The Korean Journal of Physiology and Pharmacology

  • 제17권2호
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  • Pages.163-167
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  • 2013
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  • 1226-4512(pISSN)
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  • 2093-3827(eISSN)
대한약리학회 (The Korean Society of Pharmacology)
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Effect of Cholera Toxin Administered Supraspinally or Spinally on the Blood Glucose Level in Pain and D-Glucose Fed Animal Models

  • Sim, Yun-Beom (Department of Pharmacology, Institute of Natural Medicine, College of Medicine, Hallym University) ;
  • Park, Soo-Hyun (Department of Pharmacology, Institute of Natural Medicine, College of Medicine, Hallym University) ;
  • Kang, Yu-Jung (Department of Pharmacology, Institute of Natural Medicine, College of Medicine, Hallym University) ;
  • Kim, Sung-Su (Department of Pharmacology, Institute of Natural Medicine, College of Medicine, Hallym University) ;
  • Kim, Chea-Ha (Department of Pharmacology, Institute of Natural Medicine, College of Medicine, Hallym University) ;
  • Kim, Su-Jin (Department of Pharmacology, Institute of Natural Medicine, College of Medicine, Hallym University) ;
  • Jung, Jun-Sub (Department of Pharmacology, Institute of Natural Medicine, College of Medicine, Hallym University) ;
  • Ryu, Ohk-Hyun (Division of Endocrinology and Metabolism, Department of Internal Medicine, College of Medicine, Hallym University) ;
  • Choi, Moon-Gi (Division of Endocrinology and Metabolism, Department of Internal Medicine, College of Medicine, Hallym University) ;
  • Choi, Seong-Soo (Department of Anesthesiology and Pain Medicine, Asan Medical Center and University of Ulsan College of Medicine) ;
  • Suh, Hong-Won (Department of Pharmacology, Institute of Natural Medicine, College of Medicine, Hallym University)
  • 투고 : 2012.08.09
  • 심사 : 2013.01.21
  • 발행 : 2013.04.30
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초록

In the present study, the effect of intrathecal (i.t.) or intracerebroventricular (i.c.v.) administration with cholera toxin (CTX) on the blood glucose level was examined in ICR mice. The i.t. treatment with CTX alone for 24 h dose-dependently increased the blood glucose level. However, i.c.v. treatment with CTX for 24 h did not affect the blood glucose level. When mice were orally fed with D-glucose (2 g/kg), the blood glucose level reached to a maximum level at 30 min and almost returned to the control level at 120 min after D-glucose feeding. I.c.v. pretreatment with CTX increased the blood glucose level in a potentiative manner, whereas i.t. pretreatment with CTX increased the blood glucose level in an additive manner in a D-glucose fed group. In addition, the blood glucose level was increased in formalin-induced pain animal model. I.c.v. pretreatment with CTX enhanced the blood glucose level in a potentiative manner in formalin-induced pain animal model. On the other hand, i.t. pretreatment with CTX increased the blood glucose level in an additive manner in formalin-induced pain animal model. Our results suggest that CTX administered supraspinally or spinally differentially modulates the regulation of the blood glucose level in D-glucose fed model as well as in formalin-induced pain model.

