The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Potassium Currents in Isolated Deiters' Cells of Guinea Pig

  • Chung, Jong Woo (Department of Otolaryngology, Asan Medical Center and University of Ulsan College of Medicine) ;
  • Nam, Eui Chol (Department of Otolaryngology, Kangwon National University College of Medicine) ;
  • Kim, Won Tae (Department of Physiology, Asan Medical Center and University of Ulsan College of Medicine) ;
  • Youm, Jae Boum (Department of Physiology, College of Medicine, Cardiovascular and Metabolic Disease Center, Inje University) ;
  • Leem, Chae Hun (Department of Physiology, Asan Medical Center and University of Ulsan College of Medicine)
  • Received : 2013.11.09
  • Accepted : 2013.12.02
  • Published : 2013.12.30
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Abstract

Deiters' cells are the supporting cells in organ of Corti and are suggested to play an important role in biochemical and mechanical modulation of outer hair cells. We successfully isolated functionally different $K^+$ currents from Deiters' cells of guinea pig using whole cell patch clamp technique. With high $K^+$ pipette solution, depolarizing step pulses activated strongly outward rectifying currents which were dose-dependently blocked by clofilium, a class III anti-arrhythmic $K^+$ channel blocker. The remaining outward current was transient in time course whereas the clofilium-sensitive outward current showed slow inactivation and delayed rectification. Addition of 5 mM tetraethylammonium (TEA) further blocked the remaining current leaving a very fast inactivating transient outward current. Therefore, at least three different types of $K^+$ current were identified in Deiters' cells, such as fast activating and fast inactivating current, fast activating slow inactivating current, and very fast inactivating transient outward current. Physiological role of them needs to be established.

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References

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