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DOI QR Code

Ectopic Overexpression of COTE1 Promotes Cellular Invasion of Hepatocellular Carcinoma

  • Zhang, Hai (Liver Transplantation Center, First Affiliated Hospital of Nanjing Medical University, Key Laboratory of Living Donor Liver Transplantation, Ministry of Public Health) ;
  • Huang, Chang-Jun (Liver Transplantation Center, First Affiliated Hospital of Nanjing Medical University, Key Laboratory of Living Donor Liver Transplantation, Ministry of Public Health) ;
  • Tian, Yuan (Liver Transplantation Center, First Affiliated Hospital of Nanjing Medical University, Key Laboratory of Living Donor Liver Transplantation, Ministry of Public Health) ;
  • Wang, Yu-Ping (Shanghai-MOST Key Laboratory for Disease and Health Genomics, Chinese National Human Genome Center at Shanghai) ;
  • Han, Ze-Guang (Shanghai-MOST Key Laboratory for Disease and Health Genomics, Chinese National Human Genome Center at Shanghai) ;
  • Li, Xiang-Cheng (Liver Transplantation Center, First Affiliated Hospital of Nanjing Medical University, Key Laboratory of Living Donor Liver Transplantation, Ministry of Public Health)
  • 발행 : 2012.11.30

초록

Family with sequence similarity 189, member B (FAM189B), alias COTE1, a putative oncogene selected by microarray, for the first time was here found to be significantly up-regulated in hepatocellular carcinoma (HCC) specimens and HCC cell lines. mRNA expression of COTE1 in HCC samples and cell lines was detected by reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR, while protein expression of COTE1 in HCC tissues was assessed by immunohistochemistry. In addition, invasion of HCC cells was observed after overexpressing or silencing COTE1. In the total of 48 paired HCC specimens, compared with the adjacent non-cancer tissues, the expression of COTE1 was up-regulated in 31 (p<0.01). In HCC cell lines, COTE1 expression was significantly higher than in normal human adult liver (p<0.01). Overexpression of COTE1 enhanced HCC-derived LM6 and MHCC-L cellular invasion in vitro. In contrast, COTE1 knockdown via RNAi markedly suppressed these phenotypes, as documented in LM3 and MHCC-H HCC cells. Mechanistic analyses indicated that COTE1 could physically associate with WW domain oxidoreductase (WWOX), a tumor suppressor. COTE1 may be closely correlated with invasion of hepatocellular carcinoma (HCC) cells and thus may serve as an effective target for gene therapy.

