Journal of Chest Surgery

The Korean Society for Thoracic and Cardiovascular Surgery (대한심장혈관흉부외과학회)
권호 표지
DOI QR코드

DOI QR Code

Histologic Characteristics and Mechanical Properties of Bovine Pericardium Treated with Decellularization and ${\alpha}$-Galactosidase: A Comparative Study

  • Min, Byoung-Ju (Department of Thoracic and Cardiovascular Surgery, Seoul National University College of Medicine) ;
  • Kim, Yong Jin (Department of Thoracic and Cardiovascular Surgery, Seoul National University Hospital, Seoul National University College of Medicine) ;
  • Choi, Jae-Woong (Department of Thoracic and Cardiovascular Surgery, Seoul National University Hospital, Seoul National University College of Medicine) ;
  • Choi, Sun Young (Department of Thoracic and Cardiovascular Surgery, Seoul National University College of Medicine) ;
  • Kim, Soo Hwan (Department of Thoracic and Cardiovascular Surgery, Seoul National University College of Medicine) ;
  • Lim, Hong-Gook (Department of Thoracic and Cardiovascular Surgery, Seoul National University Hospital, Seoul National University College of Medicine)
  • Received : 2010.09.15
  • Accepted : 2011.02.08
  • Published : 2012.12.05
Download PDF
⟨ Previous Next ⟩

Abstract

Background: Bioprostheses for cardiovascular surgery have limitations in their use following as calicification. ${\alpha}$-galactosidase epitope is known as a stimulant of immune response and then shows a progressing calcification. The objective of this study was to evaluate histologic characteristics and mechanical properties of decellularization and treated with ${\alpha}$-galactosidase. Materials and Methods: Bovine pericardial tissues were allocated into three groups: fixation only with glutaraldehyde, decellularization with sodium dodesyl sulfate and decellularization plus treatment with ${\alpha}$-galactosidase. We confirmed immunohistological characteristics and mechanical properties as fatigue test, permeability test, compliance test, tensile strength (strain) test and thermal stability test. Results: Decellularization and elimination of ${\alpha}$-gal were confirmed through immunohistologic findings. Decellularization had decreased mechanical properties compared to fixation only group in permeability (before fatigue test p=0.02, after fatigue test p=0.034), compliance (after fatigue test p=0.041), and tensile strength test (p=0.00). The group of decellularization plus treatment with ${\alpha}$-galactosidase had less desirable mechanical properties than the group of decellularization in concerns of permeability (before fatigue test p=0.043) and strain test (p=0.001). Conclusion: Favorable decellularization and elimination of ${\alpha}$-gal were obtained in this study through immunohistologic findings. However, those treatment including decellularization and elimination of ${\alpha}$-gal implied the decreased mechanical properties in specific ways. We need more study to complete appropriate bioprosthesis with decellularization and elimination of ${\alpha}$-gal including favorable mechanical properties too.

