DOI QR코드

DOI QR Code

Anticancer Properties of Teucrium persicum in PC-3 Prostate Cancer Cells

  • Tafrihi, Majid (Department of Cell and Molecular Biology, University of Tehran) ;
  • Toosi, Samane (Department of Cell and Molecular Biology, University of Tehran) ;
  • Minaei, Tayebeh (Department of Cell and Molecular Biology, University of Tehran) ;
  • Gohari, Ahmad Reza (Medicinal Plants Research Center, Tehran University of Medical Sciences) ;
  • Niknam, Vahid (Department of Botany, School of Biology, University of Tehran) ;
  • Arab Najafi, Seyed Mahmoud (Department of Cell and Molecular Biology, University of Tehran)
  • 발행 : 2014.01.30

초록

Crude extracts or phytochemicals obtained from some plants have potential anti-cancer properties. Teucrium persicum is an Iranian endemic plant belonging to the Lamiaceae family which has traditionally been used to relieve abdominal pains. However, the anti-cancer properties of this species of the Teucrium genus have not been investigated previously. In this study, we have used a highly invasive prostate cancer cell line, PC-3, which is an appropriate cell system to study anti-tumor properties of plants. A methanolic extract obtained from T persicum potently inhibited viability of PC-3 cells. The viability of SW480 colon and T47D breast cancer cells was also significantly decreased in the presence of the T persicum extract. Flow cytometry suggested that the reduction of cell viability was due to induction of apoptosis. In addition, the results of wound healing and gelatin zymography experiments supported anti-cell invasion activity of T persicum. Interestingly, sublethal concentrations of T persicum extract induced an epithelial-like morphology in a subpopulation of cells with an increase in E-Cadherin and ${\beta}$-Catenin protein levels at the cell membrane. These results strongly suggest that T persicum is a plant with very potent anti-tumor activity.

