DOI QR코드

DOI QR Code

Increased Argonaute 2 Expression in Gliomas and its Association with Tumor Progression and Poor Prognosis

  • Feng, Bo (Department of Neurology, Hospital Affiliated to Binzhou medical University) ;
  • Hu, Peng (Department of Spine, Hospital Affiliated to Binzhou medical University) ;
  • Lu, Shu-Jun (Department of Neurology, Hospital Affiliated to Binzhou medical University) ;
  • Chen, Jin-Bo (Department of Neurology, Hospital Affiliated to Binzhou medical University) ;
  • Ge, Ru-Li (Department of Neurology, Hospital Affiliated to Binzhou medical University)
  • 발행 : 2014.05.15

초록

Background: Previous studies have showed that argonaute 2 is a potential factor related to genesis of several cancers, however, there have been no reports concerning gliomas. Methods: Paraffin specimens of 129 brain glioma cases were collected from a hospital affiliated to Binzhou Medical University from January 2008 to July 2013. We examined both argonaute 2 mRNA and protein expression by real-time quantitative PCR (qRT-PCR), Western blot analysis, and immunohistochemistry (IHC). The survival curves of the patients were determined using the Kaplan-Meier method and Cox regression, and the log-rank test was used for statistical evaluations. Results: Both argonaute 2 mRNA and protein were upregulated in high-grade when compared to low-grade tumor tissues. Multivariate analysis revealed that argonaute 2 protein expression was independently associated with the overall survival (HR=4.587, 95% CI: 3.001-6.993; P=0.002), and that argonaute 2 protein expression and WHO grading were independent prognostic factors for progression-free survival (HR=4.792, 95% CI: 3.993-5.672; P<0.001, and HR=2.109, 95% CI: 1.278-8.229; P=0.039, respectively). Kaplan-Meier analysis with the log-rank test indicated that high argonaute 2 protein expression had a significant impact on overall survival (P=0.0169) and progression-free survival (P=0.0324). Conclusions: The present study showed that argonaute 2 expression is up-regulated in gliomas. Argonaute 2 might also serve as a novel prognostic marker.

키워드

참고문헌

  1. Arshad H, Ahmad Z, Hasan SH (2010). Gliomas: correlation of histologic grade, Ki67 and p53 expression with patient survival. Asian Pac J Cancer Prev, 11, 1637-40.
  2. Chen DQ, Yao DX, Zhao HY, et al (2012). DNA repair gene ERCC1 and XPD polymorphisms predict glioma susceptibility and prognosis. Asian Pac J Cancer Prev, 13, 2791-4. https://doi.org/10.7314/APJCP.2012.13.6.2791
  3. Cheng N, Li Y, Han ZG (2013). Argonaute2 promotes tumor metastasis by way of up-regulating focal adhesion kinase expression in hepatocellular carcinoma. Hepatology, 57, 1906-18. https://doi.org/10.1002/hep.26202
  4. Dellaretti M, Reyns N, Touzet G, et al (2012). Diffuse brainstem glioma: prognostic factors. J Neurosurg, 117, 810-4. https://doi.org/10.3171/2012.7.JNS111992
  5. Demuth T, Berens ME (2004). Molecular mechanisms of glioma cell migration and invasion. J Neurooncol, 70, 217-28. https://doi.org/10.1007/s11060-004-2751-6
  6. Farazi TA, Hoell JI, Morozov P, et al (2013). MicroRNAs in human cancer. Adv Exp Med Biol, 774, 1-20. https://doi.org/10.1007/978-94-007-5590-1_1
  7. Johnson DR, Galanis E (2013). Incorporation of prognostic and predictive factors into glioma clinical trials. Curr Oncol Rep, 15, 56-63. https://doi.org/10.1007/s11912-012-0279-z
  8. Li W, Liu M, Feng Y, et al (2013). Evaluation of Argonaute protein as a predictive marker for human clear cell renal cell carcinoma. Int J Clin Exp Pathol, 6, 1086-94.
  9. Maqbool R, Hussain MU (2014). MicroRNAs and human diseases: diagnostic and therapeutic potential. Cell Tissue Res.
  10. O'Carroll D, Mecklenbrauker I, Das PP, et al (2007). A Slicer-independent role for Argonaute 2 in hematopoiesis and the microRNA pathway. Genes Dev, 21, 1999-2004. https://doi.org/10.1101/gad.1565607
  11. Plummer PN, Freeman R, Taft RJ, et al (2013). MicroRNAs regulate tumor angiogenesis modulated by endothelial progenitor cells. Cancer Res, 73, 341-52.
  12. Sasaki T, Shiohama A, Minoshima S, et al (2003). Identification of eight members of the Argonaute family in the human genome small star, filled. Genomics, 82, 323-30. https://doi.org/10.1016/S0888-7543(03)00129-0
  13. Schwarzenbach H, Nishida N, Calin GA, et al (2014). Clinical relevance of circulating cell-free microRNAs in cancer. Nat Rev Clin Oncol.
  14. Wang JB, Dong DF, Wang MD, et al (2014). IDH1 overexpression induced chemotherapy resistance and IDH1 mutation enhanced chemotherapy sensitivity in glioma cells in vitro and in vivo. Asian Pac J Cancer Prev, 15, 427-32. https://doi.org/10.7314/APJCP.2014.15.1.427
  15. Yang FQ, Huang JH, Liu M, et al (2013). Argonaute 2 is up-regulated in tissues of urothelial carcinoma of bladder. Int J Clin Exp Pathol, 7, 340-7.
  16. Ye S, Rong J, Huang SH, et al (2012). XRCC1 and ADPRT polymorphisms associated with survival in breast cancer cases treated with chemotherapy. Asian Pac J Cancer Prev, 13, 4923-6. https://doi.org/10.7314/APJCP.2012.13.10.4923
  17. Yoo NJ, Hur SY, Kim MS, et al (2010). Immunohistochemical analysis of RNA-induced silencing complex-related proteins AGO2 and TNRC6A in prostate and esophageal cancers. APMIS, 118, 271-6. https://doi.org/10.1111/j.1600-0463.2010.02588.x
  18. Zhang J, Fan XS, Wang CX, et al (2013a). Up-regulation of Ago2 expression in gastric carcinoma. Med Oncol, 30, 628. https://doi.org/10.1007/s12032-013-0628-2
  19. Zhang R, Su B (2009). Small but influential: the role of microRNAs on gene regulatory network and 3'UTR evolution. J Genet Genomics, 36, 1-6. https://doi.org/10.1016/S1673-8527(09)60001-1
  20. Zhang X, Graves P, Zeng Y (2013b). Overexpression of human Argonaute2 inhibits cell and tumor growth. Biochim Biophys Acta, 1830, 2553-61. https://doi.org/10.1016/j.bbagen.2012.11.013
  21. Zhou X, Guo H, Chen K, et al (2010). Identification, chromosomal mapping and conserved synteny of porcine Argonaute family of genes. Genetica, 138, 805-12. https://doi.org/10.1007/s10709-010-9462-z

피인용 문헌

  1. High Expression of Forkhead Box Protein C2 is Related to Poor Prognosis in Human Gliomas vol.15, pp.24, 2015, https://doi.org/10.7314/APJCP.2014.15.24.10621
  2. Expression of p16 and Survivin in gliomas and their correlation with cell proliferation pp.1792-1082, 2015, https://doi.org/10.3892/ol.2015.3180