Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effect of Ethanolic Extract of Silkworm on Reactive Oxygen Species Formation in vivo

누에의 에탄올 추출물이 체내 활성산소 생성에 미치는 영향

  • Kang, Young Kook (College of Liberal Arts, Daejeon University) ;
  • Choi, Min Joo (Department of Biology, College of Natural Science, Daejeon University) ;
  • Nam, Sang Ho (Department of Biology, College of Natural Science, Daejeon University)
  • 강영국 (대전대학교 교양학부대학) ;
  • 최민주 (대전대학교 자연과학대학 생명과학과) ;
  • 남상호 (대전대학교 자연과학대학 생명과학과)
  • Received : 2014.07.16
  • Accepted : 2014.08.21
  • Published : 2014.08.25
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study is to investigate the effects of silkworm extracts (SWE) on reactive oxygen species formation in mice (C57BL/6). Mice were administrated intraperitoneally with SWE (20 mg/kg/day) for 14 days. All animals were sacrificed 24 hours after the last SWE treatment and then extracted the blood and brain tissue in mouse. The researcher measured several parameters related to reactive oxygen species formation, malondialdehyde (MDA) and hydrogen peroxide ($H_2O_2$) contents in serum, whole brain, cerebral cortex and cerebellum. The results showed that MDA content of pre-SWE treatment was decreased significantly in serum, mitochondrial and cytosolic fraction of whole brain and cerebellum (P<0.01). The $H_2O_2$ content of pre-SWE treatment was decreased significantly in mitochondrial fraction of whole brain, cerebral cortex and cerebellum (P<0.01). These results suggest that SWE plays an important role for inhibition of oxidative damage of cells as well as antioxidant effect, aging delay and cells protected from irradiation.

