Asian Pacific Journal of Cancer Prevention

  • 제16권16호
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  • Pages.7155-7159
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  • 2015
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  • 1513-7368(pISSN)
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  • 2476-762X(eISSN)
아시아태평양암예방학회 (Asian Pacific Journal of Cancer Prevention)
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Cytotoxicity of Cratoxylum Formosum Subsp. Pruniflorum Gogel Extracts in Oral Cancer Cell Lines

  • 발행 : 2015.11.04
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초록

Background: Oral cancer is a health problem in Thailand. Cratoxylum formosum subsp. pruniflorum Gogel (Teawdang), normally consumed in northeast Thailand, has proven cytotoxic to cervical cancer cell lines including HeLa, SiHa and C-33A. Recently, Asian oral cancer cell lines, ORL-48 and ORL-136, were established. Therefore, we aimed to study cytotoxicity of Teawdang in these. Total phenolic (TPC) and flavonoid content (TFC), and antioxidant activity of Teawdang were also determined. Materials and Methods: Teawdang was purchased from Khon Kaen market during June-October 2013. Hexane (CHE), ethyl acetate (CEE) and methanol (CME) extracts of its edible part were analyzed for TPC by the folin-ciocalteau method and for TFC by an aluminium colorimetric method. Antioxidant activity and cytotoxicity in normal Vero cells and oral cancer cells were investigated. Cell viability was assessed using 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assays. Results: CME and CEE had higher TPC and TFC and antioxidant activity than CHE. Both CME and CEE, at $200{\mu}g$ dry wt/mL, were cytotoxic to the studied oral cancer cell lines. However, CME was cytotoxic to Vero cells whereas CEE was not. Compared to Vero cells, CEE significantly inhibited ORL-48 and ORL-136 growth (p=0.03 and p=0.02, respectively). Conclusions: CEE exhibited cytotoxic effects on the studied oral cancer cell lines but not normal Vero cells. The bioactive compounds in CEE should be further purified and elucidated for their mechanisms of action for development as anticancer agents.

