Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Extract of Alnus japonica Induces Apoptosis of Human Colon Adenocarcinoma Cells through the Mitochondria/Caspase Pathway

대장암세포주에서 적양 추출물의 미토콘드리아/Caspases 경로를 통한 Apoptosis 유도 작용

  • Jeon, Byoung-Kook (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Baik, Soon-Ki (Department of Environmental Engineering, Dongshin University) ;
  • Woo, Won-Hong (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Mun, Yeun-Ja (Department of of Anatomy, College of Oriental Medicine, Wonkwang University)
  • 전병국 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 백순기 (동신대학교 공과대학 환경학과) ;
  • 우원홍 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 문연자 (원광대학교 한의과대학 해부학교실)
  • Received : 2012.02.21
  • Accepted : 2012.04.18
  • Published : 2012.04.25
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Abstract

An extract of Alnus japonica (Betulaceae) cortex has been traditionally used for purifying blood, and curing feces containing blood, enteritis, diarrhea, alcoholism and cut wounds. In the present study, we demonstrated that the ethanol extract of Alnus japonica (EAJ) exhibited significantly cytotoxicity in human colon adenocarcinoma HT-29 cells. The results showed that the induction of apoptosis in HT-29 cells by EAJ was characterized by chromatin condensation and activation of caspase-3. EAJ-induced activation of caspase-9 and -3 caused the cleavage of poly ADP-ribose polymerase (PARP) and the release of cytochrome c. The expressions of Bcl-2 and Bid were reduced by EAJ in HT-29 cells, whereas pro-apoptotic protein Bak was increased in the cells. EAJ-induced, dose-dependent induction of apoptosis was accompanied by sustained phosphorylation of MAP kinases (JNK and p38 MAPK), ASK1, and p53. NAC administration, a scavenger of ROS, reversed EAJ-induced cell death. In conclusion, these results indicated that EAJ can cause apoptosis through a ROS-mitochondria-caspases-dependent pathway in human HT-29 cells.

Keywords

References

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