오공(蜈蚣) 에테르 추출물의 RAW 264.7 cell에서 LPS로 유도된 염증반응 억제 효과

Inhibitory Effect of Scolopendra Morsitans L. Ether Extract on Lipopolysaccharide-Induced Inflammatory Response in RAW 264.7 Cells

  • 정호경 (전라남도한방산업진흥원) ;
  • 조현우 (전라남도한방산업진흥원) ;
  • 정원석 (전라남도한방산업진흥원) ;
  • 최인영 (전라남도한방산업진흥원) ;
  • 조정희 (전라남도한방산업진흥원) ;
  • 정수영 (전라남도한방산업진흥원)
  • Jung, Ho Kyung (Jeonnam Development Institute for Korean Traditional Medicine) ;
  • Cho, Hyun Woo (Jeonnam Development Institute for Korean Traditional Medicine) ;
  • Jung, Won Seok (Jeonnam Development Institute for Korean Traditional Medicine) ;
  • Choi, In Young (Jeonnam Development Institute for Korean Traditional Medicine) ;
  • Cho, Jung Hee (Jeonnam Development Institute for Korean Traditional Medicine) ;
  • Jung, Su Young (Jeonnam Development Institute for Korean Traditional Medicine)
  • 투고 : 2012.10.22
  • 심사 : 2013.01.14
  • 발행 : 2013.02.25

초록

It has been reported that Scolopendra morsitans L.(SML) has beneficial effects on human health and diverse diseases. The purpose of this study was to investigate the anti-inflammatory effects of ether extract from Scolopendra morsitans L. on lipopolysaccharide(LPS)-induced inflammatory response. Thus, we examined the inhibitory effect of SML ether fraction on LPS-induced increase of inflammatory mediators(NO, iNOS, COX-2, and $I{\kappa}B{\alpha}$) and pro-inflammatory cytokines(TNF-${\alpha}$) in RAW 264.7 cells. In the present study, SML ether extract itself decreased cell viability in a dose dependent manner(> 100 ${\mu}g/ml$). In addition, LPS increased NO production, iNOS expression and phosphorylation of $I{\kappa}-B{\alpha}$, which were blocked by the treatment of SML ether fraction in a dose dependent manner. Furthermore, the treatment of LPS increased TNF-${\alpha}$ production. However, the pretreatment of SML ether fraction prevented the LPS-induced TNF-${\alpha}$ production in dose dependant manner. Taken together, our results suggest that SML may be a beneficial drug against inflammatory diseases such as sepsis.

키워드

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