키워드

참고문헌

  1. Autore G, Capasso F, De Fusco R, et al (1984). Antipyretic and antibacterial actions of Teucrium polium (L). Pharmacol Res Commun, 16, 21-9. https://doi.org/10.1016/S0031-6989(84)80101-0
  2. Baum B, Georgiou M (2011). Dynamics of adherens junctions in epithelial establishment, maintenance, and remodeling. J Cell Biol, 192, 907-17. https://doi.org/10.1083/jcb.201009141
  3. Behrens J (1993). The role of cell adhesion molecules in cancer invasion and metastasis. Breast Cancer Res Treat, 24, 175-84. https://doi.org/10.1007/BF01833258
  4. Cakir A, Mavi A, Kazaz C, et al (2006). Antioxidant Activities of the Extracts and Components of Teucrium orientale L. var. orientale. Turk J Chem, 30, 483-94.
  5. Cavallaro U, Christofori G (2004). Cell Adhesion and Signalling by Cadherins and IG-Cams in Cancer. Nat Rev Cancer, 4, 118-32. https://doi.org/10.1038/nrc1276
  6. Chahar MK, Sharma N, Dobhal MP, et al (2011). Flavonoids: A versatile source of anticancer drugs. Pharmacogn Rev, 5, 1-12. https://doi.org/10.4103/0973-7847.79093
  7. Cragg GM, Newman DJ (2005). Plants as a source of anti-cancer agents. J Ethnopharmacol, 100, 72-9. https://doi.org/10.1016/j.jep.2005.05.011
  8. Deryugina E, Quigley JP (2006). Matrix metalloproteinases and tumor metastasis. Cancer Metastasis Rev, 25, 9-34. https://doi.org/10.1007/s10555-006-7886-9
  9. Dudley AC, Shih S-C, Cliffe AR, et al (2008). Attenuated p53 activation in tumour-associated stromal cells accompanies decreased sensitivity to etoposide and vincristine. Bri J Cancer, 99, 118-25. https://doi.org/10.1038/sj.bjc.6604465
  10. Fabricant DS, Fransworth NR (2001). The value of plants used in traditional medicine for drug discovery. Environ Health Perspect, 109, 69-75. https://doi.org/10.1289/ehp.01109s169
  11. Fukuta K, Kohri K, Fukuda H, et al (2008). Induction of multinucleated cells and apoptosis in the PC-3 prostate cancer cell line by low concentrations of polyethylene glycol 1000. Cancer Sci, 99, 1055-62. https://doi.org/10.1111/j.1349-7006.2008.00781.x
  12. Guarino M (2007). Epithelial-mesenchymal transition and tumour invasion. Int J Biochemistry Cell Biol, 39, 2153-60. https://doi.org/10.1016/j.biocel.2007.07.011
  13. Kadifkova Panovska T, Kulevanova S, Stefova M (2005). In vitro antioxidant activity of some Teucrium species (Lamiaceae). Acta Pharm, 55, 207-14.
  14. Kandouz M, Alachkar A, Zhang L, et al (2010). Teucrium polium plant extract inhibits cell invasion and motility of human prostate cancer cells via the restoration of the E-Cadherin/catenin complex. J Ethnopharmacol, 129, 410-5. https://doi.org/10.1016/j.jep.2009.10.035
  15. Kubler H, van Randenborgh H, Treiber U, et al (2005). In vitro cytotoxic effects of imatinib in combination with anticancer drugs in human prostate cancer cell lines. The Prostate, 63, 385-94. https://doi.org/10.1002/pros.20201
  16. Leckband D, Prakasam A (2006). Mechanism and dynamics of cadherin adhesion. Annu Rev Biomed Eng, 8, 259-87. https://doi.org/10.1146/annurev.bioeng.8.061505.095753
  17. Mazhar D, Waxman J (2002). Prostate cancer. Postgrad Med J, 78, 590-5. https://doi.org/10.1136/pmj.78.924.590
  18. Miri A, Monsef-Esfahani HR, Amini M, et al (2012). Comparative chemical composition and antioxidant properties of the essential oils and aromatic water from Teucrium persicum boiss. Iran J Pharm Res, 11, 573-81.
  19. Moghtader M (2009). Chemical composition of essential oil of Teucrium polium L. from Iran. American-Eurasian J Agric Environ Sci, 5, 843-6.
  20. Monsef-Esfahani HR, Miri A, Amini M, et al (2010). Seasonal variation in the chemical composition, antioxidant activity and total phenolic content of Teucrium persicum Boiss. Essential oils. Res J Biological Sciences, 5, 492-8. https://doi.org/10.3923/rjbsci.2010.492.498
  21. Nakamura T, Kato Y, Fuji H, et al (2003). E-Cadherin-dependent intercellular adhesion enhances chemoresistance. Int J Mol Med, 12, 693-700.
  22. Nirmala MJ, Samundeeswari A, Deepa Sankar P, (2011). Natural plant resources in anti-cancer therapy-A review. Res in Plant Biology, 1, 1-14.
  23. Nyska A, Suttie A, Bakshi S, et al (2003). Slowing tumorigenic progression in TRAMP mice and prostatic carcinoma cell lines using natural anti-oxidant from Spinach, NAO-A comparative study of three anti-oxidants. Toxicol Pathol, 31, 39-51. https://doi.org/10.1080/01926230390173833
  24. Polakis P (2000). Wnt signaling and cancer. Genes & Development, 14, 1837-51.
