Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Plasma Peptidome as a Source of Biomarkers for Diagnosis of Cholangiocarcinoma

  • Kotawong, Kanawut (Graduate Program in Bioclinical Sciences, Chulabhorn International College of Medicine) ;
  • Thitapakorn, Veerachai (Graduate Program in Bioclinical Sciences, Chulabhorn International College of Medicine) ;
  • Roytrakul, Sittiruk (Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Thammasat University) ;
  • Phaonakrop, Narumon (Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Thammasat University) ;
  • Viyanant, Vithoon (Graduate Program in Bioclinical Sciences, Chulabhorn International College of Medicine) ;
  • Na-Bangchang, Kesara (Graduate Program in Bioclinical Sciences, Chulabhorn International College of Medicine)
  • Published : 2016.04.11
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Abstract

Cholangiocarcinoma (CCA) is the bile duct cancer which constitutes one of the important public health problems in Thailand with high mortality rate, especially in the Opisthorchis viverrini (a parasite risk factor for CCA) endemic area of the northeastern region of the country. This study aimed to identify potential biomarkers from the plasma peptidome by CCA patients. Peptides were isolated using 10 kDa cut-off filter column and the flow-through was then used as a peptidome for LC-MS/MS analysis. A total of 209 peptides were obtained. Among these, 15 peptides were concerned with signaling pathways and 12 related to metabolic, regulatory, and biosynthesis of secondary metabolite pathways. Five exclusive peptides were identified as potential biomarkers, i.e. ETS domain-containing transcription factor ERF (P50548), KIAA0220 (Q92617), phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform isoform 1 (P42338), LP2209 (Q6XYC0), and casein kinase II subunit alpha (P19784). Three of these biomarkers are signaling related molecules. A combination of these biomarkers for CCA diagnosis is proposed.

Keywords

References

  1. Dbouk HA, Pang H, Fiser A, et al (2010). A biochemical mechanism for the oncogenic potential of the p110beta catalytic subunit of phosphoinositide 3-kinase. Proc Natl Acad Sci USA, 107, 19897-902. https://doi.org/10.1073/pnas.1008739107
  2. Fan NJ, Gao CF, Zhao G, et al (2012). Serum peptidome patterns of breast cancer based on magnetic bead separation and mass spectrometry analysis. Diagn Pathol, 7, 45. https://doi.org/10.1186/1746-1596-7-45
  3. Fukuoka K, Kuribayashi K, Yamada S, et al (2013). Combined serum mesothelin and carcinoembryonic antigen measurement in the diagnosis of malignant mesothelioma. Mol Clin Oncol, 1, 942-8. https://doi.org/10.3892/mco.2013.175
  4. Hamacher R, Saur D, Fritsch R, et al (2007). Casein kinase II inhibition induces apoptosis in pancreatic cancer cells. Oncol Rep, 18, 695-701.
  5. Jia J, Wang W, Meng W, et al (2014). Development of a multiplex autoantibody test for detection of lung cancer. PLoS One, 9, 95444. https://doi.org/10.1371/journal.pone.0095444
  6. Jia S, Liu Z, Zhang S, et al (2008). Essential roles of PI(3) K-p110beta in cell growth, metabolism and tumorigenesis. Nature, 454, 776-9. https://doi.org/10.1038/nature07091
  7. Kadoglou NP, Fotiadis G, Lambadiari V, et al (2014). Serum levels of novel adipokines in patients with acute ischemic stroke: Potential contribution to diagnosis and prognosis. Peptides.
  8. Lowry OH, Rosebrough NJ, Farr AL, et al (1951). Protein measurement with the Folin phenol reagent. J Biol Chem, 193, 265-75.
  9. Maldaner FR, Aragao FJ, dos Santos FB, et al (2013). Dengue virus tetra-epitope peptide expressed in lettuce chloroplasts for potential use in dengue diagnosis. Appl Microbiol Biotechnol, 97, 5721-9. https://doi.org/10.1007/s00253-013-4918-6
  10. Mi H, Muruganujan A, Casagrande JT, et al (2013). Large-scale gene function analysis with the PANTHER classification system. Nat Protoc, 8, 1551-66. https://doi.org/10.1038/nprot.2013.092
  11. Pinlaor S, Onsurathum S, Boonmars T, et al (2013). Distribution and abundance of Opisthorchis viverrini metacercariae in cyprinid fish in Northeastern Thailand. Korean J Parasitol, 51, 703-10. https://doi.org/10.3347/kjp.2013.51.6.703
  12. Seldin DC, Landesman-Bollag E, Farago M, et al (2005). CK2 as a positive regulator of Wnt signalling and tumourigenesis. Mol Cell Biochem, 274, 63-7. https://doi.org/10.1007/s11010-005-3078-0
  13. Sgouras DN, Athanasiou MA, Beal GJ, Jr., et al (1995). ERF: an ETS domain protein with strong transcriptional repressor activity, can suppress ets-associated tumorigenesis and is regulated by phosphorylation during cell cycle and mitogenic stimulation. EMBO J, 14, 4781-93.
  14. Song DH, Sussman DJ, Seldin DC (2000). Endogenous protein kinase CK2 participates in Wnt signaling in mammary epithelial cells. J Biol Chem, 275, 23790-7. https://doi.org/10.1074/jbc.M909107199
  15. Sripa B, Pairojkul C (2008). Cholangiocarcinoma: lessons from Thailand. Curr Opin Gastroenterol, 24, 349-56. https://doi.org/10.1097/MOG.0b013e3282fbf9b3
  16. Terracciano R, Preiano M, Palladino GP, et al (2011). Peptidome profiling of induced sputum by mesoporous silica beads and MALDI-TOF MS for non-invasive biomarker discovery of chronic inflammatory lung diseases. Proteomics, 11, 3402-14. https://doi.org/10.1002/pmic.201000828
  17. Wee S, Wiederschain D, Maira SM, et al (2008). PTEN-deficient cancers depend on PIK3CB. Proc Natl Acad Sci U S A, 105, 13057-62. https://doi.org/10.1073/pnas.0802655105
  18. Xiao D, Meng FL, He LH, et al (2011). Analysis of the urinary peptidome associated with Helicobacter pylori infection. World J Gastroenterol, 17, 618-24. https://doi.org/10.3748/wjg.v17.i5.618
  19. Xu Y, Zhang L, Sun SK, et al (2014). CC chemokine ligand 18 and IGF-binding protein 6 as potential serum biomarkers for prostate cancer. Tohoku J Exp Med, 233, 25-31. https://doi.org/10.1620/tjem.233.25
  20. Yamada T, Letunic I, Okuda S, et al (2011). iPath2.0: interactive pathway explorer. Nucleic Acids Res, 39, 412-5. https://doi.org/10.1093/nar/gkr313
  21. Yang J, Song YC, Song TS, et al (2012). Identification of novel low molecular weight serum peptidome biomarkers for non-small cell lung cancer (NSCLC). J Clin Lab Anal, 26, 148-54. https://doi.org/10.1002/jcla.21502
  22. Yin QS, Shi B, Dong L, et al (2014). Comparative study of galectin-3 and B-type natriuretic peptide as biomarkers for the diagnosis of heart failure. J Geriatr Cardiol, 11, 79-82.
  23. Zaldumbide A, Carlotti F, Pognonec P, et al (2002). The role of the Ets2 transcription factor in the proliferation, maturation, and survival of mouse thymocytes. J Immunol, 169, 4873-81. https://doi.org/10.4049/jimmunol.169.9.4873

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