Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
권호 표지
DOI QR코드

DOI QR Code

Cecropin Suppresses Human Hepatocellular Carcinoma BEL-7402 Cell Growth and Survival in vivo without Side-Toxicity

  • Jin, Xiao-Bao (School of Basic Courses, Guangdong Pharmaceutical University) ;
  • Wang, Ying-Jiao (School of Basic Courses, Guangdong Pharmaceutical University) ;
  • Liang, Lu-Lu (School of Basic Courses, Guangdong Pharmaceutical University) ;
  • Pu, Qiao-Hong (School of Basic Courses, Guangdong Pharmaceutical University) ;
  • Shen, Juan (School of Basic Courses, Guangdong Pharmaceutical University) ;
  • Lu, Xue-Mei (School of Basic Courses, Guangdong Pharmaceutical University) ;
  • Chu, Fu-Jiang (School of Basic Courses, Guangdong Pharmaceutical University) ;
  • Zhu, Jia-Yong (School of Basic Courses, Guangdong Pharmaceutical University)
  • Published : 2014.07.15
Download PDF
⟨ Previous Next ⟩

Abstract

Conventional chemotherapy against hepatocellular carcinoma typically causes various side effects. Our previous study showed that cecropin of Musca domestica can induce apoptosis in human hepatocellular carcinoma BEL-7402 cells in vitro. However, whether cecropin inhibits BEL-7402 cell in vivo and the question of possible side effects remained undentified. The present study confirmed tumor-inhibitory effects of cecropin in vivo, and furthermore strongly suggested that cecropin cytotoxicity in BEL-7402 cells in vivo may be mainly derived from its pro-apoptotic action. Specifically, we found that cecropin exerted no obvious side effects in tumor-bearing mice as it had no significant hematoxicity as well as visceral toxicity. Therefore, cecropin may be a potential candidate for further investigation as an antitumor agent against hepatocellular carcinoma.

