Asian Pacific Journal of Cancer Prevention

  • 제13권11호
  • /
  • Pages.5709-5714
  • /
  • 2012
  • /
  • 1513-7368(pISSN)
  • /
  • 2476-762X(eISSN)
아시아태평양암예방학회 (Asian Pacific Journal of Cancer Prevention)
권호 표지
DOI QR코드

DOI QR Code

Curcumin Inhibits TGF-β1-Induced MMP-9 and Invasion through ERK and Smad Signaling in Breast Cancer MDA-MB-231 Cells

  • Mo, Na (Department of Pathology, Chongqing Medical University) ;
  • Li, Zheng-Qian (Department of Anesthesiology, Peking University Third Hospital (PUTH)) ;
  • Li, Jing (Department of Pathology, Chongqing Medical University) ;
  • Cao, You-De (Department of Pathology, Chongqing Medical University)
  • 발행 : 2012.11.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objective: To evaluate the effects of curcumin on matrixmetalloproteinase-9 (MMP-9) and invasion ability induced by transforming growth factor-${\beta}1$ (TGF-${\beta}1$) in MDA-MB-231 cells and potential mechanisms. Methods: Human breast cancer MDA-MB-231 cells were used with the CCK-8 assay to measure the cytotoxicity of curcumin. After treatment with 10 ng/ml TGF-${\beta}1$, with or without curcumin (${\leq}10{\mu}M$), cell invasion was checked by transwell chamber. The effects of curcumin on TGF-${\beta}1$-stimulated MMP-9 and phosphorylation of Smad2, extracellular-regulated kinase (ERK), and p38 mitogen activated protein kinases (p38MAPK) were examined by Western blotting. Supernatant liquid were collected to analyze the activity of MMP-9 via zymography. Following treatment with PD98059, a specific inhibitor of ERK, and SB203580, a specific inhibitor of p38MAPK, Western blotting and zymography were employed to examine MMP-9 expression and activity, respectively. Results: Low dose curcumin (${\leq}10{\mu}M$) did not show any obvious toxicity to the cells, while $0{\sim}10{\mu}mol/L$ caused a concentration-dependent reduction in cell invasion provoked by TGF-${\beta}1$. Curcumin also markedly inhibited TGF-${\beta}1$-regulated MMP-9 and activation of Smad2, ERK1/2 and p38 in a dose- and time-dependent manner. Additionally, PD98059, but not SB203580, showed a similar pattern of inhibition of MMP-9 expression. Conclusion: Curcumin inhibited TGF-${\beta}1$-stimulated MMP-9 and the invasive phenotype in MDA-MB-231 cells, possibly associated with TGF-${\beta}$/Smad and TGF-${\beta}$/ERK signaling.

