Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Improved Therapeutic Profiles of PLA2-Free Bee Venom Prepared by Ultrafiltration Method

  • Lee, Hyunkyoung (College of Veterinary Medicine, Gyeongsang National University) ;
  • Pyo, Min-Jung (College of Veterinary Medicine, Gyeongsang National University) ;
  • Bae, Seong Kyeong (College of Veterinary Medicine, Gyeongsang National University) ;
  • Heo, Yunwi (College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Choul Goo (Chung Jin Biotech Co., Ltd., Hanyang University Business Center) ;
  • Kang, Changkeun (College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Euikyung (College of Veterinary Medicine, Gyeongsang National University)
  • Received : 2014.10.14
  • Accepted : 2014.11.26
  • Published : 2015.03.31
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Abstract

Bee venom (BV) has long been used in traditional Eastern and Western medicine for chronic inflammation, pain and skin therapy. Human exposure to BV, however, often causes unwanted adverse effects and is even fatal in some cases. Phospholipase $A_2$ ($PLA_2$) of BV is now suspected to play a key role in these adverse effects. We investigated the potential use of $PLA_2$-free bee venom (PBV) as a replacement for BV in cosmetic products. PBV prepared by molecular weight cut-off ultrafiltration exhibits a superior profile in comparison with regular BV, by inhibiting elastase activity and suppressing the induction of nitric oxide (NO) and metalloproteinase-9 (MMP-9), while retaining the effects of cell proliferation and protection against ultraviolet B (UVB)-induced damage in human dermal fibroblast cells. PBV thus appears to be more promising than BV as a cosmetic ingredient with a reduced potential for adverse reactions in the recipient.

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References

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