The Korean Journal of Physiology and Pharmacology

  • 제17권5호
  • /
  • Pages.463-468
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  • 2013
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  • 1226-4512(pISSN)
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  • 2093-3827(eISSN)
대한약리학회 (The Korean Society of Pharmacology)
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Disappearance of Hypoxic Pulmonary Vasoconstriction and $O_2$-Sensitive Nonselective Cationic Current in Arterial Myocytes of Rats Under Ambient Hypoxia

  • Yoo, Hae Young (Department of Physiology and Ischemic/Hypoxic Disease Institute, Seoul National University College of Medicine) ;
  • Kim, Sung Joon (Department of Physiology and Ischemic/Hypoxic Disease Institute, Seoul National University College of Medicine)
  • 투고 : 2013.08.16
  • 심사 : 2013.09.30
  • 발행 : 2013.10.30
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초록

Acute hypoxia induces contraction of pulmonary artery (PA) to protect ventilation/perfusion mismatch in lungs. As for the cellular mechanism of hypoxic pulmonary vasoconstriction (HPV), hypoxic inhibition of voltage-gated $K^+$ channel (Kv) in PA smooth muscle cell (PASMC) has been suggested. In addition, our recent study showed that thromboxane $A_2$ ($TXA_2$) and hypoxia-activated nonselective cation channel ($I_{NSC}$) is also essential for HPV. However, it is not well understood whether HPV is maintained in the animals exposed to ambient hypoxia for two days (2d-H). Specifically, the associated electrophysiological changes in PASMCs have not been studied. Here we investigate the effects of 2d-H on HPV in isolated ventilated/perfused lungs (V/P lungs) from rats. HPV was almost abolished without structural remodeling of PA in 2d-H rats, and the lost HPV was not recovered by Kv inhibitor, 4-aminopyridine. Patch clamp study showed that the hypoxic inhibition of Kv current in PASMC was similar between 2d-H and control. In contrast, hypoxia and $TXA_2$-activated $I_{NSC}$ was not observed in PASMCs of 2d-H. From above results, it is suggested that the decreased $I_{NSC}$ might be the primary functional cause of HPV disappearance in the relatively early period (2 d) of hypoxia.

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참고문헌

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