Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Comparison of in Vitro Cytotoxicity and Apoptogenic Activity of Magnesium Chloride and Cisplatin as Conventional Chemotherapeutic Agents in the MCF-7 Cell Line

  • Mirmalek, Seyed Abbas (Department of Surgery, Islamic Azad University, Tehran Medical Sciences Branch) ;
  • Jangholi, Ehsan (Young Researchers and Elite Club, Islamic Azad University, Tehran Medical Sciences Branch) ;
  • Jafari, Mohammad (Department of Surgery, Faculty of Medical Sciences, Arak University of Medical Sciences) ;
  • Yadollah-Damavandi, Soheila (Young Researchers and Elite Club, Islamic Azad University, Tehran Medical Sciences Branch) ;
  • Javidi, Mohammad Amin (Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University) ;
  • Parsa, Yekta (Young Researchers and Elite Club, Islamic Azad University, Tehran Medical Sciences Branch) ;
  • Parsa, Tina (Young Researchers and Elite Club, Islamic Azad University, Tehran Medical Sciences Branch) ;
  • Salimi-Tabatabaee, Seyed Alireza (Young Researchers and Elite Club, Islamic Azad University, Tehran Medical Sciences Branch) ;
  • Kolagar, Hossein Ghasemzadeh (Students Research Committee, School of Public Health, Shahroud University of Medical Sciences) ;
  • Jalil, Saeed Khazaei (School of Nursing and Midwifery, Shahroud University of Medical Sciences) ;
  • Alizadeh-Navaei, Reza (Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences)
  • Published : 2016.06.01
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Abstract

Breast cancer is the most common malignancy and also the second leading cause of cancer death among women and also in women that have a high mortality. Previous studies showed that magnesium (Mg) has cytotoxic effects on malignant cell lines. However, the anti-cancer effects of Mg on MCF-7 breast cancer cells are uncertain. This study was aimed at the comparison of the cytotoxic effect of Mg salt (MgCl2) and cisplatin on MCF-7 cells and fibroblasts (as normal cells). After treatment with various concentrations of MgCl2, and cisplatin as a positive control for 24 and 48 hours (h), cytotoxicity activity was measured by MTT assay. In addition, apoptosis was determined by annexin V/propidium iide assay. Both cisplatin and the MgCl2 exhibited dose-dependent cytotoxic effects in the MCF-7 cell line, although the LD50 of the Mg was significantly higher when compared to cispaltin ($40{\mu}g/ml$ vs. $20{\mu}g/ml$). Regarding annexin V/propidium results, treatment of MCF-7 cells with LD50 concentrations of cisplatin and Mg showed 59% and 44% apoptosis at 24h, respectively. Finally, the results indicated that Mg has cytotoxic effects on MCF-7 cells, but less than cisplatin as a conventional chemotherapeutic agent. However, regarding the side effects of chemotherapy drugs, it seems that Mg can be considered as a supplement for the treatment of breast cancer.

Keywords

References

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