한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제41권5호
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  • Pages.584-590
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  • 2012
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  • 1226-3311(pISSN)
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  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
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국화과 추출물의 암세포 증식 억제 효과

induction of Apoptosis in Human Cancer Cells with Compositae Extracts

  • An, In-Jung (Dept. of Companion and Laboratory Animal Science, Kongju National University) ;
  • Kwon, Jung-Ki (Dept. of Companion and Laboratory Animal Science, Kongju National University) ;
  • Lee, Jin-Seok (Dept. of Companion and Laboratory Animal Science, Kongju National University) ;
  • Park, Ha-Seung (Yesan Chrysanthemum Experiment Station) ;
  • Kim, Dong-Chan (Yesan Chrysanthemum Experiment Station) ;
  • Choi, Byung-Jun (Yesan Chrysanthemum Experiment Station) ;
  • Lee, Kyu-Min (Dept. of Plant Science and Technology, Sangmyung University) ;
  • Park, Youg-Jin (Legume Bio-Resource Center of Green Manure (LBRCGM), Kongju National University) ;
  • Jung, Ji-Youn (Dept. of Companion and Laboratory Animal Science, Kongju National University)
  • 투고 : 2012.02.09
  • 심사 : 2012.04.03
  • 발행 : 2012.05.31
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초록

국화과 꽃은 우리나라에서 전통적으로 항염증과 항산화 치료에 사용되었다. 본 연구에서는 국화과 추출물이 인간 위암세포 AGS, 인간 유방암세포 MDA-MB-231과 SK-BR-3 암세포에서 성장을 억제하고 세포자멸사를 유발하는지 확인하였다. AGS, MDA-MB-231 그리고 SK-BR-3 암세포의 성장을 MTT로 측정하였다. 14종의 국화과 추출물을 24시간 동안 50, 100, 200 ${\mu}g/mL$의 농도로 처치하였다. 한라구절초 전초, 포천구절초 전초, 삼잎국화 지하부, 낙동구절초 전초, 산국 전초 그리고 해국 꽃 추출물에서 암세포의 성장을 농도 의존적으로 억제시켰다. 우리나라 여성에서 가장 많이 발생하는 유방암세포인 MDA-MB-231 암세포에서 세포자멸사를 확인하기 위해 DAPI 염색을 수행하였다. MTT assay에서 암세포를 억제시킨 6종의 국화과 추출물중 3종인 한라구절초 전초, 포천구절초 전초, 삼잎국화 지하부 추출물을 처치한 세포에서 핵의 응축이 농도 의존적으로 존재함을 형광현미경으로 확인하였다(${\times}200$). 세포자멸사에 관련된 단백질의 발현을 알아보기 위해서 western blot으로 확인하였다. 한라구절초 전초, 포천구절초 전초, 삼잎국화 지하부 추출물을 25, 50 ${\mu}g/mL$의 농도로 24시간 동안 MDA-MB-231 암세포 처치 후 cell lysate를 얻어 Bcl-2, Bax 그리고 p53의 변화를 관찰하였다. 한라구절초 전초, 포천구절초 전초 그리고 삼잎국화 지하부에서 anti-apoptotic 분자인 Bcl-2 단백질은 감소하고 반대로 pro-apoptotic 분자인 Bax와 p53 단백질은 증가하였다. 결과적으로 한라구절초 전초, 포천구절초 전초 그리고 삼잎국화 지하부 추출물은 유방암 세포의 성장을 억제하고 apoptosis를 유발시키므로 암예방제나 치료제로 개발될 수 있을 것으로 사료된다.