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참고문헌

  1. de los Santos-Arteaga M, Sierra-Dominguez SA, Fontanella GH, Delgado-Garcia JM, Carrion AM. Analgesia induced by dietary restriction is mediated by the kappa-opioid system. J Neurosci. 2003;23:11120-11126.
  2. Blackburn-Munro G, Blackburn-Munro R. Pain in the brain: are hormones to blame? Trends Endocrinol Metab. 2003;14:20-27. https://doi.org/10.1016/S1043-2760(02)00004-8
  3. Won JS, Suh HW. The comparative analysis of proenkephalin mRNA expression induced by cholera toxin and pertussis toxin in primary cultured rat cortical astrocytes. Brain Res Mol Brain Res. 2001;88:83-93. https://doi.org/10.1016/S0169-328X(01)00031-6
  4. Bullock BP, Habener JF. Phosphorylation of the cAMP response element binding protein CREB by cAMP-dependent protein kinase A and glycogen synthase kinase-3 alters DNA-binding affinity, conformation, and increases net charge. Biochemistry. 1998;37:3795-3809. https://doi.org/10.1021/bi970982t
  5. Kaupp UB. The cyclic nucleotide-gated channels of vertebrate photoreceptors and olfactory epithelium. Trends Neurosci. 1991;14:150-157. https://doi.org/10.1016/0166-2236(91)90087-B
  6. Facci L, Skaper SD, Favaron M, Leon A. A role for gangliosides in astroglial cell differentiation in vitro. J Cell Biol. 1988;106: 821-828. https://doi.org/10.1083/jcb.106.3.821
  7. Chung KM, Lee KC, Song DK, Huh SO, Choi MR, Kim YH, Suh HW. Differential modulatory roles of cholera toxin and pertussis toxin in the regulation of pain responses induced by excitatory amino acids administered intrathecally in mice. Brain Res. 2000;867:246-249. https://doi.org/10.1016/S0006-8993(00)02287-3
  8. Suh HW, Song DK, Sim YB, Choi YS, Kim YH. Effect of intrathecal or intracerebroventricular pretreatment with cholera toxin on antinociception induced by opioids administered intracerebroventricularly in mice. Pharmacol Commun. 1995;6: 187-294.
  9. Sim YB, Park SH, Kang YJ, Jung JS, Ryu OH, Choi MG, Suh HW. Various pain stimulations cause an increase of the blood glucose level. Animal Cells and Systems. 2012;16:385-390. https://doi.org/10.1080/19768354.2012.696554
  10. Kwon MS, Shim EJ, Seo YJ, Choi SS, Lee JY, Lee HK, Suh HW. Differential modulatory effects of cholera toxin and pertussis toxin on pain behavior induced by TNF-alpha, interleukin- 1beta and interferon-gamma injected intrathecally. Arch Pharm Res. 2005;28:582-586. https://doi.org/10.1007/BF02977762
  11. Hylden JL, Wilcox GL. Intrathecal substance P elicits a caudally- directed biting and scratching behavior in mice. Brain Res. 1981;217:212-215. https://doi.org/10.1016/0006-8993(81)90203-1
  12. Haley TJ. Pharmacological effects produced by intracerebral administration of drugs of unrelated structure to conscious mice. Arch Int Pharmacodyn Ther. 1957;110:239-244.
  13. Hunskaar S, Berge OG, Hole K. Antinociceptive effects of orphenadrine citrate in mice. Eur J Pharmacol. 1985;111:221-226. https://doi.org/10.1016/0014-2999(85)90759-9
  14. Sim YB, Park SH, Kang YJ, Jung JS, Ryu OH, Choi MG, Suh HW. Interleukin-1$\beta$ (IL-1$\beta$) increases pain behavior and the blood glucose level: possible involvement of sympathetic nervous system. Pharmacol Biochem Behav. 2012;102:170-176. https://doi.org/10.1016/j.pbb.2012.04.006
  15. Sim YB, Park SH, Kang YJ, Kim SS, Kim CH, Kim SJ, Jung JS, Ryu OH, Choi MG, Suh HW. Central anti-diabetic action of biguanide and thizolidinediones in D-glucose fed and streptozotocin- treated mouse models. Neurosci Lett. 2012;528:73-77. https://doi.org/10.1016/j.neulet.2012.08.038
  16. Sala F, Menna G, Bricolo A, Young W. Role of glycemia in acute spinal cord injury. Data from a rat experimental model and clinical experience. Ann N Y Acad Sci. 1999;890:133-154. https://doi.org/10.1111/j.1749-6632.1999.tb07989.x