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Parecoxib: an Enhancer of Radiation Therapy for Colorectal Cancer

  • Xiong, Wei (Department of Radiation Oncology, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University) ;
  • Li, Wen-Hui (Department of Radiation Oncology, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University) ;
  • Jiang, Yong-Xin (Cancer Research Institute of Yunnan Province, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University) ;
  • Liu, Shan (Cancer Research Institute of Yunnan Province, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University) ;
  • Ai, Yi-Qin (Department of Radiation Oncology, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University) ;
  • Liu, Rong (Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology) ;
  • Chang, Li (Department of Radiation Oncology, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University) ;
  • Zhang, Ming (Department of Radiation Oncology, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University) ;
  • Wang, Xiao-Li (Department of Radiation Oncology, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University) ;
  • Bai, Han (Department of Radiation Oncology, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University) ;
  • Wang, Hong (Cancer Research Institute of Yunnan Province, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University) ;
  • Zheng, Rui (Cancer Research Institute of Yunnan Province, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University) ;
  • Tan, Jing (Department of Radiation Oncology, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University)
  • 발행 : 2015.02.25

초록

Background: To study the effect of parecoxib, a novel cyclooxygenase-2 selective inhibitor, on the radiation response of colorectal cancer (CRC) cells and its underlying mechanisms. Materials and Methods: Both in vitro colony formation and apoptosis assays as well as in vivo mouse xenograft experiments were used to explore the radiosensitizing effects of parecoxib in human HCT116 and HT29 CRC cells. Results: Parecoxib sensitized CRC cells to radiation in vitro with a sensitivity enhancement ratio of 1.32 for HCT116 cells and 1.15 for HT29 cells at a surviving fraction of 0.37. This effect was partially attributable to enhanced apoptosis induction by parecoxib combined with radiation, as illustrated using an in vitro apoptosis assays. Parecoxib augmented the tumor response of HCT116 xenografts to radiation, achieving growth delay more than 20 days and an enhancement factor of 1.53. In accordance with the in vitro results, parecoxib combined with radiation resulted in less proliferation and more apoptosis in tumors than radiation alone. Radiation monotherapy decreased microvessel density (MVD) and microvessel intensity (MVI), but increased the hypoxia level in xenografts. Parecoxib did not affect MVD, but it increased MVI and attenuated hypoxia. Conclusions: Parecoxib can effectively enhance radiation sensitivity in CRC cells through direct effects on tumor cells and indirect effects on tumor vasculature.

