Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effect of Vigna angularis Ethanol Extract on Papain-induced Arthritis in Mice

팥 에탄올 추출물의 Papain 유도 관절염 마우스에서의 항 골관절염 효과

  • Jeoung, See Hwa (Natural Bio R&D Center, Sun Bio Tech.) ;
  • Kim, Seung Hyung (Department of Oriental Medicine, College of Oriental Medicine, Dajeon University) ;
  • Kim, Hyun Kyu (Natural Bio R&D Center, Sun Bio Tech.) ;
  • Yun, Bo Ra (Natural Bio R&D Center, Sun Bio Tech.) ;
  • Lee, Hee Woong (Natural Bio R&D Center, Sun Bio Tech.) ;
  • Lim, Ju Hwan (Natural Bio R&D Center, Sun Bio Tech.) ;
  • Rho, Mun Chual (Bioindustrial Process Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Dong Hee (Department of Oriental Medicine, College of Oriental Medicine, Dajeon University)
  • 정시화 (선바이오텍 천연바이오 신소재 연구소) ;
  • 김승형 (대전대학교 한의과대학 병리학교실) ;
  • 김현규 (선바이오텍 천연바이오 신소재 연구소) ;
  • 윤보라 (선바이오텍 천연바이오 신소재 연구소) ;
  • 이희웅 (선바이오텍 천연바이오 신소재 연구소) ;
  • 임주환 (선바이오텍 천연바이오 신소재 연구소) ;
  • 노문철 (한국생명공학연구원 생물산업공정 연구센터) ;
  • 김동희 (대전대학교 한의과대학 병리학교실)
  • Received : 2012.09.24
  • Accepted : 2012.10.16
  • Published : 2012.10.25
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Abstract

The purpose of this study is to examine the effect of Vigna angularis ethanol extract (VA-E) on papain induced arthritis in C57BL/10 mice. The C57BL/10 mice were injected with papain and oral administration of Vigna angularis ethanol extract (VA-E) at different doses (100 and 200 mg/kg) once a day for 42 days and compared with untreated mice. Histological examination and the measurement of inflammatory cytokine release were performed. The results showed that comparing with untreated mice, the treatment with Vigna angularis ethanol extract (VA-E) decreased the pathological changes of knee joint tissue. The level of inflammatory cytokine secreted from the tissues, such as IL-$1{\beta}$ (interleukin-$1{\beta}$), IL-6 (interleukin-6), TNF-${\alpha}$ (Tumor Necrosis Factor-${\alpha}$), were decreased. These results were confirmed by the quantification of cartilage volume using micro CT scanning. Consequently, Vigna angularis ethanol extract (VA-E) has a therapeutic potential in inflammatory joint diseases such as osteoarthritis.

