Asian Pacific Journal of Cancer Prevention

  • 제13권5호
  • /
  • Pages.1793-1797
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  • 2012
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  • 1513-7368(pISSN)
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  • 2476-762X(eISSN)
아시아태평양암예방학회 (Asian Pacific Journal of Cancer Prevention)
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Neurotrophic Artemin Promotes Motility and Invasiveness of MIA PaCa-2 Pancreatic Cancer Cells

  • Meng, Ling-Xin (Department of Oncology, Affiliated Rizhao People's Hospital, Jining Medical University) ;
  • Chi, Yu-Hua (Department of Oncology, Affiliated Rizhao People's Hospital, Jining Medical University) ;
  • Wang, Xiang-Xu (Department of Oncology, Affiliated Rizhao People's Hospital, Jining Medical University) ;
  • Ding, Zhao-Jun (Department of Oncology, Affiliated Rizhao People's Hospital, Jining Medical University) ;
  • Fei, Li-Cong (Department of Oncology, Affiliated Rizhao People's Hospital, Jining Medical University) ;
  • Zhang, Hong (Department of Oncology, Affiliated Rizhao People's Hospital, Jining Medical University) ;
  • Mou, Ling (Department of Oncology, Affiliated Rizhao People's Hospital, Jining Medical University) ;
  • Cui, Wen (Department of Oncology, Affiliated Rizhao People's Hospital, Jining Medical University) ;
  • Xue, Ying-Jie (Department of Oncology, Affiliated Rizhao People's Hospital, Jining Medical University)
  • 발행 : 2012.05.30
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초록

Objective: To analyze the capacity of neurotrophic artemin to promote the motility and invasiveness of MIA PaCa-2 pancreatic cancer cells. Methods: MIA PaCa-2 was cultured in vitro and studied using transwell chambers for motility and invasiveness on treatment with different concentrations of aArtemin or its receptor $GFR{\alpha}3$ were also determined. Expression of matrix metalloproteinase-2 (MMP-2) and epithelial cadherin (E-cadherin) was quantified using RT-PCR and Western blotting. Results: MIA PaCa-2 pancreatic cancer cell motility and invasiveness was significantly increased with artemin and its receptor $GFR{\alpha}3$ with dose dependence (P<0.01). MMP-2 production was also significantly increased (t = 6.35, t = 7.32), while E-cadherin was significantly lowered (t = 4.27, t = 5.61) (P <0.01). Conclusion: Artemin and its receptor $GFR{\alpha}3$ can promote pancreatic cancer cell motility and invasiveness and contribute to aggressive behavior. The mechanism may be related to increased expression of MMP-2 molecule and down-regulation of E-cadherin expression.

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참고문헌

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