Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Molecular Characterization and Expression Analysis of Adrenergic Receptor Beta 2 (ADRB2) Gene before and after Exercise in the Horse

  • Cho, Hyun-Woo (Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University) ;
  • Shin, Sangsu (Life and Industry Convergence Research Institute, College of Natural Resources and Life Sciences, Pusan National University) ;
  • Song, Ki-Duk (Genomic informatics Center, Hankyong National University) ;
  • Park, Jeong-Woong (Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University) ;
  • Choi, Jae-Young (Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University) ;
  • Lee, Hak-Kyo (Genomic informatics Center, Hankyong National University) ;
  • Cho, Byung-Wook (Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University)
  • Received : 2014.07.29
  • Accepted : 2014.09.23
  • Published : 2015.05.01
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Abstract

The adrenergic receptor beta 2 (ADRB2) plays a role in various physiological responses of the muscle to exercise, such as contraction and relaxation. Given its important role in muscle function, we investigated the structure of the horse ADRB2 gene and its expression pattern after exercise to determine if it can serve as a putative biomarker for recovery. Evolutionary analyses using synonymous and non-synonymous mutation ratios, were compared with other species (human, chimpanzee, mouse, rat, cow, pig, chicken, dog, and cat), and revealed the occurrence of positive selection in the horse ADRB2 gene. In addition, expression analyses by quantitative polymerase chain reaction exhibited ubiquitous distribution of horse ADRB2 in various tissues including lung, skeletal muscle, kidney, thyroid, appendix, colon, spinal cord and heart, with the highest expression observed in the lung. The expression of ADRB2 in skeletal muscle was significantly up-regulated about four folds 30 minutes post-exercise compared to pre-exercise. The expression level of ADRB2 in leukocytes, which could be collected with convenience compared with other tissues in horse, increased until 60 min after exercise but decreased afterward until 120 min, suggesting the ADRB2 expression levels in leukocytes could be a useful biomarker to check the early recovery status of horse after exercise. In conclusion, we identified horse ADRB2 gene and analyzed expression profiles in various tissues. Additionally, analysis of ADBR2 gene expression in leukocytes could be a useful biomarker useful for evaluation of early recovery status after exercise in racing horses.

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References

  1. Bustamante, C. D., A. Fledel-Alon, S. Williamson, R. Nielsen, M. T. Hubisz, S. Glanowski, D. M. Tanenbaum, T. J. White, J. J. Sninsky, R. D. Hernandez, D. Civello, M. D. Adams, M. Cargill, and A. G. Clark. 2005. Natural selection on proteincoding genes in the human genome. Nature 437:1153-1157. https://doi.org/10.1038/nature04240
  2. Hegab, A. E., T. Sakamoto, W. Saitoh, H. H. Massoud, H. M. Massoud, K. M. Hassanein, and K. Sekizawa. 2004. Polymorphisms of IL4, IL13, and ADRB2 genes in COPD. Chest 126:1832-1839. https://doi.org/10.1378/chest.126.6.1832
  3. Ho, L. I., H. J. Harn, C. J. Chen, and N. M. Tsai. 2001. Polymorphism of the beta(2)-adrenoceptor in COPD in Chinese subjects. Chest 120:1493-1499. https://doi.org/10.1378/chest.120.5.1493
  4. Joos, L., T. D. Weir, J. E. Connett, N. R. Anthonisen, R. Woods, P. D. Pare, and A. J. Sandford. 2003. Polymorphisms in the beta2 adrenergic receptor and bronchodilator response, bronchial hyperresponsiveness, and rate of decline in lung function in smokers. Thorax 58:703-707. https://doi.org/10.1136/thorax.58.8.703
  5. Livak, K. J. and T. D. Schmittgen. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(- Delta Delta C(T)) method. Methods 25:402-408. https://doi.org/10.1006/meth.2001.1262
  6. MacNeil, L. G., S. Melov, A. E. Hubbard, S. K. Baker, and M. A. Tarnopolsky. 2010. Eccentric exercise activates novel transcriptional regulation of hypertrophic signaling pathways not affected by hormone changes. PLoS ONE 5(5):e10695. https://doi.org/10.1371/journal.pone.0010695
  7. McGraw, D. W. and S. B. Liggett. 2005. Molecular mechanisms of beta2-adrenergic receptor function and regulation. Proc. Am. Thorac. Soc. 2:292-296. https://doi.org/10.1513/pats.200504-027SR
  8. Park, K. D., J. Park, J. Ko, B. C. Kim, H. S. Kim, K. Ahn, K. T. Do, H. Choi, H. M. Kim, S. Song, S. Lee, S. Jho, H. S. Kong, Y. M. Yang, B. H. Jhun, C. Kim, T. H. Kim, S. Hwang, J. Bhak, H. K. Lee, and B. W. Cho. 2012. Whole transcriptome analyses of six thoroughbred horses before and after exercise using RNA-Seq. BMC Genomics 13:473. https://doi.org/10.1186/1471-2164-13-473
  9. Rang, H. P., M. M. Dale, J. M. Ritter, and P. K. Moore. 2003. Pharmacology. 5th ed. Churchill Livingstone, Edinburgh, Scotland.
  10. Saitou, N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406-425.
  11. Tamura, K., D. Peterson, N. Peterson, G. Stecher, M. Nei, and S. Kumar. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28:2731-2739. https://doi.org/10.1093/molbev/msr121
  12. Wolfarth, B., T. Rankinen, S. Muhlbauer, J. Scherr, M. R. Boulay, L. Perusse, R. Rauramaa, and C. Bouchard. 2007. Association between a beta2-adrenergic receptor polymorphism and elite endurance performance. Metabolism. 56:1649-1651. https://doi.org/10.1016/j.metabol.2007.07.006
  13. Zieker, D., J. Zieker, J. Dietzsch, M. Burnet, H. Northoff, and E. Fehrenbach. 2005. CDNA-microarray analysis as a research tool for expression profiling in human peripheral blood following exercise. Exerc. Immunol. Rev. 11:86-96.

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