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참고문헌

  1. Benzie IF, Strain JJ (1996). The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem, 239, 70-6. https://doi.org/10.1006/abio.1996.0292
  2. Chen M, Rao Y, Zheng Y, et al (2014). Association between soy isoflavone intake and breast cancer risk for pre- and postmenopausal women: a meta-analysis of epidemiological studies. PLoS One, 9, 89288. https://doi.org/10.1371/journal.pone.0089288
  3. Clarke G, Ting K, Wiart C, et al (2013). High correlation of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric reducing activity potential and total phenolics content indicates redundancy in use of all three assays to screen for antioxidant activity of extracts of plants from the malaysian rainforest. Antioxidants, 2, 1-10. https://doi.org/10.3390/antiox2010001
  4. Daduang J, Vichitphan S, Daduang S, et al (2011). High phenolics and antioxidants of some tropical vegetables related to antibacterial and anticancer activities. Afr J Pharm Pharmacol, 5, 608-15. https://doi.org/10.5897/AJPP10.243
  5. Di Domenico F, Foppoli C, Coccia R, et al (2012). Antioxidants in cervical cancer: chemopreventive and chemotherapeutic effects of polyphenols. Biochim Biophys Acta, 1822, 737-47. https://doi.org/10.1016/j.bbadis.2011.10.005
  6. Dzagnidze A, Katsarava Z, Makhalova J, et al (2007). Repair capacity for platinum-DNA adducts determines the severity of cisplatin-induced peripheral neuropathy. J Neurosci, 27, 9451-7. https://doi.org/10.1523/JNEUROSCI.0523-07.2007
  7. Ferlay J, Soerjomataram I, Ervik M, et al (2013). GLOBOCAN 2012 v1.0, Cancer Incidence and Mortality Worldwide: IARC Cancer Base No. 11 (Internet). Lyon, France: International Agency for Research on Cancer; Available from: http://globocan.iarc.fr.
  8. Gillison ML, D’Souza G, Westra W, et al (2008). Distinct risk factor profiles for human papillomavirus type 16-positive and human papillomavirus type 16-negative head and neck cancers. J Natl Cancer Inst, 100, 407-20. https://doi.org/10.1093/jnci/djn025
  9. Hamid S, Lim KP, Zain RB, et al (2007). Establishment and characterization of Asian oral cancer cell lines as in vitro models to study a disease prevalent in Asia. Int J Mol Med, 19, 453-60.
  10. Heck JE, Berthiller J, Vaccarella S, et al (2010). Sexual behaviours and the risk of head and neck cancers: a pooled analysis in the International Head and Neck Cancer Epidemiology (INHANCE) consortium. Int J Epidemiol, 39, 166-81. https://doi.org/10.1093/ije/dyp350
  11. Issara-Amphorn J, T-Thienprasert NP (2014). Preliminary in vitro pro-apoptotic effects of Cratoxylum formosum crude leaf extracts. Int J Appl Res Nat Prod, 7, 26-30.
  12. Jiang Y, Kusama K, Satoh K, et al (2000). Induction of cytotoxicity by chlorogenic acid in human oral tumor cell lines. Phytomedicine, 7, 483-91. https://doi.org/10.1016/S0944-7113(00)80034-3
  13. Kang HJ, Jang YJ (2012). Selective apoptotic effect of Zelkova serrata twig extract on mouth epidermoid carcinoma through p53 activation. Int J Oral Sci, 4, 78-84. https://doi.org/10.1038/ijos.2012.14
  14. Khuhaprema T, Srivatanakul P, Sriplung H, et al (2010). Cancer in Thailand (2001-2003). National Cancer Institute Department of Med Services Ministry of Public Hlth, 5, 11-5.
  15. Kim SH, Kim HJ, Lee MH, et al (2011). Resveratrol induces apoptosis of KB human oral cancer cells. J Korean Soc Appl Bl, 54, 966-71. https://doi.org/10.1007/BF03253187
  16. Kukongviriyapan U, Luangaram S, Leekhaosoong K, et al (2007). Antioxidant and vascular protective activities of Cratoxylum formosum, Syzygium gratum and Limnophila aromatica. Biol Pharm Bull, 30, 661-6. https://doi.org/10.1248/bpb.30.661
  17. Kuo CL, Lai KC, Ma YS, et al (2014). Gallic acid inhibits migration and invasion of SCC-4 human oral cancer cells through actions of NF-${\kappa}B$, Ras and matrix metalloproteinase-2 and -9. Oncol Rep, 32, 355-61.
  18. Lai WW, Hsu SC, Chueh FS, et al (2013). Quercetin inhibits migration and invasion of SAS human oral cancer cells through inhibition of NF-${\kappa}B$ and matrix metalloproteinase-2/-9 signaling pathways. Anticancer Res, 33, 1941-50.
  19. Mahavorasirikul W, Viyanant V, Chaijaroenkul W, et al (2010). Cytotoxic activity of Thai medicinal plants against human cholangiocarcinoma, laryngeal and hepatocarcinoma cells in vitro. BMC Complement Altern Med, 10, 55. https://doi.org/10.1186/1472-6882-10-55
  20. Manosroi A, Akazawa H, Pattamapun K, et al (2015). Potent anti-proliferative effects against oral and cervical cancers of Thai medicinal plants selected from the Thai/Lanna medicinal plant recipe database “MANOSROI III”. Pharm Biol, 53, 1075-81 https://doi.org/10.3109/13880209.2014.959613