Keywords

References

  1. 최병기, 정세영, 박광식, 조정희. 활성산소와 질환. 서울. 신일 상사출판사. pp 7-15, 2004.
  2. Fridovich, I. Biological effects of the superoxide radical. Arch Biochem Biophys. 247: 1-15, 1986. https://doi.org/10.1016/0003-9861(86)90526-6
  3. Miquel, J. Historical introduction to free radical and antioxidant biomedical research. CRC Press. Florida. 1: 3-16, 1989.
  4. Choi, J.H., Kim, D.I., Park, S.H., Back, S.J., Kim, N.J., Ryu, K.S. Development of anti-diabetes drink using with silkworm(Bombyx mori) extract. Kor J Seric Sci. 45(2):96-102, 2003.
  5. 김창효. 산업곤충. 진주. 경상대학교출판부. pp 23-28, 1995.
  6. 남중희, 마영일. 여러 나라 곤충의 자원화와 그 이용. 서울. 서울대학교 출판부. p 108, 176, 2010.
  7. Chung, S.H., Ryu, J.H., Kim, I.J. Blood glucose lowering of silkworm. J HyungHee Pharm. 24: 95-100, 1996.
  8. Cho, M.R., Choue, R.W., Chung, S.H., Ryu, J.W. Effects of silkworm powder on blood glucose and lipid levels in NIDDM (type-II) patients. Kor J Nutr 31(7):1139-1150, 1998.
  9. Ryu, K.S., Lee, H.S., Chung, S.H., Kang, P.D. An activity of lowering blood-glucose levels according to preparative conditions of silkworm power. Kor J Seric Sci. 39(1):79-85, 1997.
  10. Kim, S.H., Kim, K.S., Lee, J.H., Chung, E.K., Park, Y.S., Park, Y.J., Lee, H.Y. Comparison of glucose-lowering activity of the extracts from Kangwon-do mountain mulberry leaves(Moli folium) and silkworm. Kor J Appl Microbiol Biotechnol. 25(4):391-395, 1997.
  11. Kim, M.S., Choue, R.W., Chung, S.H., Koo, S.J. Blood glucose lowering effects mulberry leaves and silkworm extracts an mice fed with high-carbohydrate diet. Kor Nutr Society. 31(2): 117-125, 1998.
  12. Kang, Y.K., Choo, S.K., Nam, S.H. Effects of silkwormextract on blood components levels in mice. Kor J Entomol. 31(4):243-247, 2001.
  13. Kang, Y.K., Lim, H.B., Sohn, H.O., Lee, Y.G., Lee, D.W., Nam, S.H. Effect of silkworm-extract on mucus secretion in rat tracheobronchial lumen. Kor J Entomol. 30(2): 71-75, 2000.
  14. Kang, Y.K., Nam, S.H., Sohn, H.O., Lee, D.W. Inhibitory effects of silkworm-extract (SE) on monoamine oxidase activity in vitro and in vivo. Entomol Res. 35(3):189-193, 2005. https://doi.org/10.1111/j.1748-5967.2005.tb00158.x
  15. Kang, Y.K., Oh, H.S., Cho, Y.H., Kim, Y.J., Han, Y.G., Nam, S.H. Effects of a silkworm extract on dopamine and monoamine oxidase-B activity in an MPTP-induced Parkinson's disease model. Lab Anim Res. 26(3):287-292, 2010. https://doi.org/10.5625/lar.2010.26.3.287
  16. Kim, E.J., Kim, S.H., Kim, S.M. Development of a beverage using the extracts from Bombyx mori L., Morus alba L., Dioscoreae rhuzome and Inonotus obliquus. Kor J Food Preserv. 18(6):844-852, 2011. https://doi.org/10.11002/kjfp.2011.18.6.844
  17. Choi, J.H., Kim, D.I., Park, S.H., Back, S.J., Kim, N.J., Ryu, K.S. Development of anti-diabetes drink using with silkworm (Bombyx mori L.) extract. Kor J Seric Sci. 45(2):96-102, 2003.
  18. Lowry, O.H., Rosebrough, H.J., Farr, A.L., Randall, R.J. Protein measurment with the Folin-phenol reagent. J Biol Chem. 193: 265-275, 1951.
  19. Suematsu, T., Kamada, T., Abe, H., Kikuchi, S., Yagi, K. Serum lipoperoxide levels in patients suffering from liver disease. Clin Chem Acta. 79: 267-270, 1977. https://doi.org/10.1016/0009-8981(77)90486-7
  20. Cathcart, R., Schwiers, E., Ames, B.N. Detection of picomole levels hydroperoxides using a fluorescent dichlorofluorescein assay. Anal Biochem. 134: 111-116, 1983. https://doi.org/10.1016/0003-2697(83)90270-1
  21. Van Birgelen, A.P.J.M., Hebert, C.D., Wenk, M.L., Grimes, L.K., Chapin, R.E., Mahler, J., Travlos, G.S., Bucher, J.R. Toxicity of 3,3,4,4-tetrachloroazobenzene in rats and mice. Toxicol and Appl Pharmacol. 156(2):147-159, 1999. https://doi.org/10.1006/taap.1999.8640
  22. Walker, N.J., Tritscher, A.M., Sills, R.C., Lucier, G.W., Portier, C.J. Hepatocarcinogenesis in female Sprague-Dawley rata following discontinuous treatment with 2,3,7,8- etrachlorodibenzo-p-dioxin. Toxicol Sci. 54(2):330-337, 2000. https://doi.org/10.1093/toxsci/54.2.330
  23. 류강선. 우리들은 당뇨병을 극복했다. 서울. 오성출판사. pp 52-58, 2009.
  24. Knopowski, J., Wieczorowska-Tobis, K., Witowski, J. Phthophysiology of aging. J physiol Phamacol. 53(2):135-146, 2002.
  25. Martin, G.M., Austad, S.N., Johnson, T.E. Genetic analysis of aging: Role of oxidative damage and environmental stress. Nature Genetics. 13: 25-34, 1966.
  26. Berlett, S.S., Stadtman, E.R. Protein oxidation in aging, disease and oxidative stress. J Biol Chem. 272: 20313-20316, 1997. https://doi.org/10.1074/jbc.272.33.20313
  27. Finkel, T., Halbrook, N.J. Oxidants, oxidative stress and the biology of aging. Nature 408: 239-247, 2000. https://doi.org/10.1038/35041687
  28. Black, M.J. and Brandt, R.B. Spectrofluorometric analysis of hydrogen peroxide. Anal Biochem. 58: 246-254, 1974. https://doi.org/10.1016/0003-2697(74)90464-3
  29. Keston, A.S., Brandt, R.B. The fluorometric analysis of ultramicro quantities of hydrogen peroxide. Anal Biochem. 11: 1-5, 1965. https://doi.org/10.1016/0003-2697(65)90034-5