Keywords

Acknowledgement

Supported by : 특구진흥재단

References

  1. 질병관리본부 만성병조사팀. 2005 건강행태 및 만성질환 통계 자료집. 서울 : 보건복지부. pp 133-134, 2006.
  2. 대한정형외과학회. 정형외과. 6판. 서울, 최신의학사, pp 195-201, 2005. pp 226, 229-231, 2006.
  3. 李今庸主編. 新編黃帝內經綱目. 上海: 上海科學技術出版社, p 262, 1988.
  4. 대한병리학회. 병리학 제5판. 서울, 고문사 pp 58-59, 2003.
  5. American College of Rheumatology Subcom mittee on Osteoarthritis Guidelines. Recommendations for the medical management of osteoarthritis of the hip and knee. Arthritis & Rheumatism. 43(9):1905-1915, 2000. https://doi.org/10.1002/1529-0131(200009)43:9<1905::AID-ANR1>3.0.CO;2-P
  6. Rho, C.W., Son, S.Y., Hong, S.T., Lee, K.H., Ryu, I.M. Agronomic characters of Korean adzuki beans (Vigna angularis (Willd.) Ohwi &Ohashi). Korean J. Plant Res. 16: 147-154, 2003.
  7. Chang, K.Y. Han, K.S., Park, J.C. Studies on the selection in adzuki bean breeding. III. Phenotypic and genotypic correlations among some characte rs in the population of adzuki bean varieties. Res. Bull. Chinju Agric. College, Korea. 7: 39-44, 1968.
  8. Chang, H.G. Information on Food for the Health of Modern People. Shinkwang Publishing Co., Seoul, Korea. pp 46-50, 1999.
  9. 李時珍. 本草綱目. 北京. 人民衛生出版社, pp 508-512, 833-837, 1456-1457, 1982.
  10. Choi, S.Y., Jeong, Y.J., Lee, S.J., Chi, O.H., Chegal, S.A. Food and Health for Modern People. Dongmyungsa, Seoul, Korea. pp 244-246, 2002.
  11. Yoshida, K., Sato, Y., Okuno, R., Kameda, K., Isobe, M., Kondo, T. Structural analysis and measurement of anthocyanin from colored seed coats of Vigna, Phaseolus, and Glycine Lugumes Biosci. Biotech. Bioch. 60: 589-593, 1996. https://doi.org/10.1271/bbb.60.589
  12. Ariga, T., Koshiyama, I., Fukushima, D. Antioxidative properties of procyanidins B-1 and B-3 from azuki beans in aqueous systems. Agr. Biol. Chem. 52: 2717-2722, 1988. https://doi.org/10.1271/bbb1961.52.2717
  13. Koide, T., Hashimoto, Y., Kamei, H., Kojima, T., Hasegawa, M., Terabe, K. Antitumor effect of anthocyanin fractions extracted from red soybeans and red beans in vitro and in vivo. Cancer Biother. Radio. 12: 277-280, 1997.
  14. Ariga, T., Hamano, M. Radical scavenging action and its mode in procyanidins B-1 and B-3 from azuki beans to peroxyl radicals. Agricultural Biology and Chemistry. 54: 2499-2504, 1990. https://doi.org/10.1271/bbb1961.54.2499
  15. Kawai, T., Akira, S. Signaling to NF-kB by Toll-like receptors. Trends in Molecular Medicine. 13(11):460-469, 2007. https://doi.org/10.1016/j.molmed.2007.09.002
  16. Piscaer, T.M., Waarsing, J.H., Kops, N., Pavljasevic, P., Verhaar, J.A., Van Osch, G.J., and Weinans, H. In vivo imaging of cartilage degeneration using mCT-arthrography. Osteoarthritis and Cartilage. 16(9):1011-1017, 2008. https://doi.org/10.1016/j.joca.2008.01.012
  17. 보건복지부, 보건복지통계연보. 1995.
  18. 대한 병리학회, 병리학, 고문사, 서울. pp 1166-1177, 1994.
  19. Sarzi-Puttini, P., Cimmino, M.A., Scarpa, R., Caporali, R., Parazzini, F., Zaninelli,. A., Atzeni, F., Canesi, B. Osteoarthritis ; an overview of the disease and its treatment strategies, semin Arthritis Rheum. 35: 1-10, 2005.
  20. The Korean Orthopaedic Association. orthopedics. 5th ed. seoul : cholsin medicine publisher. pp 109-131, 157-174, 176, 2000.
  21. 허정은 외 7명. 퇴행성관절염 치료제 개발을 위한 수종의 한약재활성 검색 및 기전 연구. J Korean Oriental Med. 27(1):229-239, 2006.
  22. 박동수. 봉독약침이 Monosodium Iodoacetate로 유발된 골관절염 통증 모델에서 중추신경내 NOS, C-fos, Serotonin, Substance P 발현에 미치는 영향, 세명대학교 대학원, 2007.
  23. 이경무. 三氣飮이 흰쥐의 Monosodium Iodoacetate 유발 골관절염에 미치는 영향, 세명대학교 대학원, 2008.
  24. 박원태. 大防風湯이 Monosodium Iodoacetate로 유발된 흰쥐의 골관절염에 미치는 영향, 세명대학교 대학원, 2008.
  25. 이승헌. 秦구.威靈仙.夏枯草 복합방이 monosodium iodoacetate로 유발된 흰쥐의 골관절염에 미치는 영향, 세명 대학교 대학원, 2007.
  26. 이정민. Papain으로 유도된 골관절염 생쥐 모델에서 芍藥甘草附子湯의 항골관절염 효능에 관한 연구, 대전대학교 대학원, 2012.
  27. 오찬호 역. 신면역학입문. 서울, 지구문화사, pp 63, 118, 120-128, 175-87, 1997.
  28. 강재성 외. 세포분자면역학 5판. 서울, 범문사, pp 243-253, 261-269, 423, 2004.
  29. 김미화 외 7명. 적소두 추출물이 톨유사수용체 활성 및 염증 유발 사이토카인의 생성에 미치는 영향. Korean J. Oriental Physiology & Pathology. 26(4):511-518. 2012.