DOI QR코드

DOI QR Code

Effects of TNF Secreting HEK Cells on B Lymphocytes' Apoptosis in Human Chronic Lymphocytic Leukemias

  • Valizadeh, Armita (Department of Anatomy, School of Medicine, Ahvaz Jundishapur University of Medical Sciences) ;
  • Ahmadzadeh, Ahmad (Thalassemia and Hemoglobinopathies Research Center, Shafa Hospital, Ahvaz Jundishapur University of Medical Sciences) ;
  • Teimoori, Ali (Virology Department, Ahvaz Jundishapur University of Medical Sciences) ;
  • Khodadadi, Ali (Cancer, Petroleum and Environmental Pollutants Research Center, Ahvaz Jundishapur University of Medical Sciences) ;
  • Saki, Ghasem (Department of Anatomy, School of Medicine, Ahvaz Jundishapur University of Medical Sciences)
  • 발행 : 2014.12.18

초록

Background: Tumor necrosis factor (TNF) related apoptosis-inducing ligand (TRAIL) is an antitumor candidate in cancer therapy. This study focused on effects of TRAIL, as a proapototic ligand that causes apoptosis, in B-CELL chronic lymphocytic leukemia cells (B-CLL). Materials and Methods: A population of HEK 293 cells was transducted by lentivirus that these achieved ability for producing the TRAIL protein and then HEK 293 cells transducted were placed in the vicinity of CLL cells. After 24 hours of co-culture, apoptosis of CLL cells was assessed by annexin V staining. Results: The amount of Apoptosis was examined separately in four groups: 293 HEK TRAIL ($16.17{\pm}1.04%$); 293 HEK GFP ($2.7{\pm}0.57%$); WT 293 HEK ($2{\pm}2.6%$); and CLL cells ($0.01{\pm}0.01%$). Among the groups studied, the maximum amount of apoptosis was in the group that the vector encoding TRAIL was transducted. In this group, the mean level of soluble TRAIL in the culture medium was 253pg/ml; also flow cytometry analyzes showed that proapotosis in this group was $32.8{\pm}1.6%$, which was higher than the other groups. Conclusions: In this study, we have demonstrated that TNF secreted from HEK 293 cells are effective in death of CLL cells.

키워드

참고문헌

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피인용 문헌

  1. HMGB1 translocation is involved in the transformation of autophagy complexes and promotes chemoresistance in leukaemia vol.47, pp.1, 2015, https://doi.org/10.3892/ijo.2015.2985
  2. PPI-G4 Glycodendrimers Upregulate TRAIL-Induced Apoptosis in Chronic Lymphocytic Leukemia Cells vol.17, pp.5, 2017, https://doi.org/10.1002/mabi.201600169