Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Mechanisms of Hela Cell Apoptosis Induced by Abnormal Savda Munziq Total Phenolics Combined with Chemotherapeutic Agents

  • Zhang, Yun-Xia (The 6th Department of Gynecology, the Affiliated Tumor Hospital of Xinjiang Medical University) ;
  • Abliz, Guzalnur (The 5th Department of Gynecology, the Affiliated Tumor Hospital of Xinjiang Medical University) ;
  • Ye, Wei-Jun (Department o Radiationtherapy, Sun Yat-sen University Cancer Center) ;
  • Mutalipu, Zuohelaguli (The 6th Department of Gynecology, the Affiliated Tumor Hospital of Xinjiang Medical University) ;
  • Li, Xiao-Wen (The 6th Department of Gynecology, the Affiliated Tumor Hospital of Xinjiang Medical University) ;
  • Wang, Hai-Qin (The 6th Department of Gynecology, the Affiliated Tumor Hospital of Xinjiang Medical University) ;
  • Buranjiang, Gulimire (The 6th Department of Gynecology, the Affiliated Tumor Hospital of Xinjiang Medical University) ;
  • Upur, Halmurat (Department of Drug Analysis, Xinjiang Medical University)
  • Published : 2014.01.30
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Abstract

Objective: To investigate the effects of abnormal Savda Munziq (ASMq) total phenolics combined with cisplatin and docetaxel on the Hela cell growth. Methods: In vivo cultured Hela cells were treated with cisplatin, docetaxel, total phenolics, cisplatin+total phenolics or docetaxel+total phenolics. MTT was performed to assess inhibition of cell proliferation, flow cytometry to detect apoptosis, and semi-quantitative RT-PCR to test for survivin and Bcl-2 expression. Results: The total phenolics, cisplatin and docetaxel had significant inhibitory and apoptosis-promoting effects on Hela cells (P<0.05), with the early apoptotic rates of $12.8{\pm}0.70%$, $18.9{\pm}3.79%$ and $15.8{\pm}3.8%$; the total phenolics, cisplatin and docetaxel significantly decreased the expression of Bcl-2 and survivin (all P<0.01), especially when used in combination. Conclusion: ASMq total phenolics, combined with cisplatin and docetaxel, could promote the apoptosis of Hela cells possibly through reducing the expression of Bcl-2 and survivin.

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References

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