Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
권호 표지
DOI QR코드

DOI QR Code

Comparison between Source-induced Dissociation and Collision-induced Dissociation of Ampicillin, Chloramphenicol, Ciprofloxacin, and Oxytetracycline via Mass Spectrometry

  • Lee, Seung Ha (Department of Environmental Health, College of Health Sciences, Korea University) ;
  • Choi, Dal Woong (Department of Environmental Health, College of Health Sciences, Korea University)
  • Received : 2013.06.21
  • Accepted : 2013.06.25
  • Published : 2013.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Mass spectrometry (MS) is a very powerful instrument that can be used to analyze a wide range of materials such as proteins, peptides, DNA, drugs, and polymers. The process typically involves either chemical or electron (impact) ionization of the analyte. The resulting charged species or fragment is subsequently identified by the detector. Usually, single mass uses source-induced dissociation (SID), whereas mass/mass uses collision-induced dissociation (CID) to analyze the chemical fragmentations Each technique has its own advantages and disadvantages. While CID is most effective for the analysis of pure substances, multiple-step MS is a powerful technique to get structural data. Analysis of veterinary drugs ampicillin, chloramphenicol, ciprofloxacin, and oxytetracycline serves to highlight the slight differences between SID and CID. For example, minor differences were observed between ciprofloxacin and oxytetracycline via SID or CID. However, distinct fragmentation patterns were observed for ampicllin depending on the analysis method. Both SID and CID showed similar fragmentation spectra but different signal intensities for chloramphenicol. There are several factors that can influence the fragmentation spectra, such as the collision energy, major precursor ion, electrospray mode (positive or negative), and sample homogeneity. Therefore, one must select a fragmentation method on an empirical and case-by-case basis.

Keywords

References

  1. Gabelica, V. and De Pauw, E. (2005) Internal energy and fragmentation of ions produced in electrospray sources. Mass Spectrom. Rev., 24, 566-587. https://doi.org/10.1002/mas.20027
  2. Abranko, L., Garcia-Reyes, J.F. and Molina-Diaz, A. (2011) In-source fragmentation and accurate mass analysis of multiclass flavonoid conjugates by electrospray ionization time-offlight mass spectrometry. J. Mass Spectrom., 46, 478-488. https://doi.org/10.1002/jms.1914
  3. Tiller, P.R. and Drexler, D.M. (1999) Evaluating the Differences between Source CID or Real MS/MS, Thermo Finnigan LC/MS Technical Report, A0895-776, 719.
  4. Marquet, P., Venisse, N., Lacassie, E. and Lachatre, G. (2000) In-source CID mass spectral libraries for the "general unknown" screening of drugs and toxicants. Analusis, 28, 925-934. https://doi.org/10.1051/analusis:2000280925
  5. Abdelhameed, A.S., Attwa, M.W., Abdel-Aziz, H.A. and Kadi, A.A. (2013) Induced in-source fragmentation pattern of certain novel (1Z,2E)-N-(aryl)propanehydrazonoyl chlorides by electrospray mass spectrometry (ESl-MS/MS). Chem. Cent. J., 7, 16. https://doi.org/10.1186/1752-153X-7-16
  6. Korfmacher, W.A. (2005) Principles and applications of LCMS in new drug discovery. Drug Discovery Today, 10, 1357-1367. https://doi.org/10.1016/S1359-6446(05)03620-2
  7. Okerman, L., Croubels, S., De Baere, S., Van Hoof, J., De Backer, P. and De Brabander, H. (2001) Inhibition tests for detection and presumptive identification of tetracyclines, betalactam antibiotics and quinolones in poultry meat. Food Addit. Contam., 18, 385-393. https://doi.org/10.1080/02652030120410
  8. Wang, J. (2004) Confirmatory determination of six penicillins in honey by liquid chromatography/electrospray ionizationtandem mass spectrometry. J. AOAC Int., 87, 45-55.
  9. Ghidini, S., Zanardi, E., Varisco, G. and Chizzolini, R. (2002) Prevalence of molecules of $\beta$-lactam antibiotics in bovine milk in lombardia and emilia romagna (Italy). Ann. Fac. Medic. Vet. di Parma, 22, 245-252.
  10. Gantverg, A., Shishani, I. and Hoffman, M. (2003) Determination of chloramphenicol in animal tissues and urine: Liquid chromatography-tandem mass spectrometry versus gas chromatography- mass spectrometry. Anal. Chim. Acta, 483, 125-135. https://doi.org/10.1016/S0003-2670(02)01566-0
  11. Rupp, H.S., Stuart, J.S. and Hurlbut, J.A. (2005) Liquid chromatography/ tandem mass spectrometry analysis of chloramphenicol in cooked crab meat. J. AOAC Int., 88, 1155-1159.
  12. Pfenning, A., Turnipseed, S., Roybal, J., Madson, M., Lee, R. and Storey, J. (2003) Confirmation of chloramphenicol residue in crab by electrospray LC/MS. U. S. FDA, Lab. Inf. Bull., 19, 4294.
  13. U.S. Environmental Protection Agency. (2007) Method 1694: Pharmaceuticals and Personal Care Products in Water, Soil, Sediment, and Biosolids by HPLC/MS/MS, EPA, Washington, pp. 1-72.
  14. Imre, S., Dogaru, G., Vlase, L., Vari, C.E., Dogaru, M.T. and Caldararu, C. (2009) LC-MS Strategies in monitoring the response to different therapy. Farmacia, 57, 681-690.
  15. Cinguina, A.L., Roberti, P., Giannetti, L., Longo, F., Draisci, R., Fagiolo, A. and Brizioli, N.R. (2003) Determination of enrofloxacin and its metabolite ciprofloxacin in goat milk by high-performance liquid chromatography with diode-array detection. Optimization and validation. J. Chromatogr. A, 987, 221-226. https://doi.org/10.1016/S0021-9673(02)01800-9
  16. Lykkeberg, A.K., Halling-Sorensen, B., Cornett, C., Tiornelund, J. and Honore Hansen, S. (2004) Quantitative analysis of oxytetracycline and its impurities by LC-MS-MS. J. Pharm. Biomed. Anal., 34, 325-332. https://doi.org/10.1016/S0731-7085(03)00500-4
  17. Schneider, M.J., Mastovska, K., Lehotay, S.J., Lightfield, A.R., Kinsella, B. and Shultz, C.E. (2009) Comparison of screening methods for antibiotics in beef kidney juice and serum. Anal. Chim. Acta, 637, 290-297. https://doi.org/10.1016/j.aca.2008.08.005
  18. Bie, M., Li, R., Chai, T., Dai, S., Zhao, H., Yang, S. and Qiu, J. (2012) Simultaneous determination of tetracycline antibiotics in beehives by liquid chromatography--triple quadrupole mass spectrometry. Adv. Appl. Sci. Res., 3, 462-468.
  19. Pikkemaat, M.G., Rapallini, M.L., Karp, M.T. and Elferink, J.W. (2010) Application of a luminescent bacterial biosensor for the detection of tetracyclines in routine analysis of poultry muscle samples. Food Addit. Contam. Part A, 27, 1112-1117. https://doi.org/10.1080/19440041003794866

Cited by

  1. in an Electrospray Time-of-Flight Mass Spectrometer vol.118, pp.45, 2014, https://doi.org/10.1021/jp508059j
  2. facilitates chloramphenicol uptake into bacterial cells vol.293, pp.3, 2018, https://doi.org/10.1074/jbc.M117.805960