Dried $Compositae$ flowers have traditionally been used for the treatment of anti-inflammatory and anti-oxidative stress in Korea. This paper investigates the effects of $Compositae$ extracts on the inhibition of proliferation and apoptosis of human gastric cancer AGS cells, human breast cancer MDA-MB-231 cells, and SK-BR-3 cells. The proliferation of AGS cells, MDA-MB-231 cells, and SK-BR-3 cells were determined by MTT assay. Several $Compositae$ extracts inhibited proliferation of AGS cells, MDA-MB-231 cells, and SK-BR-3 cells in a dose-dependent manner. To assess the apoptosis of $Compositae$ extracts, the nuclei of MDA-MB-231 cells were stained with DAPI. The presence of chromatin condensation in the $Compositae$ extract-treated cells was detected on a fluorescent microscope (${\times}200$). We conducted Western blot analysis of changes in Bcl-2, Bax, and p53 protein expression levels. Apoptosis by $Chrysanthemum$ $zawadskii$ subsp. coreanum, $Chrysanthemum$ $zawadskii$ var. $tenuisectum$ and $Rudbeckia$ $laciniata$ var. $hortensis$ treatment created a decrease in Bcl-2 expression, whereas the expression of Bax and p53 were increased. These results indicate that $Chrysanthemum$ $zawadskii$ $subsp.$ $coreanum$, $Chrysanthemum$ $zawadskii$ var. $tenuisectum$ and $Rudbeckia$ $laciniata$ var. $hortensis$ inhibit breast cancer cell growth through the induction of apoptosis.

