Asian Pacific Journal of Cancer Prevention

  • 제13권10호
  • /
  • Pages.4993-4998
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  • 2012
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  • 1513-7368(pISSN)
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  • 2476-762X(eISSN)
아시아태평양암예방학회 (Asian Pacific Journal of Cancer Prevention)
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Genotoxic and Anti-Genotoxic Effects of Vanillic Acid Against Mitomycin C-Induced Genomic Damage in Human Lymphocytes In Vitro

  • Erdem, Merve Guler (Department of Biology, Faculty of Science and Arts, Cell Culture and Genetic Toxicology Laboratory) ;
  • Cinkilic, Nilufer (Department of Biology, Faculty of Science and Arts, Cell Culture and Genetic Toxicology Laboratory) ;
  • Vatan, Ozgur (Department of Biology, Faculty of Science and Arts, Cell Culture and Genetic Toxicology Laboratory) ;
  • Yilmaz, Dilek (Department of Biology, Faculty of Science and Arts, Cell Culture and Genetic Toxicology Laboratory) ;
  • Bagdas, Deniz (Faculty of Medicine, Uludag University, Experimental Animals Breeding and Research Center) ;
  • Bilaloglu, Rahmi (Department of Biology, Faculty of Science and Arts, Cell Culture and Genetic Toxicology Laboratory)
  • 발행 : 2012.10.31
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초록

Vanillic acid, a vegetable phenolic compound, is a strong antioxidant. The aim of the present study was to determine its effects on mitomycin C-induced DNA damage in human blood lymphocyte cultures in vitro, both alone and in combination with mitomycin C (MMC). The cytokinesis block micronucleus test and alkaline comet assay were used to determine genotoxic damage and anti-genotoxic effects of vanillic acid at the DNA and chromosome levels. MMC induced genotoxicity at a dose of $0.25{\mu}g/ml$. Vanillic acid ($1{\mu}g/ml$) significantly reduced both the rates of DNA damaged cells and the frequency of micronucleated cells. A high dose of vanillic acid ($2{\mu}g/ml$) itself had genotoxic effects on DNA. In addition, both test systems showed similar results when tested with the negative control, consisting of dimethyl sulfoxide (DMSO) in combination with vanillic acid ($1{\mu}g/ml$)+MMC. In conclusion, vanillic acid could prevent oxidative damage to DNA and chromosomes when used at an appropriately low dose.

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참고문헌

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