The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Nicotine in High Concentration Causes Contraction of Isolated Strips of Rabbit Corpus Cavernosum

  • Nguyen, Hoai Bac (Advanced Urogenital Disease Research Center, Research Institute for Translational System Biomics, Department of Urology, Chung-Ang University Hospital) ;
  • Lee, Shin Young (Department of Urology, Seoul Medical Center) ;
  • Park, Soo Hyun (Department of Physiology, College of Medicine, Chung-Ang University) ;
  • Han, Jun Hyun (Department of Urology, Hallym University Dongtan Sacred Heart Hospital) ;
  • Lee, Moo Yeol (Department of Physiology, College of Medicine, Chung-Ang University) ;
  • Myung, Soon Chul (Advanced Urogenital Disease Research Center, Research Institute for Translational System Biomics, Department of Urology, Chung-Ang University Hospital)
  • Received : 2015.01.05
  • Accepted : 2015.04.08
  • Published : 2015.05.01
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Abstract

It is well known that cigarette smoke can cause erectile dysfunction by affecting the penile vascular system. However, the exact effects of nicotine on the corpus cavernosum remains poorly understood. Nicotine has been reported to cause relaxation of the corpus cavernosum; it has also been reported to cause both contraction and relaxation. Therefore, high concentrations of nicotine were studied in strips from the rabbit corpus cavernosum to better understand its effects. The proximal penile corpus cavernosal strips from male rabbits weighing approximately 4 kg were used in organ bath studies. Nicotine in high concentrations ($10^{-5}{\sim}10^{-4}M$) produced dose-dependent contractions of the corpus cavernosal strips. The incubation with $10^{-5}M$ hexamethonium (nicotinic receptor antagonist) significantly inhibited the magnitude of the nicotine associated contractions. The nicotine-induced contractions were not only significantly inhibited by pretreatment with $10^{-5}M$ indomethacin (nonspecific cyclooxygenase inhibitor) and with $10^{-6}M$ NS-398 (selective cyclooxygenase inhibitor), but also with $10^{-6}M$ Y-27632 (Rho kinase inhibitor). Ozagrel (thromboxane $A_2$ synthase inhibitor) and SQ-29548 (highly selective TP receptor antagonist) pretreatments significantly reduced the nicotine-induced contractile amplitude of the strips. High concentrations of nicotine caused contraction of isolated rabbit corpus cavernosal strips. This contraction appeared to be mediated by activation of nicotinic receptors. Rho-kinase and cyclooxygenase pathways, especially cyclooxygenase-2 and thromboxane $A_2$, might play a pivotal role in the mechanism associated with nicotine-induced contraction of the rabbit corpus cavernosum.

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References

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