Antioxidative and Anti-inflammatory Effect of Ethanol Extract from Duchesnea chrysantha

사매 에탄올 추출물의 항산화 및 항염증 효과

  • Lee, Deok-Jae (School of Alternative Medicine & Health Science, College of Alternative Medicine, Jeonju University) ;
  • Jeon, In-Hwa (School of Alternative Medicine & Health Science, College of Alternative Medicine, Jeonju University) ;
  • Kim, Hyeon-Soo (School of Alternative Medicine & Health Science, College of Alternative Medicine, Jeonju University) ;
  • Cho, Il-Young (School of Alternative Medicine & Health Science, College of Alternative Medicine, Jeonju University) ;
  • Jang, Seon-Il (School of Alternative Medicine & Health Science, College of Alternative Medicine, Jeonju University)
  • 이덕재 (전주대학교 대체의학대학 대체건강관리학부) ;
  • 전인화 (전주대학교 대체의학대학 대체건강관리학부) ;
  • 김현수 (전주대학교 대체의학대학 대체건강관리학부) ;
  • 조일영 (전주대학교 대체의학대학 대체건강관리학부) ;
  • 장선일 (전주대학교 대체의학대학 대체건강관리학부)
  • Received : 2012.01.06
  • Accepted : 2012.02.13
  • Published : 2012.02.25

Abstract

Oxidative stress has been implicated in cutaneous damage in various inflammatory skin diseases, including atopic dermatitis. The present study was undertaken to investigate the antioxidative and anti-inflammatory activities of the extract of Duchesnea chrysantha (DCE). DEC was prepared by extracting with 80% ethanol. Total flavonoids and polyphenols were measured by a colorimetric assay. The free radical scavenging activity of the extract was analyzed by the DPPH (1,1-diphenyl-2-picryl hydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) and Griess reagent assay. An oxidative product of nitric oxide (NO), was measured in the culture medium by the Griess reaction. The level of prostaglandin $E_2$ ($PGE_2$) was measured by enzyme-linked immunosorbent assay. The expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were measured by Western blot analysis. Total flavonoid and polyphenol contents of DCE were included $24.73{\pm}0.45$ and $178.77{\pm}2.65$, respectively. DCE significantly increased electron donating ability (DPPH), nitrite scavenging (NO) and ABTS reducing activity in dose dependant. We investigated the anti-inflammatory effects of DCE on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. DCE significantly suppressed NO and prdstaglandin $E_2$ ($PGE_2$) in dose dependant. Furthermore, the levels of iNOS and COX-2 protein expressions were markedly suppressed by the treatment with DCE in a dose dependent manner. These results suggest that DEC may has value as natural product with its high quality functional components, antioxidative and anti-inflammatory activities.

Keywords

References

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