Keywords

References

  1. Jamieson WR, Rosado LJ, Munro AI, et al. Carpentier- Edwards standard porcine bioprosthesis: primary tissue failure (structural valve deterioration) by age groups. Ann Thorac Surg 1988;46:155-62. https://doi.org/10.1016/S0003-4975(10)65888-2
  2. David TE, Ivanov J. Is degenerative calcification of the native aortic valve similar to calcification of bioprosthetic heart valves? J Thorac Cardiovasc Surg 2003;126:939-41. https://doi.org/10.1016/S0022-5223(03)00731-1
  3. Human P, Zilla P. Characterization of the immune response to valve bioprostheses and its role in primary tissue failure. Ann Thorac Surg 2001;71(5 Suppl):S385-8. https://doi.org/10.1016/S0003-4975(01)02492-4
  4. Konakci KZ, Bohle B, Blumer R, et al. Alpha-Gal on bioprostheses: xenograft immune response in cardiac surgery. Eur J Clin Invest 2005;35:17-23. https://doi.org/10.1111/j.1365-2362.2005.01441.x
  5. Galili U, Clark MR, Shohet SB, Buehler J, Macher BA. Evolutionary relationship between the natural anti-Gal antibody and the Gal alpha 1----3Gal epitope in primates. Proc Natl Acad Sci U S A 1987;84:1369-73. https://doi.org/10.1073/pnas.84.5.1369
  6. Galili U, Shohet SB, Kobrin E, Stults CL, Macher BA. Man, apes, and Old World monkeys differ from other mammals in the expression of alpha-galactosyl epitopes on nucleated cells. J Biol Chem 1988;263:17755-62.
  7. Galili U, Andrews P. Suppression of ${\alpha}$-galactosyl epitopes synthesis and production of the natural anti-Gal antibody: a major evolutionary event in ancestral old world primates. J Hum Evol 1995;29:433-42. https://doi.org/10.1006/jhev.1995.1067
  8. Gratzer PF, Pereira CA, Lee JM. Solvent environment modulates effects of glutaraldehyde crosslinking on tissue-derived biomaterials. J Biomed Mater Res 1996;31:533-43. https://doi.org/10.1002/(SICI)1097-4636(199608)31:4<533::AID-JBM14>3.0.CO;2-H
  9. Zilla P, Weissenstein C, Human P, Dower T, von Oppell UO. High glutaraldehyde concentrations mitigate bioprosthetic root calcification in the sheep model. Ann Thorac Surg 2000;70:2091-5. https://doi.org/10.1016/S0003-4975(00)02011-7
  10. Pathak CP, Adams AK, Simpson T, Phillips RE Jr, Moore MA. Treatment of bioprosthetic heart valve tissue with long chain alcohol solution to lower calcification potential. J Biomed Mater Res A 2004;69:140-4.
  11. Grauss RW, Hazekamp MG, van Vliet S, Gittenberger-de Groot AC, DeRuiter MC. Decellularization of rat aortic valve allografts reduces leaflet destruction and extracellular matrix remodeling. J Thorac Cardiovasc Surg 2003;126:2003-10. https://doi.org/10.1016/S0022-5223(03)00956-5
  12. Sung SC, Kim YJ, Choi SY, Park JE, Kim KH, Kim WH. A study on an effective decellularization technique for a xenograft cardiac valve: the effect of osmotic treatment with hypotonic solution. Korean J Thorac Cardiovasc Surg 2008;41: 679-86.
  13. Phelps CJ, Koike C, Vaught TD, et al. Production of alpha 1,3-galactosyltransferase-deficient pigs. Science 2003;299:411-4. https://doi.org/10.1126/science.1078942
  14. Dai Y, Vaught TD, Boone J, et al. Targeted disruption of the alpha1,3-galactosyltransferase gene in cloned pigs. Nat Biotechnol 2002;20:251-5. https://doi.org/10.1038/nbt0302-251
  15. Luo Y, Wen J, Luo C, Cummings RD, Cooper DK. Pig xenogeneic antigen modification with green coffee bean alpha- galactosidase. Xenotransplantation 1999;6:238-48. https://doi.org/10.1034/j.1399-3089.1999.00035.x
  16. Goldstein IJ, Blake DA, Ebisu S, Williams TJ, Murphy LA. Carbohydrate binding studies on the Bandeiraea simplicifolia I isolectins. Lectins which are mono-, di-, tri-, and tetravalent for N-acetyl-D-galactosamine. J Biol Chem 1981;256:3890-3.
  17. Goldstein IJ, Winter HG. The Griffonia simplicifolia I-B4 isolectin: a probe for alpha-D-galactosyl end groups. SubcellBiochem 1999;32:127-41.
  18. Kirkeby S, Moe D. Binding of Griffonia simplicifolia 1 isolectin B4 (GS1 B4) to alpha-galactose antigens. Immunol Cell Biol 2001;79:121-7. https://doi.org/10.1046/j.1440-1711.2001.00992.x
  19. Kirkeby S, Andre S, Gabius HJ. Solid phase measurements of antibody and lectin binding to xenogenic carbohydrate antigens. Clin Biochem 2004;37:36-41. https://doi.org/10.1016/S0009-9120(03)00120-6
  20. LaVecchio JA, Dunne AD, Edge AS. Enzymatic removal of alpha-galactosyl epitopes from porcine endothelial cells diminishes the cytotoxic effect of natural antibodies. Transplantation 1995;60:841-7. https://doi.org/10.1097/00007890-199510270-00014

Cited by

  1. In VivoEfficacy of Alpha-Galactosidase as Possible Promise for Prolonged Durability of Bioprosthetic Heart Valve Using Alpha1,3-Galactosyltransferase Knockout Mouse vol.19, pp.21, 2012, https://doi.org/10.1089/ten.tea.2013.0062
  2. Combining tissue repair and tissue engineering; bioactivating implantable cell-free vascular scaffolds vol.100, pp.23, 2014, https://doi.org/10.1136/heartjnl-2014-306092
  3. In vivo efficacy for novel combined anticalcification treatment of glutaraldehyde-fixed cardiac xenograft using humanized mice vol.29, pp.7, 2012, https://doi.org/10.1177/0885328214552710
  4. Decellularized inner body membranes for tissue engineering: A review vol.31, pp.10, 2012, https://doi.org/10.1080/09205063.2020.1751523
  5. Decellularized xenografts in regenerative medicine: From processing to clinical application vol.28, pp.4, 2012, https://doi.org/10.1111/xen.12683