Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
권호 표지
DOI QR코드

DOI QR Code

Selective Effects of Curcumin on CdSe/ZnS Quantum-dot-induced Phototoxicity Using UVA Irradiation in Normal Human Lymphocytes and Leukemia Cells

  • Goo, Soomin (School of Public Health and Institute of Health and Environment, Seoul National University) ;
  • Choi, Young Joo (School of Public Health and Institute of Health and Environment, Seoul National University) ;
  • Lee, Younghyun (School of Public Health and Institute of Health and Environment, Seoul National University) ;
  • Lee, Sunyeong (School of Public Health and Institute of Health and Environment, Seoul National University) ;
  • Chung, Hai Won (School of Public Health and Institute of Health and Environment, Seoul National University)
  • Received : 2013.01.30
  • Accepted : 2013.03.18
  • Published : 2013.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

Quantum dots (QDs) have received considerable attention due to their potential role in photosensitization during photodynamic therapy. Although QDS are attractive nanomaterials due to their novel and unique physicochemical properties, concerns about their toxicity remain. We suggest a combination strategy, CdSe/ZnS QDs together with curcumin, a natural yellow pigment from turmeric, to reduce QD-induced cytotoxicity. The aim of this study was to explore a potentially effective cancer treatment: co-exposure of HL-60 cells and human normal lymphocytes to CdSe/ZnS QDs and curcumin. Cell viability, apoptosis, reactive oxygen species (ROS) generation, and DNA damage induced by QDs and/or curcumin with or without ultraviolet A (UVA) irradiation were evaluated in both HL-60 cells and normal lymphocytes. In HL-60 cells, cell death, apoptosis, ROS generation, and single/double DNA strand breaks induced by QDs were enhanced by treatment with curcumin and UVA irradiation. The protective effects of curcumin on cell viability, apoptosis, and ROS generation were observed in normal lymphocytes, but not leukemia cells. These results demonstrated that treatment with QD combined with curcumin increased cell death in HL-60 cells, which was mediated by ROS generation. However, curcumin acted as an antioxidant in cultured human normal lymphocytes.

