DOI QR코드

DOI QR Code

Induction of Apoptosis by a Combination of Paclitaxel and Carboplatin in the Presence of Hyperthermia

  • Huang, Tao (Department of Orthopedics, the First Affiliated Hospital of China Medical University) ;
  • Gong, Wei-Hua (Beth Israel Deaconess Medical Center, Harvard Medical School) ;
  • Li, Xiu-Cheng (Department of Orthopedics, the First Affiliated Hospital of China Medical University) ;
  • Zou, Chun-Ping (Department of Orthopedics, the First Affiliated Hospital of China Medical University) ;
  • Jiang, Guang-Jian (Beth Israel Deaconess Medical Center, Harvard Medical School) ;
  • Li, Xu-Hui (Beth Israel Deaconess Medical Center, Harvard Medical School) ;
  • Feng, Dian-Peng (Department of Orthopedics, the First Affiliated Hospital of China Medical University)
  • 발행 : 2012.01.31

초록

Purpose: To study enhancing effects of paclitaxel in the thermochemotherapy of osteosarcoma cell lines and related mechanisms. Materials and Methods: Paclitaxel and carboplatin were used alone or jointly on OS732 cell lines in the presence of hyperthermia. Inhibition of proliferation was measured by MTT assay and cellular changes were assessed with inverted phase contrast and fluorescence microscopy. Apoptosis was analyzed with flow cytometry (FCM) and Fas expression by immunocytochemistry. Results: At $43^{\circ}C$, one hour after the application of 10ug/ml paclitaxel and $5{\mu}g/ml$ carboplatin on OS732 cells jointly, the survival rate was 15.8% which was significantly lower than with $10{\mu}g/ml$ paclitaxel (45.8%) and $5{\mu}g/ml$ carboplatin (47.7%) respectively (P<0.01). Moreover, changes of morphology and apoptotic rates indicated that the apoptosis-inducing effect of combined application was also much enhanced, as evident also regarding Fas expression. Conclusion: Paclitaxel is conducive to thermochemotherapy of osteosarcoma cell lines, possibly accomplished by up-regulation of Fas expression with induction of apoptosis.