키워드

참고문헌

  1. Adercal I, Moser CD, Veerasamy M, et al (2008). The JNK inhibitor SP600129 enhances apoptosis of HCC cells induced by the tumor suppressor WWOX. J Hepatol, 49, 373-83. https://doi.org/10.1016/j.jhep.2008.05.015
  2. Aqeilan RI, Donati V, Palamarchuk A, et al (2005). WW domain-containing proteins, WWOX and YAP, compete for interaction with ErbB-4 and modulate its transcriptional function. Cancer Res, 65, 6764-72. https://doi.org/10.1158/0008-5472.CAN-05-1150
  3. Aqeilan RI, Donati V, Gaudio E, et al (2007). Association of Wwox with ErbB4 in breast cancer. Cancer Res, 67, 9330-6. https://doi.org/10.1158/0008-5472.CAN-07-2147
  4. Aravalli RN, Steer CJ, and Cressman EN (2008). Molecular mechanisms of hepatocellular carcinoma. Hepatology, 48, 2047-63. https://doi.org/10.1002/hep.22580
  5. Abdeen SK, Salah Z, Maly B, et al (2011). Wwox inactivation enhances mammary tumorigenesis. Oncogene, 30, 3900-6. https://doi.org/10.1038/onc.2011.115
  6. Chang JY, He RY, Lin HP, et al (2010). Signaling from membrane receptors to tumor supressor WW domain-containing oxidoreductase. Exp Biol Med, 235, 796-804. https://doi.org/10.1258/ebm.2010.009351
  7. Chang NS, Doherty J, Ensign A (2003). JNK1 physically interacts with WW domain-cotaining oxidoreductase (WOX1) and inhibits WOX1-mediated apoptosis. J Biol Chem, 278, 9195-202. https://doi.org/10.1074/jbc.M208373200
  8. Chen ST, Chuang JI, Cheng CL, et al (2005). Light-induced retinal damage involves tyrosine 33 phosphorylation, mitochondrial and nuclear translocation of WW domaincontaining oxidoreductase in vivo. Neuroscience, 130, 397-407. https://doi.org/10.1016/j.neuroscience.2004.07.054
  9. Coleman WB. (2003) Mechanism of human hepatocarcinogenesis. Curr Mol Med, 3, 573-88. https://doi.org/10.2174/1566524033479546
  10. Del MS, Salah Z, Aqeilan RI (2009). WWOX: its genomics, partners, and functions. J Cell Biochem, 108, 737-45. https://doi.org/10.1002/jcb.22298
  11. Gourley C, Paige A, Taylor KJ, et al (2009). WWOX gene expression abolishes ovarian cancer tumorigenicity in vivo and decreases attachment to fibronectin via integrin alpha3. Cancer Res, 69, 4835-42. https://doi.org/10.1158/0008-5472.CAN-08-2974
  12. Guler G, Uner A, Guler N, et al (2003). The Fragile Genes FHIT and WWOX Are Inactivated Coordinately in Invasive Breast Carcinoma. Cancer, 100.
  13. Huan J, Zheng DL, Qin FS, et al (2010). Genetic and epigenetic silencing of SCARA5 may contribute to human hepatocellular carcinoma by activating FAK signaling. J Clin Invest, 120, 223-41. https://doi.org/10.1172/JCI38012
  14. Hong Q, Sze Ci, Lin SR, et al (2009). Complement C1q activates tumor suppressor WWOX to induce aopotosis in prostate cancer cells. PloS One, 4, e5755. https://doi.org/10.1371/journal.pone.0005755
  15. Kallin A, Johannessen LE, Cani PD, et al (2007). SREBP-1 regulates the expression of heme oxygenase 1 and the phosphatidylinositol-3 kinase regulatory subunit p55${\gamma}$. J Lipid Res, 48, 1628-36. https://doi.org/10.1194/jlr.M700136-JLR200
  16. Kurek KC, Del Mare S, Salah Z, et al (2010). Frequent attenuation of the WWOX tumor suppressor in osteosarcoma is associated with increased tumorigenicity and aberrant RUNX2 expression. Cancer Res, 70, 5577-86. https://doi.org/10.1158/0008-5472.CAN-09-4602
  17. Kim TM, Yim SH, Shin SH, et al (2008). Clinical implication of recurrent copy number alterations in hepatocellular carcinoma and putative oncogenes in recurrent gains on 1q. Int J Cancer, 123, 2808-15. https://doi.org/10.1002/ijc.23901
  18. Ludes-Meyers JH, Kil H, Bednarek AK, et al (2004). WWOX binds the specific proline-rich ligand PPXY: identification of candidate interanting proteins. Oncogene, 23, 5049-55. https://doi.org/10.1038/sj.onc.1207680
  19. Parkin DM, Bray F, Ferlay J, et al (2005). Global cancer statistics, 2002. CA Cancer J Clin, 55, 74-108. https://doi.org/10.3322/canjclin.55.2.74
  20. Salah Z, Aqeilan R and Huebner K (2010). WWOX gene and gene product tumor suppression through specific protein interactions. Future Oncol, 6, 249-59. https://doi.org/10.2217/fon.09.152
  21. Teng CC, Yang YT, Chen YC, et al (2012). Role of WWOX/ WOX1 in Alzheimer's disease pathology and in cell death signaling. Front Biosci (Elite Ed), 4, 1951-65.
  22. Wang JS, Huang T, Su J, et al (2001). Hepatocellular carcinoma and aflatoxin exposure in Zhuqing Village, Fusui County, People's Republic of China. Cancer Epidemiol Biomarkers Prev, 10, 143-6.
  23. Wong N, Chan A, Lee SW, et al (2003). Positional mapping for amplified DNA sequences on 1q21-q22 in hepatocellular carcinoma indicates candidate genes overexpression. J Hepatol, 38, 298-306.
  24. Wong N, Lam WC, Lai PB, et al (2001). Hypomethylation of chromosome 1 heterochromatin DNA correlates with q-arm copy gain in human hepatocellular carcinoma. Am J Pathol, 159, 465-71. https://doi.org/10.1016/S0002-9440(10)61718-X
  25. Winfield SL, Tayebi N, Martin BM, et al (1997). Identification of three additional genes contiguous to the glucocerebrosidase locus on chromosome 1q21: implications for Gaucher disease. Genome Res, 7, 1020-6. https://doi.org/10.1101/gr.7.10.1020
  26. Yu W, Bjorn Andersson, Worley KC, et al (1997). Large-scale concatenation cDNA sequencing. Genome Res, 7, 353-8. https://doi.org/10.1101/gr.7.4.353

피인용 문헌

  1. Targeting SHCBP1 Inhibits Cell Proliferation in Human Hepatocellular Carcinoma Cells vol.14, pp.10, 2013, https://doi.org/10.7314/APJCP.2013.14.10.5645
  2. Upregulation of the putative oncogene COTE1 contributes to human hepatocarcinogenesis through modulation of WWOX signaling vol.45, pp.2, 2014, https://doi.org/10.3892/ijo.2014.2482
  3. Transplantation of ATP7B–Transduced Bone Marrow Mesenchymal Stem Cells Decreases Copper Overload in Rats vol.9, pp.11, 2014, https://doi.org/10.1371/journal.pone.0111425
  4. Strategies of oncogenic microbes to deal with WW domain-containing oxidoreductase vol.240, pp.3, 2015, https://doi.org/10.1177/1535370214561957
  5. Cohort-wide deep whole genome sequencing and the allelic architecture of complex traits vol.9, pp.1, 2018, https://doi.org/10.1038/s41467-018-07070-8