키워드

참고문헌

  1. Allen M, Louise Jones J (2011). Jekyll and Hyde: the role of the microenvironment on the progression of cancer. J Pathol, 223, 162-76.
  2. Bakin AV, Safina A, Rinehart C, et al (2004). A critical role of tropomyosins in TGF-beta regulation of the actin cytoskeleton andcell motility in epithelial cells. Daroqui C, Darbary H, Helfman DM. Mol Biol Cell, 15, 4682-94. https://doi.org/10.1091/mbc.E04-04-0353
  3. Belotti D, Calcagno C, Garofalo A (2008). Vascular endothelial growth factor stimulates organ-specific host matrix metalloproteinase-9 expression and ovarian cancer invasion. Mol Cancer Res, 6, 525-34. https://doi.org/10.1158/1541-7786.MCR-07-0366
  4. Biswas S, Guix M, Rinehart C, et al (2007). Inhibition of TGFbeta with neutralizing antibodies prevents radiation-induced acceleration of metastatic cancer progression. J Clin Invest, 117, 1305-13. https://doi.org/10.1172/JCI30740
  5. Blair KJ, Kiang A, Wang-Rodriguez J, et al (2011). EGF and bFGF promote invasion that is modulated by PI3/Akt kinase and Erk in vestibular schwannoma. Otol Neurotol, 32, 308-14. https://doi.org/10.1097/MAO.0b013e318206fc3d
  6. Chen P, Lu N, Ling Y, Chen Y, et al (2011). Inhibitory effects of wogonin on the invasion of human breast carcinoma cells by downregulating the expression and activity of matrix me talloproteinase-9. Toxicology, 282, 122-8. https://doi.org/10.1016/j.tox.2011.01.018
  7. Cheung KL (2007). Endocrine therapy for breast cancer: an overview. Breast, 16, 327-43. https://doi.org/10.1016/j.breast.2007.03.004
  8. Chou YT, Wang H, Chen Y (2006). Cited2 modulates TGF-betamediated upregulation of MMP9. Oncogene, 25, 5547-60. https://doi.org/10.1038/sj.onc.1209552
  9. Dziembowska M, Danilkiewicz M, Wesolowska A (2007). Cross-talk between Smad and p38 MAPK signalling in transforming growth factor beta signal transduction in human glioblastoma cells. Biochem Biophys Res Commun, 354, 1101-6. https://doi.org/10.1016/j.bbrc.2007.01.113
  10. Hassan ZK, Daghestani MH (2012). Curcumin effect on MMPs and TIMPs genes in a breast cancer cell line. Asian Pac J Cancer Prev, 13, 3259-64. https://doi.org/10.7314/APJCP.2012.13.7.3259
  11. Hortobagyi GN (2002). The status of breast cancer management: challenges and opportunities. Breast Cancer Res Treat, 75, S61-5, discussion S57-9. https://doi.org/10.1023/A:1020326219576
  12. Hsieh HL, Wang HH, Wu WB, et al (2010). Transforming growth factor-${\beta}$1 induces matrix metalloproteinase-9 and cell migration in astrocytes: roles of ROS-dependent ERK-and JNK-NF-${\kappa}B$ pathways. J Neuroinflammation, s, 88.
  13. Hedges JC, Dechert MA, Yamboliev IA, et al (1999).A role for p38(MAPK)/HSP27 pathway in smooth muscle cell migration. J Biol Chem, 274, 24211-9. https://doi.org/10.1074/jbc.274.34.24211
  14. Helfman DM, Pawlak G (2004). Myosin light chain kinase and acto-myosin contractility modulate activation of theERK cascade downstream of oncogenic Ras. J Cell Biochem, 95, 1069-80.
  15. IIunga K, Nishiura R, Inada H (2004). Co-stimulation of human breast cancer cells with transforming growth factor-beta and tenascin-C enhances matrix metalloproteinase-9 expression and cancer cell invasion. Int J Exp Pathol, 85, 373-9 https://doi.org/10.1111/j.0959-9673.2004.00406.x
  16. Imamura T, Hikita A, Inoue Y (2012). The roles of TGF-${\beta}$ signaling in carcinogenesis and breast cancer metastasis. Breast Cancer, 19, 118-24. https://doi.org/10.1007/s12282-011-0321-2
  17. Geiger TR, Peeper DS (2009). Metastasis mechanisms. Biochim Biophys Acta, 1796, 293-308.
  18. Kim HS, Luo L, Pflugfelder SC, Li DQ (2005). Doxycycline inhibits TGF-beta1-induced MMP-9 via Smad and MAPK pathways in human corneal epithelial cells. Invest Ophthalmol Vis Sci, 46, 840-8. https://doi.org/10.1167/iovs.04-0929
  19. Na D, Lv ZD, Liu FN, et al (2010). Transforming growth factor beta1 produced in autocrine/ paracrine manner affects the morphology and function of mesothelial cells and promotes peritoneal carcinomatosis. Int J Mol Med, 26, 325-32.
  20. Harhra NA, Basaleem HO (2012). Trends of breast cancer and its management in the last twenty years in aden and adjacent governorates, yemen. Asian Pac J Cancer Prev, 13, 4347-51 https://doi.org/10.7314/APJCP.2012.13.8.4247