  21. Maisuthisakul P, Pongsawatmanit R, Gordon MH (2006). Antioxidant properties of Teaw ( Cratoxylum formosum Dyer) extract in soybean oil and emulsions. J Agric Food Chem, 54, 2719-25. https://doi.org/10.1021/jf052396+
  22. Maisuthisakul P, Pongsawatmanit R, Gordon MH (2007). Characterization of the phytochemicals and antioxidant properties of extracts from Teaw (Cratoxylum formosum Dyer). Food Chem, 100, 1620-9. https://doi.org/10.1016/j.foodchem.2005.12.044
  23. Majid MZ,Zaini ZM,Razak FA (2014). Apoptosis-Inducing effect of three medicinal plants on oral cancer cells KB and ORL-48. Sci World J, 2014, 125353
  24. Ng IO, Lam KY, Ng M, et al (1998). Expression of P-glycoprotein, a multidrug-resistance gene product, is induced by radiotherapy in patients with oral squamous cell carcinoma. Cancer, 83, 851-7. https://doi.org/10.1002/(SICI)1097-0142(19980901)83:5<851::AID-CNCR8>3.0.CO;2-L
  25. Nonpunya A, Weerapreeyakul N, Barusrux S (2014). Cratoxylum formosum (Jack) Dyer ssp. pruniflorum (Kurz) Gogel. (Hong ya mu) extract induces apoptosis in human hepatocellular carcinoma HepG2 cells through caspase-dependent pathways. Chin Med, 9, 12. https://doi.org/10.1186/1749-8546-9-12
  26. Palasap A, Limpaiboon T, Boonsiri P, et al (2014). Cytotoxic effects of phytophenolics from Caesalpinia mimosoides Lamk on cervical carcinoma cell lines through an apoptotic pathway. Asian Pac J Cancer Prev, 15, 449-54. https://doi.org/10.7314/APJCP.2014.15.1.449
  27. Patel A, Patel A, Patel A, et al (2010). Determination of polyphenols and free radical scavenging activity of Tephrosia purpurea linn leaves (Leguminosae). Pharmacognosy Res, 2, 152-8. https://doi.org/10.4103/0974-8490.65509
  28. Patel BP, Rawal UM, Rawal RM, et al (2008). Tobacco, antioxidant enzymes, oxidative stress, and genetic susceptibility in oral cancer. Am J Clin Oncol, 31, 454-9. https://doi.org/10.1097/COC.0b013e31816a61da
  29. Pathak J, Swain N, Patel S, et al (2014). Histopathological variants of oral squamous cell carcinoma-institutional case reports. J Oral Maxillofac Pathol, 18, 143-5.
  30. Perez-Sayans M, Somoza-Martin JM, Barros-Angueira F, et al (2010). Multidrug resistance in oral squamous cell carcinoma: The role of vacuolar ATPases. Cancer Lett, 295, 135-43. https://doi.org/10.1016/j.canlet.2010.03.019
  31. Promraksa B, Daduang J, Khampitak T, et al (2015). Anticancer potential of Cratoxylum formosum subsp. pruniflorum (Kurz.) Gogel. extracts against cervical cancer cell lines. Asian Pac J Cancer Prev. (In press)
  32. Ramkissoon JS, Mahomoodally MF, Ahmed N, et al (2013). Antioxidant and anti-glycation activities correlates with phenolic composition of tropical medicinal herbs. Asian Pac J Trop Med, 6, 561-9. https://doi.org/10.1016/S1995-7645(13)60097-8
  33. Sebai H, Souli A, Chehimi L, et al (2013). In vitro and in vivo antioxidant properties of Tunisian carob (Ceratonia siliqua L.). J Med Plants Res, 7, 85-90.
  34. Sturgis EM, Wei Q, Spitz MR (2004). Descriptive epidemiology and risk factors for head and neck cancer. Semin Oncol, 31, 726-33. https://doi.org/10.1053/j.seminoncol.2004.09.013
  35. Surh YJ (2003). Cancer chemoprevention with dietary phytochemicals. Nat Rev Cancer, 3, 768-80. https://doi.org/10.1038/nrc1189
  36. Vatanasapt P, Suwanrungruang K, Kamsa-Ard S, et al (2011). Epidemiology of oral and pharyngeal cancers in Khon Kaen, Thailand: a high incidence in females. Asian Pac J Cancer Prev, 12, 2505-8.
  37. Waiyaput W, Payungporn S, Issara-Amphorn J, et al (2012). Inhibitory effects of crude extracts from some edible Thai plants against replication of hepatitis B virus and human liver cancer cells. BMC Complement Altern Med, 12, 246 https://doi.org/10.1186/1472-6882-12-246
  38. Wang X, Ouyang Y, Liu J, et al (2014). Fruit and vegetable consumption and mortality from all causes, cardiovascular disease, and cancer: systematic review and dose-response meta-analysis of prospective cohort studies. BMJ, 349, 4490. https://doi.org/10.1136/bmj.g4490
  39. Woraratphoka J, Intarapichet K, Indrapichate K (2012). Antioxidant activity and cytotoxicity of six selected, regional, Thai vegetables. Am-Euras J Toxicol Sci, 4, 108-17.
  40. Xiong J, Liu XH, Bui VB, et al (2014). Phenolic constituents from the leaves of Cratoxylum formosum ssp. pruniflorum. Fitoterapia, 94, 114-9. https://doi.org/10.1016/j.fitote.2014.02.002
  41. Zanaruddin SN, Yee PS, Hor SY, et al (2013). Common oncogenic mutations are infrequent in oral squamous cell carcinoma of Asian origin. PLoS One, 8, 80229. https://doi.org/10.1371/journal.pone.0080229

피인용 문헌

  1. Cytotoxic and antimigratory effects of Cratoxy formosum extract against HepG2 liver cancer cells vol.6, pp.4, 2017, https://doi.org/10.3892/br.2017.871