키워드

참고문헌

  1. Lee SE, Hwang HJ, Ha JS, Jeong HS, Kim JH. 2003. Screening of medical plant extracts for antioxidant activity. Life Sci 73: 167-179. https://doi.org/10.1016/S0024-3205(03)00259-5
  2. Sun T, Tanumihardjo SA. 2007. An integrated approach to evaluate food antioxidant capacity. J Food Sci 72: 159-165.
  3. Choi SH, Im SI, Bae JE. 2006. Analysis of aroma components from flower tea of German chamomile and Chrysantehmum boreale Makino. Korea J Food Cookery Sci 22: 768-773.
  4. Kang JR, Lee MK, Kang SM. 2008. Anti-oxidant property and tyrosinase inhibition activity of various extracts from plants in compositae plants. J Korea Soc Appl Biol Chem 51: 321-328. https://doi.org/10.3839/jksabc.2008.056
  5. Park NY, Kown JH, Kim HK. 1998. Optimization of extraction conditions for ethanol extracts from Chrysanthemum morifolium by response surface methodology. Korean J Food Sci Technol 31: 1189-1196.
  6. Nam SH, Yang MS. 1995. Antibacterial activities of extracts from Chryasnthemum boreale M. J Agric Food Chem 38: 269-272.
  7. Nam SH, Yang MS. 1995. Isolation of cytotoxic substances of extracts from Chrysanthemum boreale M. J Agric Food Chem 38: 273-277.
  8. Woo JH, Lee CH. 2008. Effects of harvest date on antioxidant of Dendranthema zawadskii var. latilobum (Maxim.) Kitam and D. zawadskii var. yezoense (Maek) Y.M. Lee & H.J. Choi. Korean J Plant Res 21: 128-133.
  9. Son JC, Park NK, Hur HS, Bang MH, Baek NI. 2000. Examination and isolation of natural antioxidants from Korean medicinal plants. Korea J Med Crop Sci 8: 94-101.
  10. Korea National Statistic Office. 2005. The Korean cause of death.
  11. Chen CN, Huang HH, Wu CL, Lin PC, Hsu JT, Hsieh HP, Chuang SE, Lai GM. 2007. Isocostunolide, a sesquiterpene lactone, induces mitochondrial membrane depolarization and caspase-dependent apoptosis in human melanoma cells. Cancer Lett 246: 237-252. https://doi.org/10.1016/j.canlet.2006.03.004
  12. Hajra KM, Liu JR. 2004. Apoptosome dysfunction in human cancer. Apoptosis 9: 691-704. https://doi.org/10.1023/B:APPT.0000045786.98031.1d
  13. Reed J. 1994. Bcl-2 and regulation of programmed cell death. J Cell Biol 124: 1-6. https://doi.org/10.1083/jcb.124.1.1
  14. Kim EJ, Lee YJ, Shin HK, Park JH. 2005. Induction of apoptosis by the aqueous extract of Rubuscoreanum in HT-29 human colon cancer cells. Nutrition 21: 1141-1148. https://doi.org/10.1016/j.nut.2005.02.012
  15. Jung JI, Lim SS, Choi HJ, Cho HJ, Shin HK, Kim EJ, Chung WY, Park KK, Park JH. 2006. Iosliquiritigenin induces apoptosis by depolarizing mitochondrial membranes in prostate cancer cells. J Nutr Biochem 17: 689-696. https://doi.org/10.1016/j.jnutbio.2005.11.006
  16. Hu CQ, Chen K, Shi Q, Kilkuskie RE, Cheng YC, Lee KH. 1994. Anti-AIDS agents, 10. Acacetin-7-O-beta-D-galactopyranoside, an anti-HIV principle from Chrysanthemum morifolium and a structure-activity correlation with some related flavonoids. J Nat Prod 57: 42-51. https://doi.org/10.1021/np50103a006
  17. Sassi AB, Harzallah-Skhiri F, Bourgougnon N, Aouni M. 2008. Antimicrobial activities of four Tunisian Chrysanthemum species. Ind J Med Res 127: 183-192.
  18. Marongiu B, Piras A, Porcedda S, Tuveri E, Laconi S, Deidda D, Maxia A. 2009. Chemical and biological comparisons on supercritical extracts of Tanacetum cinerariifolium (Trevir) Sch. Bip. with three related species of chrysanthemums of Sardinia (Italy). Nat Prod Res 23: 190-199. https://doi.org/10.1080/14786410801946221
  19. Yu JS, Hwang IG, Woo KS, Chang YD, Lee CH, Jeong JH, Jeong HS. 2008. Physicochemical characteristics of Chrysanthemum indicum L. flower tea according to different pan-firing times. Korean J Food Sci Technol 40: 297-302.
  20. Cai HF. 2007. The research progression of Flos Chrysanthemum indicum on chemical constituent and medical application. Zhongguo Yiliao Quanyan 2: 118-120.
  21. Jin SR, Zhu BD, Qin XH. 2005. The effect of Chrysanthemum indicum on SMMC7721, PC3 and HL60 cell lines. Zhongyao Yaoli Yu Linchuang 21: 39-41.
  22. Wu DH, Yang LW, Su WW. 2004. The research progression of Chrysanthemum indicum on chemical constituent and pharmacology. Zhongyaocai 27: 142-144.
  23. Evans VG. 1993. Multiple pathways to apoptosis. Cell Biol Int 17: 461-476. https://doi.org/10.1006/cbir.1993.1087
  24. Park SY, Kim EJ, Lim DY, Kim JS, Lim SS, Shin HK, Yoon JH. 2008. Inhibitory effect of the hexane extract of Saussurea lappa on the growth of LNCaP human prostate cancer cells. J Korean Soc Food Sci Nutr 37: 8-15. https://doi.org/10.3746/jkfn.2008.37.1.8
  25. Banasiak KJ, Xia Y, Haddad GG. 2000. Mechanism underlying hypoxia-induced neuronal apoptosis. Prog Neurobiol 62: 215-249. https://doi.org/10.1016/S0301-0082(00)00011-3
  26. Amundson SA, Myers TG, Fornace AJ. 1998. Roles for p53 in growth arrest and apoptosis: putting on the brakes after genotoxic stress. Oncogene 17: 3287-3299. https://doi.org/10.1038/sj.onc.1202576
  27. Kim EJ, Park SY, Hong J, Shin M, Lim SS, Shin HK, Park JHY. 2007. Inhibitory effect of the methanolic extract of Symphyocladia latiuscula on the growth of HT-29 human colon cancer cells. J Korean Soc Food Sci Nutr 36: 431-438. https://doi.org/10.3746/jkfn.2007.36.4.431
  28. Donovan M, Cotter TG. 2004. Control of mitochondrial integrity by Bcl-2 family members and caspase-independent cell death. Biochim Biophys Acta 1644: 133-147. https://doi.org/10.1016/j.bbamcr.2003.08.011
  29. Kim EJ, Park HS, Lim SS, Kim JS, Shin HK, Yoon JH. 2008. Effect of the hexane extract of Saussurea lappa on the growth of HT-29 human colon cancer cells. Korean J Food Sci Techinol 40: 207-214.

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