  21. Park J, Ayyappan V, Bae EK, et al (2008). Curcumin in combination with bortezomib synergistically induced apoptosis in human multiple myeloma U266 cells. Mol Oncol, 2, 317-26. https://doi.org/10.1016/j.molonc.2008.09.006
  22. Perera M, Tsang CS, Distel RJ, et al (2010). TGF-beta1 interactome: metastasis and beyond. Cancer Genomics Proteomics, 7, 217-29.
  23. Porter PL(2009). Global trends in breast cancer incidence and mortality. Salud Publica Mex, 51, S141-6. https://doi.org/10.1590/S0036-36342009000800003
  24. Safina A, Vandette E, Bakin AV (2007). ALK5 promotes tumor angiogenesis by upregulating matrix metalloproteinase-9 in tumor cells. Oncogene, 26, 2407-22. https://doi.org/10.1038/sj.onc.1210046
  25. Sanchez-Zamorano LM, Flores-Luna L, Angeles-Llerenas A et al (2011). Healthy lifestyle on the risk of breast cancer. Cancer Epidemiol Biomarkers Prev, 20, 912-22. https://doi.org/10.1158/1055-9965.EPI-10-1036
  26. Santibanez JF, Guerrero J, Quintanilla M, et al (2002). Transforming growth factor-beta1 modulates matrix metalloproteinase-9 production through the Ras/MAPK signaling pathway in transformed keratinocytes. Biochem Biophys Res Commun, 16, 267-73.
  27. Santibanez JF, Quintanilla M, Martinez J (2000). Genistein and curcumin block TGF-beta 1-induced u-PA expression and migratory and invasive phenotype in mouse epidermal keratinocytes. Nutr Cancer, 37, 49-54. https://doi.org/10.1207/S15327914NC3701_6
  28. Serra R, Crowley MR (2005). Mouse models of transforming growth factor beta impact in breast development and cancer. Endocr Relat Cancer, 12, 749-60. https://doi.org/10.1677/erc.1.00936
  29. Smith AL, Robin TP, Ford HL (2012). Molecular Pathways: Targeting the TGF-beta Pathway for Cancer Therapy. Clin Cancer Res, 18, 4514-21. https://doi.org/10.1158/1078-0432.CCR-11-3224
  30. Smith MR, Gangireddy SR, Narala VR, et al (2010). Curcumin inhibits fibrosis-related effects in IPF fibroblasts and in mice following bleomycin-induced lung injury. Am J Physiol Lung Cell Mol Physiol, 298, L616-25. https://doi.org/10.1152/ajplung.00002.2009
  31. Srinivasan R, Forman S, Quinlan RA, et al (2008).Regulation of contractility by Hsp27 and Hic-5 in rat mesenteric small arteries. Am J Physiol Heart Circ Physiol, 294, H961-9. https://doi.org/10.1152/ajpheart.00939.2007
  32. Szuster-Ciesielska A, Plewka K, Daniluk J (2011). Betulin and betulinic acid attenuate ethanol-induced liver stellate cell activation by inhibiting reactive oxygen species (ROS), cytokine (TNF-alpha, TGF-beta) production and by influencing intracellular signaling. Toxicology, 280, 152-63. https://doi.org/10.1016/j.tox.2010.12.006
  33. Yan G, Graham K, Lanza-Jacoby S (2012). Curcumin enhances the anticancer effects of trichostatin a in breast cancer cells. Mol Carcinog.
  34. Yao QY, Xu BL, Wang JY, et al (2012). Inhibition by curcumin of multiple sites of the transforming growth factor-beta1 signalling pathway ameliorates the progression of liver fibrosis induced by carbon tetrachloride in rats. BMC Complement Altern Med, 12, 156. https://doi.org/10.1186/1472-6882-12-156
  35. Ye B, Jiang LL, Xu HT, et al (2012). Expression of PI3K/AKT pathway in gastric cancer and its blockade suppresses tumor growth and metastasis. Int J Immunopathol Pharmacol, 25, 627-36. https://doi.org/10.1177/039463201202500309
  36. Yodkeeree S, Ampasavate C, Sung B, et al (2010). curcumin suppresses migration and invasion of MDA-MB-231 humanbreast cancer cell line. Eur J Pharmacol, 627, 8-15. https://doi.org/10.1016/j.ejphar.2009.09.052
  37. Zayani Y, Allal-Elasmi M, Jacob MP, et al (2012).Abnormal circulating levels of matrix metalloprotei-nases and their inhibitors in diabetes mellitus. Clin La, 58, 779-85.
  38. Zhang SS, Gong ZJ, Li WH, et al (2012). Antifibrotic effect of curcumin in TGF-beta 1-induced myofibroblasts from human oral mucosa. Asian Pac J Cancer Pre, 13, 289-94. https://doi.org/10.7314/APJCP.2012.13.1.289
  39. Zhang YE (2009). Non-Smad pathways in TGF-beta signaling. Cell Res, 19, 128-39. https://doi.org/10.1038/cr.2008.328

