The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Spontaneous Electrical Activity of Cultured Interstitial Cells of Cajal from Mouse Urinary Bladder

  • Kim, Sun-Ouck (Department of Urology, Chonnam National University Medical School) ;
  • Jeong, Han-Seong (Department of Physiology, Chonnam National University Medical School) ;
  • Jang, Sujeong (Department of Physiology, Chonnam National University Medical School) ;
  • Wu, Mei-Jin (Department of Physiology, Chonnam National University Medical School) ;
  • Park, Jong Kyu (Department of Neurosurgery, College of Medicine, Chosun University) ;
  • Jiao, Han-Yi (Department of Physiology, College of Medicine, Chosun University) ;
  • Jun, Jae Yeoul (Department of Physiology, College of Medicine, Chosun University) ;
  • Park, Jong-Seong (Department of Physiology, Chonnam National University Medical School)
  • Received : 2013.11.06
  • Accepted : 2013.12.03
  • Published : 2013.12.30
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Abstract

Interstitial cells of Cajal (ICCs) from the urinary bladder regulate detrusor smooth muscle activities. We cultured ICCs from the urinary bladder of mice and performed patch clamp and intracellular $Ca^{2+}$ ($[Ca^{2+}]_i$) imaging to investigate whether cultured ICCs can be a valuable tool for cellular functional studies. The cultured ICCs displayed two types of spontaneous electrical activities which are similar to those recorded in intact bladder tissues. Spontaneous electrical activities of cultured ICCs were nifedipine-sensitive. Carbachol and ATP, both excitatory neurotransmitters in the urinary bladder, depolarized the membrane and increased the frequency of spike potentials. Carbachol increased $[Ca^{2+}]_i$ oscillations and basal $Ca^{2+}$ levels, which were blocked by atropine. These results suggest that cultured ICCs from the urinary bladder retain rhythmic phenotypes similar to the spontaneous electrical activities recorded from the intact urinary bladder. Therefore, we suggest that cultured ICCs from the urinary bladder may be useful for cellular and molecular studies of ICCs.

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References

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