The Korean Journal of Physiology and Pharmacology

  • 제20권2호
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  • Pages.147-152
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  • 2016
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  • 1226-4512(pISSN)
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  • 2093-3827(eISSN)
대한약리학회 (The Korean Society of Pharmacology)
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Curcumin protects against the intestinal ischemia-reperfusion injury: involvement of the tight junction protein ZO-1 and TNF-α related mechanism

  • Tian, Shuying (Department of Gastroenterology, Cangzhou Central Hospital) ;
  • Guo, Ruixue (Department of Gastroenterology, Cangzhou Central Hospital) ;
  • Wei, Sichen (Department of Gastroenterology, Cangzhou Central Hospital) ;
  • Kong, Yu (Department of Gastroenterology, Cangzhou Central Hospital) ;
  • Wei, Xinliang (Department of Gastroenterology, Cangzhou Central Hospital) ;
  • Wang, Weiwei (Department of Gastroenterology, Cangzhou Central Hospital) ;
  • Shi, Xiaomeng (Department of Gastroenterology, Cangzhou Central Hospital) ;
  • Jiang, Hongyu (Department of Gastroenterology, Cangzhou Central Hospital)
  • 투고 : 2015.02.16
  • 심사 : 2015.09.24
  • 발행 : 2016.03.01
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초록

Present study aimed to investigate the effect of curcumin-pretreatment on intestinal I/R injury and on intestinal mucosa barrier. Thirty Wistar rats were randomly divided into: sham, I/R, and curcumin groups (n=10). Animals in curcumin group were pretreated with curcumin by gastric gavage (200 mg/kg) for 2 days before I/R. Small intestine tissues were prepared for Haematoxylin & Eosin (H&E) staining. Serum diamine oxidase (DAO) and tumor necrosis factor (TNF)-${\alpha}$ levels were measured. Expression of intestinal TNF-${\alpha}$ and tight junction protein (ZO-1) proteins was detected by Western blot and/or immunohistochemistry. Serum DAO level and serum and intestinal TNF-${\alpha}$ leves were significantly increased after I/R, and the values were markedly reduced by curcumin pretreatment although still higher than that of sham group (p<0.05 or p<0.001). H&E staining showed the significant injury to intestinal mucosa following I/R, and curcumin pretreatment significantly improved the histological structure of intestinal mucosa. I/R insult also induced significantly down-regulated expression of ZO-1, and the effect was dramatically attenuated by curcumin-pretreatment. Curcumin may protect the intestine from I/R injury through restoration of the epithelial structure, promotion of the recovery of intestinal permeability, as well as enhancement of ZO-1 protein expression, and this effect may be partly attributed to the TNF-${\alpha}$ related pathway.

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