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참고문헌

  1. Armulik A, Abramsson A, Betsholtz C (2005). Endothelial/pericyte interactions. Circ Res, 97, 512-23. https://doi.org/10.1161/01.RES.0000182903.16652.d7
  2. Birbrair A, Zhang T, Wang ZM, et al (2015). Pericytes at the intersection between tissue regeneration and pathology. Clin Sci (Lond), 128, 81-93. https://doi.org/10.1042/CS20140278
  3. Chang L, Liu YY, Zhu B, et al (2009). High expression of the circadian gene mPer2 diminishes the radiosensitivity of NIH 3T3 cells. Braz J Med Biol Res, 42, 882-91. https://doi.org/10.1590/S0100-879X2009005000022
  4. Chen FH, Chiang CS, Wang CC, et al (2009). Radiotherapy decreases vascular density and causes hypoxia with macrophage aggregation in TRAMP-C1 prostate tumors. Clin Cancer Res, 15, 1721-9. https://doi.org/10.1158/1078-0432.CCR-08-1471
  5. Dannenberg AJ, Subbaramaiah K (2003). Targeting cyclooxygenase-2 in human neoplasia: rationale and promise. Cancer Cell, 4, 431-6. https://doi.org/10.1016/S1535-6108(03)00310-6
  6. Debucquoy A, Goethals L, Geboes K, et al (2006). Molecular responses of rectal cancer to preoperative chemoradiation. Radiother Oncol, 80, 172-7. https://doi.org/10.1016/j.radonc.2006.07.016
  7. de Lussanet QG, Backes WH, Griffioen AW, et al (2005). Dynamic contrast-enhanced magnetic resonance imaging of radiation therapy-induced microcirculation changesin rectal cancer. Int J Radiat Oncol Biol Phys, 63, 1309-15. https://doi.org/10.1016/j.ijrobp.2005.04.052
  8. Dou X, Wang RB, Yan HJ, et al (2013). Circulating lymphocytes as predictors of sensitivity to preoperative chemoradiotherapy in rectal cancer cases. Asian Pac J Cancer Prev, 14, 3881-5. https://doi.org/10.7314/APJCP.2013.14.6.3881
  9. Eberstal S, Badn W, Fritzell S, et al (2012). Inhibition of cyclooxygenase-2 enhances immunotherapy against experimental brain tumors. Cancer Immunol Immunother, 61, 1191-9. https://doi.org/10.1007/s00262-011-1196-y
  10. Harris RE, Casto BC, Harris ZM (2014). Cyclooxygenase-2 and the inflammogenesis of breast cancer. World J Clin Oncol, 5, 677-92. https://doi.org/10.5306/wjco.v5.i4.677
  11. Khan Z, Khan N, Tiwari RP, et al (2011). Biology of Cox-2: an application in cancer therapeutics. Curr Drug Targets, 12, 1082-93. https://doi.org/10.2174/138945011795677764
  12. Kiguchi K, Ruffino L, Kawamoto T, et al (2007). Therapeutic effect of CS-706, a specific cyclooxygenase-2 inhibitor, on gallbladder carcinoma in BK5.ErbB-2 mice. Mol Cancer Ther, 6, 1709-17. https://doi.org/10.1158/1535-7163.MCT-07-0015
  13. Kim NK, Baik SH, Seong JS, et al (2006). Oncologic outcomes after neoadjuvant chemoradiation followed by curative resection with tumor-specific mesorectal excision for fixed locally advanced rectal cancer: Impact of postirradiated pathologic downstaging on local recurrence and survival. Ann Surg, 244, 1024-30. https://doi.org/10.1097/01.sla.0000225360.99257.73
  14. Kim YM, Pyo H (2013). Different cell cycle modulation by celecoxib at different concentrations. Cancer Biother Radiopharm, 28, 138-45. https://doi.org/10.1089/cbr.2012.1264
  15. Kirane A, Toombs JE, Larsen JE, et al (2012a). Epithelialmesenchymal transition increases tumor sensitivity to COX-2 inhibition by apricoxib. Carcinogenesis, 33, 1639-46. https://doi.org/10.1093/carcin/bgs195
  16. Kirane A, Toombs JE, Ostapoff K, et al (2012b). Apricoxib, a novel inhibitor of COX-2, markedly improves standard therapy response in molecularly defined models of pancreatic cancer. Clin Cancer Res, 18, 5031-42. https://doi.org/10.1158/1078-0432.CCR-12-0453
  17. Koppe MJ, Oyen WJ, Bleichrodt RP, et al (2006). Combination therapy using the cyclooxygenase-2 inhibitor Parecoxib and radioimmunotherapy in nude mice with small peritoneal metastases of colonic origin. Cancer Immunol Immunother, 55, 47-55. https://doi.org/10.1007/s00262-005-0704-3
  18. Matsumoto S, Yasui H, Batra S, et al (2009). Simultaneous imaging of tumor oxygenation and microvascular permeability using Overhauser enhanced MRI. Proc Natl Acad Sci USA, 106, 17898-903. https://doi.org/10.1073/pnas.0908447106
  19. Pyo H, Choy H, Amorino GP, et al (2001). A selective cyclooxygenase-2 inhibitor, NS-398, enhances the effect of radiation in vitro and in vivo preferentially on the cells that express cyclooxygenase-2. Clin Cancer Res, 7, 2998-3005.
  20. Raza A, Franklin MJ, Dudek AZ (2010). Pericytes and vessel maturation during tumor angiogenesis and metastasis. Am J Hematol, 85, 593-8. https://doi.org/10.1002/ajh.21745
  21. Sauer R, Becker H, Hohenberger W (2004). Preoperative versus postoperative chemoradiotherapy for rectal cancer. N Engl J Med, 351, 1731-40. https://doi.org/10.1056/NEJMoa040694
  22. Senzaki M, Ishida S, Yada A, et al (2008). CS-706, a novel cyclooxygenase-2 selective inhibitor, prolonged the survival of tumor-bearing mice when treated alone or in combination with anti-tumor chemotherapeutic agents. Int J Cancer, 122, 1384-90.
  23. Shin YK, Park JS, Kim HS, et al (2005). Radiosensitivity enhancement by celecoxib, a cyclooxygenase (COX)-2 selective inhibitor, via COX-2-dependent cell cycle regulation on human cancer cells expressing differential COX-2 levels. Cancer Res, 65, 9501-9. https://doi.org/10.1158/0008-5472.CAN-05-0220
  24. Tang SC, Chen YC (2014). Novel therapeutic targets for pancreatic cancer. World J Gastroenterol, 20, 10825-44. https://doi.org/10.3748/wjg.v20.i31.10825
  25. Tsai JH, Makonnen S, Feldman M, et al (2005). Ionizing radiation inhibits tumor neovascularization by inducing ineffective angiogenesis. Cancer Biol Ther, 4, 1395-400. https://doi.org/10.4161/cbt.4.12.2331
  26. Yokouchi H, Kanazawa K, Ishida T, et al (2014). Cyclooxygenase-2 inhibitors for non-small-cell lung cancer: A phase II trial and literature review. Mol Clin Oncol, 2, 744-50.
  27. Valentini V, Coco C, Picciocchi A, et al (2002). Does downstaging predict improved outcome after preoperative chemoradiation for extraperitoneal locally advanced rectal cancer? A long-term analysis of 165 patients. Int J Radiat Oncol Biol Phys, 53, 664-74. https://doi.org/10.1016/S0360-3016(02)02764-5
  28. Watwe V, Javle M, Lawrence D, et al (2005). Cyclooxygenase-2 (COX-2) levels before and after chemotherapy: a study in rectal cancer. Am J Clin Oncol, 28, 560-4. https://doi.org/10.1097/01.coc.0000182476.34375.17

피인용 문헌

  1. Parecoxib inhibits glioblastoma cell proliferation, migration and invasion by upregulating miRNA-29c vol.6, pp.3, 2016, https://doi.org/10.1242/bio.021410