피인용 문헌

  1. Co-treatment of THP-1 cells with naringenin and curcumin induces cell cycle arrest and apoptosis via numerous pathways vol.12, pp.6, 2015, https://doi.org/10.3892/mmr.2015.4480
  2. AKT1 Inhibitory DNAzymes Inhibit Cell Proliferation and Migration of Thyroid Cancer Cells vol.14, pp.4, 2013, https://doi.org/10.7314/APJCP.2013.14.4.2571
  3. Curcumin Analogue A501 induces G2/M Arrest and Apoptosis in Non-small Cell Lung Cancer Cells vol.15, pp.16, 2014, https://doi.org/10.7314/APJCP.2014.15.16.6893
  4. Molecular mechanism of TGF-β signaling pathway in colon carcinogenesis and status of curcumin as chemopreventive strategy vol.35, pp.8, 2014, https://doi.org/10.1007/s13277-014-1840-1
  5. Breast cancer chemoprevention by dietary natural phenolic compounds: Specific epigenetic related molecular targets vol.59, pp.1, 2014, https://doi.org/10.1002/mnfr.201400515
  6. pTyr421 Cortactin Is Overexpressed in Colon Cancer and Is Dephosphorylated by Curcumin: Involvement of Non-Receptor Type 1 Protein Tyrosine Phosphatase (PTPN1) vol.9, pp.1, 2014, https://doi.org/10.1371/journal.pone.0085796
  7. Curcumin Inhibits the Proliferation and Invasiveness of MHCC97-H Cells via p38 Signaling Pathway pp.02724391, 2014, https://doi.org/10.1002/ddr.21210
  8. Curcumin and Emodin Down-Regulate TGF-β Signaling Pathway in Human Cervical Cancer Cells vol.10, pp.3, 2015, https://doi.org/10.1371/journal.pone.0120045
  9. Curcumin inhibits the invasion of lung cancer cells by modulating the PKCα/Nox-2/ROS/ATF-2/MMP-9 signaling pathway vol.34, pp.2, 2015, https://doi.org/10.3892/or.2015.4044
  10. Suppression of TPA-induced cancer cell invasion by Peucedanum japonicum Thunb. extract through the inhibition of PKCα/NF-κB-dependent MMP-9 expression in MCF-7 cells vol.37, pp.1, 2015, https://doi.org/10.3892/ijmm.2015.2417
  11. To study the effect of curcumin on the growth properties of circulating endothelial progenitor cells vol.51, pp.5, 2015, https://doi.org/10.1007/s11626-014-9852-0
  12. Curcumin induces apoptosis and suppresses invasion through MAPK and MMP signaling in human monocytic leukemia SHI-1 cells pp.1744-5116, 2015, https://doi.org/10.3109/13880209.2015.1060508
  13. Curcumin suppresses migration and invasion of human endometrial carcinoma cells pp.1792-1082, 2015, https://doi.org/10.3892/ol.2015.3478
  14. Curcumin inhibits LPA-induced invasion by attenuating RhoA/ROCK/MMPs pathway in MCF7 breast cancer cells vol.16, pp.1, 2016, https://doi.org/10.1007/s10238-015-0336-7
  15. Epo inhibits the fibrosis and migration of Müller glial cells induced by TGF-β and high glucose vol.254, pp.5, 2016, https://doi.org/10.1007/s00417-016-3290-5
  16. Phosphorylated-p38 mitogen-activated protein kinase expression is associated with clinical factors in invasive breast cancer vol.5, pp.1, 2016, https://doi.org/10.1186/s40064-016-2636-0
  17. Therapeutic potential of novel formulated forms of curcumin in the treatment of breast cancer by the targeting of cellular and physiological dysregulated pathways vol.233, pp.3, 2017, https://doi.org/10.1002/jcp.25961
  18. Intracellular signaling pathways of inflammation modulated by dietary flavonoids: The most recent evidence pp.1549-7852, 2017, https://doi.org/10.1080/10408398.2017.1345853
  19. Salvia miltiorrhiza extract inhibits TPA-induced MMP-9 expression and invasion through the MAPK/AP-1 signaling pathway in human breast cancer MCF-7 cells vol.14, pp.3, 2017, https://doi.org/10.3892/ol.2017.6638
  20. Molecular Mechanisms Underlying Curcumin-Mediated Therapeutic Effects in Type 2 Diabetes and Cancer vol.2018, pp.1942-0994, 2018, https://doi.org/10.1155/2018/9698258
  21. Mechanistic evaluation of phytochemicals in breast cancer remedy: current understanding and future perspectives vol.8, pp.52, 2018, https://doi.org/10.1039/C8RA04879G