Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Morphological Studies on the Inhibitory Effects of Photoaging Skin of Fermented Red Ginseng in Hairless Mice

발효홍삼의 광노화 피부 억제효과에 대한 형태학적 연구

  • Lee, Chang Hyun (College of Korean Medicine, Woosuk University) ;
  • Kim, Ho Il (College of Korean Medicine, Woosuk University) ;
  • Kim, Jong Seok (Department of Rehabilitation Studies, Woosuk University) ;
  • Oh, Mi Jin (Department of Food & Biotechnology, Woosuk University) ;
  • Kim, Sun Woong (Department of Food & Biotechnology, Woosuk University) ;
  • Ma, Sang Yong (Department of Alternative Medicine, Woosuk University) ;
  • Kim, Myoung Soon (Department of Alternative Medicine, Woosuk University) ;
  • Kwon, Jin (Department of Prosthetics and Orthotics, Korea National College of Welfare) ;
  • Jeong, Han Sol (Division of Applied Medicine, School of Korean Medicine, Pusan National University) ;
  • Oh, Chan Ho (Department of Food & Biotechnology, Woosuk University)
  • 이창현 (우석대학교 한의과대학) ;
  • 김호일 (우석대학교 한의과대학) ;
  • 김종석 (우석대학교 재활학과) ;
  • 오미진 (우석대학교 식품생명공학과) ;
  • 김선웅 (우석대학교 식품생명공학과) ;
  • 마상용 (우석대학교 대체의학과) ;
  • 김명순 (우석대학교 대체의학과) ;
  • 권진 (한국복지대학교 의료보장구과) ;
  • 정한솔 (부산대학교 한의학전문대학원 응용의학부) ;
  • 오찬호 (우석대학교 식품생명공학과)
  • Received : 2014.01.02
  • Accepted : 2014.02.07
  • Published : 2014.04.25
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Abstract

To investigate the anti-photoaging effect of fermented Red Ginseng(RG) in SKH-1 mice. We examined the effects of extracts of non-fermented RG(NRG group), fermented RG(FRG group) and fortified fermented RG(FFRG group) on skin wrinkles formation, histological changes related to the number of epidermal cell layers, epidermal thickness, neutrophil infiltration into dermis, degradation of collagen fibers, and the number of mast cells, and immunohistochemical changes related to cytokines and enzymes in photoaging skin caused by UVB irradiation of SKH-1 mice. The oral administration(300 mg/Kg B.W./day) and topical application($100{\mu}{\ell}/mouse/day$) of extracts of NRG, FRG and FFRG inhibited increases in epidermal thickness and wrinkle formation compared to control group in dorsal skin induced by UVB irradiation. We observed more increased stainability of acid fuschin and aniline blue in dermis of FFRG group than those of other groups. Furthermore, NRG, FRG and FFRG prevented the disruption of collagen fibers within papillary layer of dermis, and decreased number of mast cells in the dorsal skins induced by UVB irradiation. We observed fine wrinkle formation in FFRG group. Treatment with NRG, FRG and FFRG decreased immunohistochemical density of myeloperoxidase related to inflammation in the photoaging skin. We observed more decreased immunohistochemical density of myeloperoxidase in FFRG group than those of other groups. Immunohistochemical density of PCNA and Ki-67 in FFRG group was more decreased than those of other groups. Our study suggests that fermented red ginseng extracts participates in inhibitory effects in the morphological processes related to photoaging skin on UVB irradiated SKH-1 mice.

Keywords

References

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