad Sci, 71, 639-43. https://doi.org/10.1073/pnas.71.3.639
  8. Hall J, Hashibe M, Boffetta P, et al (2007). The association of sequence variants in DNA repair and cell cycle genes with cancers of the upper aerodigestive tract. Carcinogenesis, 28, 665-71.
  9. Khuroo MS, Zargar SA, Mahajan R, Banday MA (1992). High incidence of esophageal and gastric cancer in Kashmir a population with special dietary and personal habits. Gut, 33, 11-5. https://doi.org/10.1136/gut.33.1.11
  10. Loveless A (1969). Possible relevance of $O^6$ alkylation of deoxyguanosine to the mutagenicity and carcinogenicity of nitrosamines and nitrosamides. Nature, 223, 206-7. https://doi.org/10.1038/223206a0
  11. N. Blin, DW Stafford (1976). A general method for isolation of high molecular weight DNA from eukaryotes, Nucleic Acids Res, 3, 2303-8. https://doi.org/10.1093/nar/3.9.2303
  12. Pegg AE, Fang Q, Loktionova NA (2007). Human variants of O6-alkylguanine-DNA alkyltransferase. DNA repair, 6, 1071-8. https://doi.org/10.1016/j.dnarep.2007.03.012
  13. Shen J, Terry MB, Gammon MD, et al (2005). MGMT genotype modulates the associations between cigarette smoking, dietary antioxidants and breast cancer risk. Carcinogenesis, 26, 2131-7. https://doi.org/10.1093/carcin/bgi179
  14. Siddiqi M, Preussmann R (1989). Esophageal cancer in Kashmir-an assessment. J Cancer Res Clin Oncol, 115, 111-7. https://doi.org/10.1007/BF00397910
  15. Swann F, Magee PN (1968). Nitrosamine-induced carcinogenesis. The alkylation of nucleic acids of the rat by N-methyl-Nnitrosourea, dimethylnitrosamine, dimethylsulphate and methylmethane sulphonate. Biochemistry, 110, 39-47.
  16. Tranah GJ, Bugni J, Giovannucci E, et al (2006). $O^6$-Methylguanine-DNA methyltransferase Leu84Phe and Ile143Val polymorphisms and risk of colorectal cancer in the Nurses' Health Study and Physicians' Health Study (United States), Cancer Causes Control, 17, 721-31. https://doi.org/10.1007/s10552-006-0005-y
  17. Wen-Jing Ma, Guo-Dong Lv, Shu-Tao Zheng, et al (2010). DNA polymorphism and risk of esophageal squamous cell carcinoma in a population of North Xinjiang, China. World J Gastroenterol, 16, 641-7. https://doi.org/10.3748/wjg.v16.i5.641
  18. Xing D, Tan W, Lin D (2003). Genetic polymorphisms and susceptibility to esophageal cancer among Chinese population (review). Oncol Rep, 10, 1615-23.
  19. Zhong Y, Huang Y, Huang Y, et al (2010). Effects of O6-methylguanine-DNA methyltransferase (MGMT) polymorphisms on cancer: a meta-analysis. Mutagenesis, 1, 83-95.

피인용 문헌

  1. The polymorphisms in the MGMT gene and the risk of cancer: a meta-analysis vol.34, pp.5, 2013, https://doi.org/10.1007/s13277-013-0893-x
  2. Two DNA repair gene polymorphisms on the risk of gastrointestinal cancers: a meta-analysis vol.35, pp.3, 2014, https://doi.org/10.1007/s13277-013-1320-z
  3. Genetic unraveling of colorectal cancer vol.35, pp.6, 2014, https://doi.org/10.1007/s13277-014-1713-7
  4. Correlations of MGMT genetic polymorphisms with temozolomide resistance and prognosis of patients with malignant gliomas: a population-based study in China vol.24, pp.5, 2017, https://doi.org/10.1038/cgt.2017.7
  5. rs12917 polymorphism in cancer risk: an updated pooling analysis with 21010 cases and 34018 controls vol.38, pp.5, 2018, https://doi.org/10.1042/BSR20180942