The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Effect of $Agrimonia$ $pilosa$ $Ledeb$ Extract on the Antinociception and Mechanisms in Mouse

  • Park, Soo-Hyun (Institute of Natural Medicine, College of Medicine, Hallym University) ;
  • Sim, Yun-Beom (Institute of Natural Medicine, College of Medicine, Hallym University) ;
  • Kang, Yu-Jung (Institute of Natural Medicine, College of Medicine, Hallym University) ;
  • Lee, Jin-Koo (Department of Pharmacology, College of Medicine, Translational Research Center, Institute of Bio-Science and Technology, Dankook University) ;
  • Lim, Soon-Sung (Institute of Natural Medicine, College of Medicine, Hallym University) ;
  • Suh, Hong-Won (Institute of Natural Medicine, College of Medicine, Hallym University)
  • Received : 2011.01.07
  • Accepted : 2012.02.21
  • Published : 2012.04.30
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Abstract

In the present study, the antinociceptive profiles of $Agrimonia$ $pilosa$ $Ledeb$ extract were examined in ICR mice. $Agrimonia$ $pilosa$ $Ledeb$ extract administered orally (200 mg/kg) showed an antinociceptive effect as measured by the tail-flick and hot-plate tests. In addition, $Agrimonia$ $pilosa$ $Ledeb$ extract attenuated the writhing numbers in the acetic acid-induced writhing test. Furthermore, the cumulative nociceptive response time for intrathecal (i.t.) injection of substance P (0.7 ${\mu}g$) was diminished by $Agrimonia$ $pilosa$ $Ledeb$ extract. Intraperitoneal (i.p.) pretreatment with yohimbine (${\alpha}_2$-adrenergic receptor antagonist) attenuated antinociceptive effect induced by $Agrimonia$ $pilosa$ $Ledeb$ extract in the writhing test. However, naloxone (opioid receptor antagonist) or methysergide (5-HT serotonergic receptor antagonist) did not affect antinociception induced by $Agrimonia$ $pilosa$ $Ledeb$ extract in the writhing test. Our results suggest that $Agrimonia$ $pilosa$ $Ledeb$ extract shows an antinociceptive property in various pain models. Furthermore, this antinociceptive effect of $Agrimonia$ $pilosa$ $Ledeb$ extract may be mediated by ${\alpha}_2$-adrenergic receptor, but not opioidergic and serotonergic receptors.

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References

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