Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Anti-inflammatory Effects of New Herbal Formula Cheongyeoungkeumyeum

청영금음(淸營金飮)이 자유산소라디칼과 염증매개인자에 미치는 영향

  • 김승태 (부산대학교 한의학전문대학원) ;
  • 이부균 (부산대학교 한의학전문대학원) ;
  • 우원홍 (원광대학교 한의과대학 해부학교실) ;
  • 김형우 (부산대학교 한의학전문대학원) ;
  • 이영철 (상지대학교 한의과대학 본초학교실) ;
  • 이정복 (소니메디) ;
  • 이장천 (부산대학교 한의학전문대학원)
  • Received : 2012.03.16
  • Accepted : 2012.04.18
  • Published : 2012.04.25
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Abstract

The purpose of this study is to verify the anti-inflammatory effectiveness of Cheongyeoungkeumyeum(CYK) including seven herbal medicines of that major effectiveness is to clear heat, to relieve fire toxicity and to clear damp-heat. To evaluate anti-inflammatory effectiveness of CYK, we measured the production of reactive oxygen species(ROS), nitric oxide(NO) cyclooxygenase-2(COX-2) and in TNF-${\alpha}$ LPS-activated Raw 264.7 cells. Cell viability was determined by MTT assay. The concentrations of ROS and relative level of NO were measured with DPPH assay and Griess reagent, respectively. COX-2 and TNF-${\alpha}$ were detected by enzyme immuno assay(EIA) and enzyme-linked immunosorbent assay(ELISA). As a result, we found that CYK suppressed LPS-induced ROS and NO production in a dose-dependent manner. Also CYK significantly inhibited LPS-induced COX-2 activity and the release of TNF-${\alpha}$. These results indicate that the CYK may have an anti-inflammatory agent for the treatment of various inflammatory disease.

Keywords

References

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