Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effects of Hoesaeng-san Ethanol Extract on the Human Mast cell-mediated Inflammatory Responses

회생산(回生散) 에탄올 추출물이 비만세포 매개성 염증반응에 미치는 영향

  • Park, Jee Hea (Department of Third Medicine, Professional Graduate School of Korean Medicine, Wonkwang University) ;
  • Kwon, Dong Yeol (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University) ;
  • Lee, Su Kyung (Department of Oriental Rehabilitation Medicine, College of Korean Medicine, Wonkwang University)
  • 박지혜 (원광대학교 한의학전문대학원 제3의학과) ;
  • 권동렬 (원광대학교 약학대학 한약학과) ;
  • 이수경 (원광대학교 한의과대학 한방재활의학과학교실)
  • Received : 2013.11.25
  • Accepted : 2014.02.05
  • Published : 2014.02.25
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Abstract

Hoesaeng-san is known to be effective for treating diarrhea and vomiting. However the therapeutic mechanism of Hoesaeng-san is still not well understood. The aim of the present study was to demonstrate the effects of Hoesaeng-san ethanol extract (HSSEE) on the expression of pro-inflammatory cytokines, as well as to elucidate its mechanism of action in the human mast cell line (HMC-1). Mast Cell were stimulated with phorbol 12-myristate 13-acetate (PMA) plus A23187 in the presence or absence of HSSEE. To study the possible effects of HSSEE, ELISA, RT-PCR, Western blot analysis were used in this study. HSSEE significantly inhibited the PMA plus A23187-induction of inflammatory cytokines such as tumor necrosis factor (TNF)-${\alpha}$, interleukin (IL)-6 and IL-8. In activated HMC-1 cells, phosphorylation of extra-signal response kinase (ERK) 1/2 and c-jun n-terminal kinase (JNK)1/2 decreased after treatment with HSSEE. Moreover HSSEE inhibited PMA plus A23187-induced nuclear factor (NF)-${\kappa}B$ activation and $I{\kappa}B$ degradation. HSSEE suppressed the expression of TNF-${\alpha}$, IL-6, IL-8 through a decrease in the ERK 1/2 and JNK 1/2, as well as activation of NF-${\kappa}B$. These results indicated that HSSEE exerted a regulatory effect on inflammatory reactions mediated by mast cells.

Keywords

References

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