DOI QR코드

DOI QR Code

siRNA Mediated Silencing of NIN1/RPN12 Binding Protein 1 Homolog Inhibits Proliferation and Growth of Breast Cancer Cells

  • Huang, Wei-Yi (Department of Oncology, Affiliated People's 1st Hospital, Shanghai Jiaotong University) ;
  • Chen, Dong-Hui (Department of Oncology, Affiliated People's 1st Hospital, Shanghai Jiaotong University) ;
  • Ning, Li (Department of Oncology, Affiliated People's 1st Hospital, Shanghai Jiaotong University) ;
  • Wang, Li-Wei (Department of Oncology, Affiliated People's 1st Hospital, Shanghai Jiaotong University)
  • 발행 : 2012.05.30

초록

The gene encoding the Nin one binding (NOB1) protein which plays an essential role in protein degradation has been investigated for possible tumor promoting functions. The present study was focused on NOB1 as a possible therapeutic target for breast cancer treatment. Lentivirus mediated NOB1 siRNA transfection was used to silence the NOB1 gene in two established breast cancer cell lines, MCF-7 and MDA-MB-231, successful transfection being confirmed by fluorescence imaging. NOB1 deletion caused significant decline in cell proliferation was observed in both cell lines as investigated by MTT assay. Furthermore the number and size of the colonies formed were also significantly reduced in the absence of NOB1. Moreover NOB1 gene knockdown arrested the cell cycle and inhibited cell cycle related protein expression. Collectively these results indicate that NOB1 plays an essential role in breast cancer cell proliferation and its gene expression could be a therapeutic target.

키워드

참고문헌

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피인용 문헌

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  4. Downregulation of NOB1 suppresses the proliferation and tumor growth of non-small cell lung cancer in vitro and in vivo vol.31, pp.3, 2014, https://doi.org/10.3892/or.2014.2991
  5. Adenovirus-mediated siRNA targeting NOB1 inhibits tumor growth and enhances radiosensitivity of human papillary thyroid carcinoma in vitro and in vivo vol.32, pp.6, 2014, https://doi.org/10.3892/or.2014.3483
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  9. Lentivirus-mediated gene silencing of NOB1 suppresses non-small cell lung cancer cell proliferation vol.34, pp.3, 2015, https://doi.org/10.3892/or.2015.4132
  10. Downregulation of NOB1 inhibits proliferation and promotes apoptosis in human oral squamous cell carcinoma vol.34, pp.6, 2015, https://doi.org/10.3892/or.2015.4271
  11. Silencing NOB1 enhances doxorubicin antitumor activity of the papillary thyroid carcinoma in vitro and in vivo pp.1791-2431, 2015, https://doi.org/10.3892/or.2015.3730
  12. NOB1 silencing inhibits the growth and metastasis of laryngeal cancer cells through the regulation of JNK signaling pathway vol.35, pp.6, 2016, https://doi.org/10.3892/or.2016.4707
  13. miR-192 suppresses the tumorigenicity of prostate cancer cells by targeting and inhibiting nin one binding protein vol.37, pp.2, 2016, https://doi.org/10.3892/ijmm.2016.2449