Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Inhibition Effect of Taxilli Ramulus Extract on Osteoclast Differentiation and Bone Resorption

상기생 추출물이 파골세포 분화와 골흡수 억제에 미치는 효과

  • Baek, Jong Min (Department of Anatomy, Institute for Environmental Science, Wonkwang University) ;
  • Kim, Ju Young (Institute for Skeletal Disease & Imaging Science-based Lung and Bone Diseases Research Center, Wonkwang University) ;
  • Lee, Myeung Su (Institute for Skeletal Disease & Imaging Science-based Lung and Bone Diseases Research Center, Wonkwang University) ;
  • Jeung, Woo Jin (Department of Anatomy, Institute for Environmental Science, Wonkwang University) ;
  • Moon, Seo Young (Division of Anesthesiology and Pain Medicine, Sanbon Medical Center) ;
  • Jeon, Byung Hoon (Department of Pathology, College of Korean Medicine & Research Center of Traditional Korean Medicine, Wonkwang University) ;
  • Oh, Jae Min (Department of Anatomy, Institute for Environmental Science, Wonkwang University) ;
  • Choi, Min Kyu (Department of Anatomy, Institute for Environmental Science, Wonkwang University)
  • 백종민 (원광대학교 의과대학 해부학교실 & 환경과학연구소) ;
  • 김주영 (원광대학교 골격계질환연구소 및 영상기반 폐 및 골 질환 연구센터) ;
  • 이명수 (원광대학교 골격계질환연구소 및 영상기반 폐 및 골 질환 연구센터) ;
  • 정우진 (원광대학교 의과대학 해부학교실 & 환경과학연구소) ;
  • 문서영 (산본병원 마취통증의학과) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실 & 한국전통의학연구소) ;
  • 오재민 (원광대학교 의과대학 해부학교실 & 환경과학연구소) ;
  • 최민규 (원광대학교 의과대학 해부학교실 & 환경과학연구소)
  • Received : 2013.06.04
  • Accepted : 2013.07.15
  • Published : 2013.08.25
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Abstract

Bone homeostasis is maintained by co-ordination of bone-resorbing osteoclasts and bone-forming osteoblasts. Imbalance between osteoclasts and osteoblasts leads to many bone diseases such as osteoporosis, rheumatoid arthritis. Taxillus chinensis is a herb that has been widely used to improve bone health. However, the effect and mechanism of Taxillus chinensis extract on osteoclast differentiation and bone resportion has been unknown. Thus, We investigated the effect of Taxillus chinensis on expression of receptor activator of nuclear factor-${\kappa}B$ ligand (RANKL)-induced osteoclast differentiation and bone resorption. Also, the action of Taxillus chinensis on mechanisms relating to osteoclast differentiation was studied. In this results, we identified that Taxillus chinensis significantly inhibited RANKL-induced osteoclast differentiation and bone resportion. Moreover, Taxillus chinensis was suppressed the activation of NF-${\kappa}B$ in bone marrow macrophage treated RANKL and M-CSF. Taxillus chinensis was down-regulated the mRNA expression of c-Fos, nuclear factor of activated T-cells (NFAT)c1, osteoclast-associated receptor (OSCAR), tartrate-resistant acid phosphatase (TRAP). The cell adhesion-related molecules such as integrin ${\alpha}v$ and integrin ${\beta}3$, and the filamentous actin (F-actin) rings of mature osteoclasts-related molecules such as dendritic cell-specific transmembrane preotein (DC-STAMP) and cathepsin K are also suppressed. Taken together, these results indicated that Taxillus chinensis will be a good candidate to treat osteoclast-mediated bone diseases.

Keywords

References

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