키워드

참고문헌

  1. Andreou D, Bielack SS, Carrle D, et al (2011). The influence of tumor- and treatment-related factors on the development of local recurrence in osteosarcoma after adequate surgery. An analysis of 1355 patients treated on neoadjuvant Cooperative Osteosarcoma Study Group protocols. Ann Oncol, 22, 1228-35. https://doi.org/10.1093/annonc/mdq589
  2. Bacci G, Balladelli A, Palmerini E, et al (2008). Neoadjuvant chemotherapy for osteosarcoma of the extremities in preadolescentpatients: the Rizzoli Institute experience. J Pediatr Hematol Oncol, 30, 908-12. https://doi.org/10.1097/MPH.0b013e31817e4aee
  3. Bacci G, Rocca M, Salone M, et al (2008). High grade osteosarcoma of the extremities with lung metastases at presentation: treatment with neoadjuvant chemotherapy and simultaneous resection of primary andmetastatic lesions. J Surg Oncol, 98, 415-20. https://doi.org/10.1002/jso.21140
  4. Bakshandeh-Bath A, Stoltz AS, Homann N, et al (2009). Preclinical and clinical aspects of carboplatin and gemcitabine combined withwhole-body hyperthermia for pancreatic adenocarcinoma. Anticancer Research, 29, 3069-77.
  5. Debes A, Willers R, Gobel U, Wessalowski R (2005). Role of heat treatment in childhood cancers: distinct resistance profiles of solid tumor cell lines towards combined thermochemotherapy. Pediatr Blood Cancer, 45, 663-9. https://doi.org/10.1002/pbc.20266
  6. de Bree E, Theodoropoulos PA, Rosing H, et al (2006). Treatment of ovarian cancer using intraperitoneal chemotherapy with taxanes: from laboratory bench to bedside. Cancer Treat Rev, 32, 471-82. https://doi.org/10.1016/j.ctrv.2006.07.006
  7. de Bree E, Rosing H, Michalakis J, et al (2006). Intraperitoneal chemotherapy with taxanes for ovarian cancer with peritoneal dissemination. Eur J Surg Oncol, 32, 666-70. https://doi.org/10.1016/j.ejso.2006.03.008
  8. Drago-Ferrante R, Santulli A, Di Fiore R, et al (2008). Low doses of paclitaxel potently induce apoptosis in human retinoblastoma Y79 cells by up-regulating E2F1. Int J Oncol, 33, 677-87.
  9. Franke M, Hardes J, Helmke K, et al (2010). Solitary skeletal osteosarcoma recurrence. Findings from the CooperativeOsteosarcoma Study Group. Pediatr Blood Cancer, 56, 771-6.
  10. Fan QY, Ma BA, Zhou Y, Zhang MH, Hao XB (2003). Bone tumors of the extremities or pelvis treated by microwaveinduced hyperthermia. Clin Orthop Relat Res, 406, 165-75. https://doi.org/10.1097/00003086-200301000-00026
  11. Fiorillo A, DeRosa G, Giugliano F, et al (2009). Efficacy of pegylated lyposomal anthracyclines and of intraarterial carboplatin and doxorubicin combined with local hyperthermia in a case of malignant endovascular papillary angioendothelioma. Curr Drug Deliv, 6, 58-61. https://doi.org/10.2174/156720109787048212
  12. Goto T, Okuma T, Nakada I, Hozumi T, Kondo T (2007). Preoperative adjuvant therapy for primary malignant bone tumors. Gan To Kagaku Ryoho, 34, 1750-4.
  13. Huisman C, Ferreira CG, Bröker LE, et al (2002). Paclitaxel triggers cell death primarily via caspase-independent routes in the non-small cell lung cancer cell line NCI-H460. Clin Cancer Res, 8, 596-606.
  14. Kim HS, Lee YS, Kim DK (2009). Doxorubicin exerts cytotoxic effects through cell cycle arrest and Fas-mediated cell death. Pharmacology, 84, 300-9. https://doi.org/10.1159/000245937
  15. Liu B, Yang M, Li X, et al (2008). Enhanced efficiency of thermally targeted taxanes delivery in a human xenograft model of gastric cancer. J Pharm Sci, 97, 3170-81. https://doi.org/10.1002/jps.21194
  16. Li S, Zhou Y, Dong Y, CLEMENT IP (2007). Doxorubicin and selenium cooperatively induce fas signaling in the absence of Fas/Fas ligand interaction. Anticancer Res, 27, 3075-82.
  17. Michalakis J, Georgatos SD, de Bree E, et al (2007). Short-term exposure of cancer cells to micromolar doses of paclitaxel, with or without hyperthermia, induces long-term inhibition of cell proliferation and cell death in vitro. Ann Surg Oncol, 14, 1220-8. https://doi.org/10.1245/s10434-006-9305-4
  18. Mohamed F, Marchettini P, Stuart OA, Urano M, Sugarbaker PH (2003). Thermal enhancement of new chemotherapeutic agents at moderate hyperthermia. Ann Surg Oncol, 10, 463-8. https://doi.org/10.1245/ASO.2003.08.006
  19. Mishima K, Nariai Y, Yoshimura Y (2003). Carboplatin induces Fas (APO-1/CD95)-dependent apoptosis of human tongue carcinoma cells: sensitization for apoptosis by upregulation of FADD expression. Int J Cancer, 105, 593-600. https://doi.org/10.1002/ijc.11133
  20. Pires NMM, Eefting D, de Vries MR, Quax PHA, Wouter Jukema J (2007). Sirolimus and paclitaxel provoke different vascular pathological responses after local delivery in a murine model for restenosis on underlying atherosclerotic arteries. Heart, 93, 922-7. https://doi.org/10.1136/hrt.2006.102244
  21. Shido Y, Nishida Y, Suzuki Y, Kobayashi T, Ishiguro N (2010). Targeted hyperthermia using magnetite cationic liposomes and an alternating magnetic field in a mouse osteosarcoma model. J Bone Joint Surg Br, 92, 580-5. https://doi.org/10.1302/0301-620X.92B4.22814
  22. Stumm S, Meyer A, Lindner M, et al (2004). Paclitaxel treatment of breast cancer cell lines modulates Fas/Fas ligand expression and induces apoptosis which can be inhibited through the CD40 receptor. Oncology, 66, 101-11. https://doi.org/10.1159/000077435
  23. Sirichaisutdhikorn D, Suprasert P, Khunamornpong S (2009). Clinical outcome of the ovarian clear cell carcinoma compared to other epithelial ovarian cancers when treated with paclitaxel and carboplatin. Asian Pac J Cancer Prev, 10, 1041-5.
  24. Trieb K, Blahovec H, Kubista B (2007). Effects of hyperthermia on heat shock protein expression, alkaline phosphatase activity and proliferation in human osteosarcoma cells. Cell Biochem Funct, 25, 669-72. https://doi.org/10.1002/cbf.1371
  25. Yu DY, Matsuya Y, Zhao QL, et al (2007). Enhancement of hyperthermia-induced apoptosis by a new synthesized class of furan-fused tetracyclic compounds. Apoptosis, 12, 1523-32. https://doi.org/10.1007/s10495-007-0080-x
  26. Wang X, Gao XH, Li X, et al (2009). Local hyperthermia induces apoptosis of keratinocytes in both normal skin andcondyloma acuminata via different pathways. Apoptosis, 14, 721-8. https://doi.org/10.1007/s10495-009-0344-8
  27. Zoul Z, Filip S, Melichar B, et al (2004). Weekly paclitaxel combined with local hyperthermia in the therapy of breast cancer locally recurrent after mastectomy--a pilot experience. Onkologie, 27, 385-8. https://doi.org/10.1159/000079093
  28. Zhou JN, Huang XE, Ye Z, et al (2009). Weekly paclitaxel/ docetaxel combined with a platinum in the treatment of advanced non-small cell lung cancer: a study on efficacy, safety and pre-medication. Asian Pac J Cancer Prev, 10, 1147-50.

피인용 문헌

  1. Apoptosis-Induced Cell Death due to Oleanolic Acid in HaCaT Keratinocyte Cells -a Proof-of-Principle Approach for Chemopreventive Drug Development vol.13, pp.5, 2012, https://doi.org/10.7314/APJCP.2012.13.5.2015
  2. Cytotoxicity, Apoptosis Induction and Anti-Metastatic Potential of Oroxylum indicum in Human Breast Cancer Cells vol.13, pp.6, 2012, https://doi.org/10.7314/APJCP.2012.13.6.2729