Asian Pacific Journal of Cancer Prevention

  • 제13권5호
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  • Pages.2299-2304
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  • 2012
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  • 1513-7368(pISSN)
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  • 2476-762X(eISSN)
아시아태평양암예방학회 (Asian Pacific Journal of Cancer Prevention)
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Detection of Human Papillomavirus in Normal Oral Cavity in a Group of Pakistani Subjects using Real-Time PCR

  • 발행 : 2012.05.30
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초록

Since there is evidence that human papillomavirus (HPV) may play some role in oral carcinogenesis, we investigated the presence of HPV in a group of Pakistani subjects with normal oral cavity using real-time PCR analysis. Two-hundred patients attending the Dental Department, Sandaman Provincial Hospital, Balochistan, Pakistan, were recruited. After interview, oral epithelial cells were collected by scraping and subjected to DNA extraction. The HPV-positive DNA samples were further analyzed using primer sets specific for HPV-16 and -18. It was found that out of 200 DNA samples, 192 were PCR-positive for the ${\beta}$-globin gene and these were subsequently examined for the presence of HPV DNA. Among these, 47 (24.5%) were HPV-positive with the virus copy number ranged between 0.43-32 copies per 1 ${\mu}g$ of total DNA (9-99 copies per PCR reaction). There were 4 and 11 samples containing HPV-16 and -18, respectively. Additionally, one sample harbored both types of HPV. Among the investigated clinical parameters, smoking habit was associated with the presence of HPV (p = 0.001) while others indicated no significant association. The prevalence of HPV in normal oral cavity in our Pakistani subjects appears to be comparable to other studies. However, the association between the presence of HPV and smoking warrants further investigations whether both of these factors can cooperate in inducing oral cancer in this group of patients.

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  3. Detection of Salivary Human Papilloma Viruses 16 and 18 (HPV) in Smoker Men in an Iranian Population by PCR: A Pilot Study vol.3, pp.3, 2014, https://doi.org/10.5812/ijhrba.18053
  4. Do Human Papilloma Viruses Play Any Role in Oral Squamous Cell Carcinoma in North Indians? vol.16, pp.16, 2015, https://doi.org/10.7314/APJCP.2015.16.16.7077
  5. Knowledge of Human Papillomavirus and its Association with Head and Neck Benign and Malignant Lesions in a Group of Dental Patients in Pakistan vol.16, pp.4, 2015, https://doi.org/10.7314/APJCP.2015.16.4.1581
  6. Molecular diagnosis and phylogenetic analysis of human papillomavirus type-16 from suspected patients in Pakistan vol.11, pp.1, 2016, https://doi.org/10.1186/s13027-016-0047-z