Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effects of Serial Passage on the Characteristics and Chondrogenic Differentiation of Canine Umbilical Cord Matrix Derived Mesenchymal Stem Cells

  • Lee, K.S. (Viral Disease Division, Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Cha, S.H. (Research Planning Division, Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Kang, H.W. (Viral Disease Division, Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Song, J.Y. (Viral Disease Division, Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Lee, K.W. (Viral Disease Division, Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Ko, K.B. (Research Planning Division, Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Lee, H.T. (Department of Animal Biotechnology, Konkuk University)
  • Received : 2012.09.10
  • Accepted : 2012.11.13
  • Published : 2013.04.01
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Abstract

Mesenchymal stem cells (MSCs) are often known to have a therapeutic potential in the cell-mediated repair for fatal or incurable diseases. In this study, canine umbilical cord MSCs (cUC-MSCs) were isolated from umbilical cord matrix (n = 3) and subjected to proliferative culture for 5 consecutive passages. The cells at each passage were characterized for multipotent MSC properties such as proliferation kinetics, expression patterns of MSC surface markers and self-renewal associated markers, and chondrogenic differentiation. In results, the proliferation of the cells as determined by the cumulative population doubling level was observed at its peak on passage 3 and stopped after passage 5, whereas cell doubling time dramatically increased after passage 4. Expression of MSC surface markers (CD44, CD54, CD61, CD80, CD90 and Flk-1), molecule (HMGA2) and pluripotent markers (sox2, nanog) associated with self-renewal was negatively correlated with the number of passages. However, MSC surface marker (CD105) and pluripotent marker (Oct3/4) decreased with increasing the number of subpassage. cUC-MSCs at passage 1 to 5 underwent chondrogenesis under specific culture conditions, but percentage of chondrogenic differentiation decreased with increasing the number of subpassage. Collectively, the present study suggested that sequential subpassage could affect multipotent properties of cUC-MSCs and needs to be addressed before clinical applications.

Keywords

References

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