Keywords

References

  1. Hauck, T.S., Anderson, R.E., Fischer, H.C., Newbigging, S. and Chan, W.C. (2010) In vivo quantum-dot toxicity assessment. Small, 6, 138-144. https://doi.org/10.1002/smll.200900626
  2. Choi, Y.J., Kim, Y.J., Lee, J.W., Lee, Y., Lim, Y.B. and Chung, H.W. (2012) Cyto-/genotoxic effect of CdSe/ZnS quantum dots in human lung adenocarcinoma cells for potential photodynamic UV therapy applications. J. Nanosci. Nanotechnol., 12, 2160-2168. https://doi.org/10.1166/jnn.2012.5781
  3. Chibli, H., Carlini, L., Park, S., Dimitrijevic, N.M. and Nadeau, J.L. (2011) Cytotoxicity of InP/ZnS quantum dots related to reactive oxygen species generation. Nanoscale, 3, 2552-2559. https://doi.org/10.1039/c1nr10131e
  4. Morosini, V., Bastogne, T., Frochot, C., Schneider, R., François, A., Guillemin, F. and Barberi-Heyob, M. (2011) Quantum dot-folic acid conjugates as potential photosensitizers in photodynamic therapy of cancer. Photochem. Photobiol. Sci., 10, 842-851. https://doi.org/10.1039/c0pp00380h
  5. Pons, T., Pic, E., Lequeux, N., Cassette, E., Bezdetnaya, L., Guillemin, F., Marchal, F. and Dubertret, B. (2010) Cadmiumfree CuInS2/ZnS quantum dots for sentinel lymph node imaging with reduced toxicity. ACS Nano, 4, 2531-2538. https://doi.org/10.1021/nn901421v
  6. Valizaden, A., Mikaeili, H., Samiel, M., Farkhani, S.M., Zarghami, N., Kouhi, M., Akbarzadeh, A. and Davaran, S. (2012) Quantum dots: synthesis, bioapplications, and toxicity. Nanoscale Res. Lett., 7, 480. https://doi.org/10.1186/1556-276X-7-480
  7. Byrne, S.J., Williams, Y., Davies, A., Corr, S.A., Rakovich, A., Gun'ko, Y.K., Rakovich, Y.P., Donegan, J.F. and Volkov, Y. (2007) "Jelly dots": synthesis and cytotoxicity studies of CdTe quantum dot-gelatin nanocomposites. Small, 3, 1152-1156. https://doi.org/10.1002/smll.200700090
  8. Mazumder, S., Dey, R., Mitra, M.K., Mukherjee, S. and Das, G.C. (2009) Review: Biofunctionalized quantum dots in biol ogy and medicine. J. Nanomater., Article ID 815734, 17.
  9. Dovigo, L.N., Pavarina, A.C., Ribeiro, A.P., Brunetti, I.L., Costa, C.A., Jacomassi, D.P., Bagnato, V.S. and Kurachi, C. (2011) Investigation of the photodynamic effects of curcumin against Candida albicans. Photochem. Photobiol., 87, 895- 903. https://doi.org/10.1111/j.1751-1097.2011.00937.x
  10. Zhang, B.Y., Shi, Y.Q., Chen, X., Dai, J., Jiang, Z.F., Li, N. and Zhang, Z.B. (2012) Protective effect of curcumin against formaldehyde-induced genotoxicity in A549 cell lines. J. Appl. Toxicol., DOI 10.1002/jat.2814.
  11. Ayli, E.E., Dugas-Breit, S., Li, W., Marshall, C., Zhao, L., Meulener, M., Griffin, T., Gelfand, J.M. and Seykora, J.T. (2010) Curcuminoids activate p38 MAP kinases and promote UVB-dependent signaling in keratinocytes. Exp. Dermatol., 19, 493-500. https://doi.org/10.1111/j.1600-0625.2010.01081.x
  12. Huang, H.C., Lin, C.J., Liu, W.J., Jiang, R.R. and Jiang, Z.F. (2011) Dual effects of curcumin on neuronal oxidative stress in the presence of Cu(II). Food Chem. Toxicol., 49, 1578-1583. https://doi.org/10.1016/j.fct.2011.04.004
  13. Wilken, R., Veena, M.S., Wang, M.B. and Srivatsan, E.S. (2011) Curcumin: A review of anti-cancer properties and therapeutic activity in head and neck squamous cell carcinoma. Mol. Cancer, 10, 10-12. https://doi.org/10.1186/1476-4598-10-10
  14. Ahn, J.C., Kang, J.W., Shin, J.I. and Chung, P.S. (2012) Combination treatment with photodynamic therapy and curcumin induces mitochondria-dependent apoptosis in AMC-HN3 cells. Int. J. Oncol., 41, 2184-2190.
  15. Park, K. and Lee, J.H. (2007) Photosensitizer effect of curcumin on UVB-irradiated HaCaT cells through activation of caspase pathways. Oncol. Rep., 17, 537-540.
  16. Singh, N.P., McCoy, M.T., Tice, R.R. and Schneider, E.L. (1988) A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell. Res., 175, 184-191. https://doi.org/10.1016/0014-4827(88)90265-0
  17. Fenech, M. (1993) The cytokinesis-block micronucleus technique: a detailed description of the method and its application to genotoxicity studies in human populations. Mutat. Res., 285, 35-44. https://doi.org/10.1016/0027-5107(93)90049-L
  18. Fenech, M. (2000) The in vitro micronucleus technique. Mutat. Res., 455, 81-95. https://doi.org/10.1016/S0027-5107(00)00065-8
  19. Clift, M.J. and Stone, V. (2012) Quantum dots: an insight and perspective of their biological interaction and how this relates to their relevance for clinical use. Theranostics, 2, 668-680. https://doi.org/10.7150/thno.4545
  20. Ahsan, H. and Hadi, S.M. (1998) Strand scission in DNA induced by curcumin in the presence of Cu(II). Cancer Lett., 124, 23-30. https://doi.org/10.1016/S0304-3835(97)00442-4
  21. Mahakunakorn, P., Tohda, M., Murakami, Y., Matsumoto, K., Watanabe, H. and Vajaragupta, O. (2003) Cytoprotective and cytotoxic effects of curcumin: dual action on H2O2-induced oxidative cell damage in NG108-15 cells. Biol. Pharm. Bull., 26, 725-728. https://doi.org/10.1248/bpb.26.725
  22. Cao, J., Jia, L., Zhou, H.M., Liu, Y. and Zhong, L.F. (2006) Mitochondrial and nuclear DNA damage induced by curcumin in human hepatoma G2 cells. Toxicol. Sci. 91, 476-483. https://doi.org/10.1093/toxsci/kfj153
  23. Choudhuri, T., Pal, S., Das, T. and Sa, G. (2005) Curcumin selectively induces apoptosis in deregulated cyclin D1- expressed cells at G2 phase of cell cycle in a p53-dependent manner. J. Biol. Chem., 280, 20059-20068. https://doi.org/10.1074/jbc.M410670200
  24. Syng-Ai, C., Kumari, A.L. and Khar, A. (2004) Effect of curcumin on normal and tumor cells: Role of glutathione and bcl- 2. Mol. Cancer Ther., 3, 1101-1108.
  25. Kunwar, A., Barik, A., Mishra, B., Rathinasamy, K., Pandey, R. and Priyadarsini, K.I. (2008) Quantitative cellular uptake, localization and cytotoxicity of curcumin in normal and tumor cells. Biochim. Biophys. Acta, 1780, 673-679. https://doi.org/10.1016/j.bbagen.2007.11.016
  26. Ravindran, J., Prasad, S. and Aggarwal, B.B. (2009) Curcumin and cancer cells: how many ways can curry kill tumor cells selectively? AAPS J., 11, 495-510. https://doi.org/10.1208/s12248-009-9128-x

Cited by

  1. Meta-analysis of cellular toxicity for cadmium-containing quantum dots vol.11, pp.5, 2016, https://doi.org/10.1038/nnano.2015.338
  2. Nrf2-Mediated HO-1 Induction Contributes to Antioxidant Capacity of a Schisandrae Fructus Ethanol Extract in C2C12 Myoblasts vol.6, pp.12, 2014, https://doi.org/10.3390/nu6125667
  3. Curcumin in chronic lymphocytic leukemia – A review vol.7, pp.6, 2017, https://doi.org/10.1016/j.apjtb.2017.05.003