  25. Ljubuncic P, Dakwar S, Portnaya I, et al (2006). Aqueous extracts of Teucrium polium possess remarkable antioxidant activity in vitro. eCAM, 3, 329-38.
  26. Roomi MW, Monterrey JC, Kalinovsky T, et al (2009). Patterns of MMP-2 and MMP-9 expression in human cancer cell lines. Oncology Reports, 21, 1323-33.
  27. Salido M, Larran J, Lopez A, et al (1999). Etoposide sensitivity of human prostatic cancer cell lines PC-3, DU 145 and LNCaP. Histol Histopathol, 14, 125-34.
  28. Salmanian S, Najafi SMA, Rafipour M, et al (2010). Regulation of GSK-$3\beta$ and $\beta$-Catenin by Gaq in HEK293T cells. Biochem Biophys Res Commun, 14, 577-82.
  29. Schulz WA, Burchardt M, Cronauer MV (2003). Molecular biology of prostate cancer. Mol Hum Reprod, 9, 437-48. https://doi.org/10.1093/molehr/gag064
  30. Sharma G, Tyagi AK, Singh RP et al (2004). Synergistic anti-cancer effects of grape seed extract and conventional cytotoxic agent doxorubicin against human breast carcinoma cells. Breast Cancer Res and Treatment, 85, 1-12. https://doi.org/10.1023/B:BREA.0000020991.55659.59
  31. Stankovic MS, Curcic MG, Zizic JB, et al (2011). Teucrium plant species as natural sources of novel anticancer compounds: antiproliferative, proapoptotic and antioxidant properties. Int J Mol Sci, 12, 4190-205. https://doi.org/10.3390/ijms12074190
  32. Stemmler M, (2008). Cadherins in development and cancer. Molecular Biosystems, 4, 835-50. https://doi.org/10.1039/b719215k
  33. Stetler-Stevenson WG (2001). The role of matrix metalloproteinases in tumor invasion, metastasis, and angiogenesis. Surg Oncol Clin N Am, 10, 383-92.
  34. Sun W, Guo MM, Han P, et al (2012). Id-1 and the p65 subunit of NF-$\kappa{B}$ promote migration of nasopharyngeal carcinoma cells and are correlated with poor prognosis. Carcinogenesis, 33, 810-7. https://doi.org/10.1093/carcin/bgs027
  35. Tai S, Sun Y, Squires JM, et al (2011). PC3 Is a Cell line characteristic of prostatic small cell carcinoma. Prostate, 71, 1668-79. https://doi.org/10.1002/pros.21383
  36. Tang SN, Singh C, Nall D, et al (2010). The dietary bioflavonoid quercetin synergizes with epigallocathechin gallate (EGCG) to inhibit prostate cancer stem cell characteristics, invasion, migration and epithelial-mesenchymal transition. J Mol Signal, 5, 1-15. https://doi.org/10.1186/1750-2187-5-1
  37. Tariq M, Ageel A, Al-Yahya MA, et al (1989). Anti-inflammatory activity of Teucrium polium. Int J Tissue Reaction, 11, 185-8.
  38. Thiery JP (2002). Epithelial-mesenchymal transitions in tumour progression. Nature Rev Cancer, 2, 442-54. https://doi.org/10.1038/nrc822
  39. Tsakalozou E, Eckman AM, Bae Y (2012). Combination effects of docetaxel and doxorubicin in hormone-refractory prostate cancer cells. Biochemistry Res Int, 2012, 1-10.
  40. Twaij HAA, Albadr AA, Abul-Khail A (1987). Anti-ulcer activity of Teucrium polium. Pharmaceutical Biology, 25, 125-8. https://doi.org/10.3109/13880208709088138
  41. Van Roy F, Berx G (2008). The cell-cell adhesion molecule E-Cadherin. Cellular and Molecular Life Sciences, 65, 3756-88. https://doi.org/10.1007/s00018-008-8281-1
  42. Vijayababu MR, Arunkumar A, Kanagaraj P, et al (2006). Quercetin downregulates matrix metalloproteinases 2 and 9 proteins expression in prostate cancer cells (PC-3). Molecular and Cellular Biochemistry, 287, 109-16. https://doi.org/10.1007/s11010-005-9085-3
  43. Zahm JM, Kaplan H, Herard AL, et al (1997). Cell migration and proliferation during the in vitro wound repair of the respiratory epithelium. Cell Motil Cytoskeleton, 37, 33-43. https://doi.org/10.1002/(SICI)1097-0169(1997)37:1<33::AID-CM4>3.0.CO;2-I
  44. Zhang K, Waxman DJ (2010). PC3 prostate tumor-initiating cells with molecular profile FAM65Bhigh/MFI2low/LEF1low increase tumor angiogenesis. Mol Cancer, 9, 1-13.
  45. Zhou Q, Yan B, Hu X, et al (2009). Luteolin inhibits invasion of prostate cancer PC3 cells through E-Cadherin. Mol Cancer Ther, 8, 1684-91. https://doi.org/10.1158/1535-7163.MCT-09-0191

피인용 문헌

  1. Association of rs1042522 Polymorphism with Increased Risk of Prostate Adenocarcinoma in the Pakistani Population and its HuGE Review vol.15, pp.9, 2014, https://doi.org/10.7314/APJCP.2014.15.9.3973
  2. E-Cadherin/β-Catenin Complex: A Target for Anticancer and Antimetastasis Plants/Plant-derived Compounds vol.69, pp.5, 2017, https://doi.org/10.1080/01635581.2017.1320415