Keywords

References

  1. Ceron JM, Contreras-Moreno J, Puertollano E, et al (2010). The antimicrobial peptide cecropin A induces caspase-independent cell death in human promyelocytic leukemia cells. Peptides, 31, 1494-503. https://doi.org/10.1016/j.peptides.2010.05.008
  2. Chan SC, Hui L, Chen HM (1998). Enhancement of the cytolytic effect of anti-bacterial cecropin by the microvilli of cancer cells. Anticancer Res, 18, 4467-74.
  3. Chen J, Lan T, Hou J, et al (2012). Atorvastatin sensitizes human non-small cell lung carcinomas to carboplatin via suppression of AKT activation and upregulation of TIMP-1. Int J Biochem Cell Biol, 44, 759-69. https://doi.org/10.1016/j.biocel.2012.01.015
  4. Fatemeh Zolfagharzadeh, Valiollah Dabidi Roshan (2013). Pretreatment hepatoprotective effect of regular aerobic training against hepatic toxicity induced by doxorubicin in rats. Asian Pac J Cancer Prev, 14, 2931-36. https://doi.org/10.7314/APJCP.2013.14.5.2931
  5. Giglia JL, Antonia SJ, Berk LB, et al (2010). Systemic therapy for advanced hepatocellular carcinoma: past, present, and future. Cancer Control, 17, 120-9. https://doi.org/10.1177/107327481001700207
  6. Gown AM, Willingham MC (2002). Improved detection of apoptotic cells in archival paraffin sections: immunohistochemistry using antibodies to cleaved caspase 3. J Histochem Cytochem, 50, 449-54. https://doi.org/10.1177/002215540205000401
  7. Guani-Guerra E, Santos-Mendoza T, Lugo-Reyes SO, Teran LM (2010). Antimicrobial peptides: general overview and clinical implications in human health and disease. Clin Immunol, 135, 1-11. https://doi.org/10.1016/j.clim.2009.12.004
  8. Huang SM, Chien LY, Tai CJ, et al (2013). Effectiveness of 3-week intervention of Shi Quan Da Bu Tang for alleviating hematotoxicity among patients with breast carcinoma receiving chemotherapy. Integr Cancer Ther, 12, 136-44. https://doi.org/10.1177/1534735412450513
  9. Injac R, Perse M, Obermajer N, et al (2008). Potential hepatoprotective effects of fullerenol C60 (OH)24 in doxorubicin-induced hepatotoxicity in rats with mammary carcinomas. Biomaterials, 29, 3451-60. https://doi.org/10.1016/j.biomaterials.2008.04.048
  10. Jin X, Mei H, Li X, et al (2010). Apoptosis-inducing activity of the antimicrobial peptide cecropin of Musca domestica in human hepatocellular carcinoma cell line BEL-7402 and the possible mechanism. Acta Biochim Biophys Sin, 42, 259-65. https://doi.org/10.1093/abbs/gmq021
  11. Jin XB, Li XB, Zhu JY, et al (2011). The target of Musca domestica cecropin on human hepatocellular carcinoma BEL-7402 cells. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi, 29, 271-3.
  12. Jin XB, Mei HF, Pu QH, et al (2013). Effects of Musca domestica cecropin on the adhesion and migration of human hepatocellular carcinoma BEL-7402 cells. Biol Pharm Bull, 36, 938-43. https://doi.org/10.1248/bpb.b12-00935
  13. Jin XB, Zhu JY, Ma Y, Liu LS (2007). The expression of antibacterial peptide cecropin gene in COS-7 cells and the preliminary study on the activities of its gene product. Chin J Zoonoses, 23, 566-8.
  14. Khynriam D, Prasad SB (2001). Hematotoxicity and blood glutathione levels after cisplatin treatment of tumor-bearing mice. Cell Biol Toxicol, 17, 357-70. https://doi.org/10.1023/A:1013784115683
  15. Liang Y, Wang JX, Zhao XF, et al (2006). Molecular cloning and characterization of cecropin from the housefly (Musca domestica), and its expression in Escherichia coli. Dev Comp Immunol, 30, 249-57. https://doi.org/10.1016/j.dci.2005.04.005
  16. Lin H, de Stanchina E, Zhou XK, et al (2010). Maitake beta-glucan promotes recovery of leukocytes and myeloid cell function in peripheral blood from paclitaxel hematotoxicity. Cancer Immunol Immunother, 59, 885-97. https://doi.org/10.1007/s00262-009-0815-3
  17. Moore AJ, Devine DA, Bibby MC (1994). Preliminary experimental anticancer activity of cecropins. Pept Res, 7, 265-9.
  18. Schweizer F (2009). Cationic amphiphilic peptides with cancer-selective toxicity. Eur J Pharmacol, 625, 190-4. https://doi.org/10.1016/j.ejphar.2009.08.043
  19. Sener SF, Grey N (2005). The global burden of cancer. J Surg Oncol, 92, 1-3. https://doi.org/10.1002/jso.20335
  20. Somboon K, Siramolpiwat S, Vilaichone R-K (2014). Epidemiology and survival of hepatocellular carcinoma in the central region of Thailand. Asian Pac J Cancer Prev, 15, 3567-70. https://doi.org/10.7314/APJCP.2014.15.8.3567
  21. Sostelly A, Henin E, Chauvenet L, et al (2013). Can we predict chemo-induced hematotoxicity in elderly patients treated with pegylated liposomal doxorubicin? Results of a population-based model derived from the DOGMES phase II trial of the GINECO. J Geriatr Oncol, 4, 48-57. https://doi.org/10.1016/j.jgo.2012.06.004
  22. Steiner H, Hultmark D, Engstrom A, et al (1981). Sequence and specificity of two antibacterial proteins involved in insect immunity. Nature, 292, 246-8. https://doi.org/10.1038/292246a0
  23. Suttmann H, Retz M, Paulsen F, et al (2008). Antimicrobial peptides of the Cecropin-family show potent antitumor activity against bladder cancer cells. BMC Urol, 8, 1-7. https://doi.org/10.1186/1471-2490-8-1

Cited by

  1. Cecropin-P17, an analog of Cecropin B, inhibits human hepatocellular carcinoma cell HepG-2 proliferation via regulation of ROS, Caspase, Bax, and Bcl-2 vol.21, pp.8, 2015, https://doi.org/10.1002/psc.2786
  2. CecropinXJ inhibits the proliferation of human gastric cancer BGC823 cells and induces cell death in vitro and in vivo vol.46, pp.5, 2015, https://doi.org/10.3892/ijo.2015.2933
  3. Tumor cell membrane-targeting cationic antimicrobial peptides: novel insights into mechanisms of action and therapeutic prospects vol.74, pp.20, 2017, https://doi.org/10.1007/s00018-017-2604-z
  4. The antibacterial peptide from Bombyx mori cecropinXJ induced growth arrest and apoptosis in human hepatocellular carcinoma cells vol.12, pp.1, 2016, https://doi.org/10.3892/ol.2016.4601
  5. Silkworm Pupa Protein Hydrolysate Induces Mitochondria-Dependent Apoptosis and S Phase Cell Cycle Arrest in Human Gastric Cancer SGC-7901 Cells vol.19, pp.4, 2018, https://doi.org/10.3390/ijms19041013