Asian Pacific Journal of Cancer Prevention

  • 제15권6호
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  • Pages.2447-2451
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  • 2014
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  • 1513-7368(pISSN)
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  • 2476-762X(eISSN)
아시아태평양암예방학회 (Asian Pacific Journal of Cancer Prevention)
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Cytotoxic T Lymphocytes Elicited by Dendritic Cell-Targeted Delivery of Human Papillomavirus Type-16 E6/E7 Fusion Gene Exert Lethal Effects on CaSki Cells

  • Wu, Xiang-Mei (Chongqing Molecular Medicine and Cancer Research Center, Chongqing Children's Hospital) ;
  • Liu, Xing (Department of Pediatric Urology, Chongqing Children's Hospital) ;
  • Jiao, Qing-Fang (Chongqing Molecular Medicine and Cancer Research Center, Chongqing Children's Hospital) ;
  • Fu, Shao-Yue (Chongqing Molecular Medicine and Cancer Research Center, Chongqing Children's Hospital) ;
  • Bu, You-Quan (Chongqing Molecular Medicine and Cancer Research Center, Chongqing Children's Hospital) ;
  • Song, Fang-Zhou (Chongqing Molecular Medicine and Cancer Research Center, Chongqing Children's Hospital) ;
  • Yi, Fa-Ping (Chongqing Molecular Medicine and Cancer Research Center, Chongqing Children's Hospital)
  • 발행 : 2014.03.30
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초록

Human papillomavirus (HPV) is the primary etiologic agent of cervical cancer. Consideration of safety and non human leukocyte antigen restriction, protein vaccine has become the most likely form of HPV therapeutic vaccine, although none have so far been reported as effective. Since tumor cells consistently express the two proteins E6 and E7, most therapeutic vaccines target one or both of them. In this study, we fabricated DC vaccines by transducing replication-defective recombinant adenoviruses expressing E6/E7 fusion gene of HPV-16, to investigate the lethal effects of specific cytotoxic T lymphocytes (CTL) against CaSki cells in vitro. Mouse immature dendritic cells (DC) were generated from bone marrow, and transfected with pAd-E6/E7 to prepare a DC vaccine and to induce specific CTL. The surface expression of CD40, CD68, MHC II and CD11c was assessed by flow cytometry (FCM), and the lethal effects of CTL against CaSki cells were determined by DAPI, FCM and CCK-8 methods. Immature mouse DC was successfully transfected by pAd-E6/E7 in vitro, and the transfecting efficiency was 40%-50%. A DC vaccine was successfully prepared and was used to induce specific CTL. Experimental results showed that the percentage of apoptosis and killing rate of CaSki cells were significantly increased by coculturing with the specific CTL (p <0.05). These results illustrated that a DC vaccine modified by HPV-16 E6/E7 gene can induce apoptosis of CaSki cells by inducing CTL, which may be used as a new strategy for biological treatment of cervical cancer.

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피인용 문헌

  1. TLR9 Expression in Uterine Cervical Lesions of Uyghur Women Correlate with Cervical Cancer Progression and Selective Silencing of Human Papillomavirus 16 E6 and E7 Oncoproteins in Vitro vol.15, pp.14, 2014, https://doi.org/10.7314/APJCP.2014.15.14.5867
  2. Development and Clinical Evaluation of Dendritic Cell Vaccines for HPV Related Cervical Cancer - a Feasibility Study vol.15, pp.14, 2014, https://doi.org/10.7314/APJCP.2014.15.14.5909
  3. Immunotherapeutic Effects of Dendritic Cells Pulsed with a Coden-optimized HPV 16 E6 and E7 Fusion Gene in Vivo and in Vitro vol.16, pp.9, 2015, https://doi.org/10.7314/APJCP.2015.16.9.3843
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