The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Voluntary stand-up physical activity enhances endurance exercise capacity in rats

  • Seo, Dae Yun (National Research Laboratory for Mitochondrial Signaling, Department of Physiology, Department of Health Sciences and Technology, BK21 Project Team, College of Medicine, Cardiovascular and Metabolic Disease Center, Inje University) ;
  • Lee, Sung Ryul (National Research Laboratory for Mitochondrial Signaling, Department of Physiology, Department of Health Sciences and Technology, BK21 Project Team, College of Medicine, Cardiovascular and Metabolic Disease Center, Inje University) ;
  • Kwak, Hyo-Bum (Department of Kinesiology, Inha University) ;
  • Seo, Kyo Won (National Research Laboratory for Mitochondrial Signaling, Department of Physiology, Department of Health Sciences and Technology, BK21 Project Team, College of Medicine, Cardiovascular and Metabolic Disease Center, Inje University) ;
  • McGregor, Robin A (National Research Laboratory for Mitochondrial Signaling, Department of Physiology, Department of Health Sciences and Technology, BK21 Project Team, College of Medicine, Cardiovascular and Metabolic Disease Center, Inje University) ;
  • Yeo, Ji Young (National Research Laboratory for Mitochondrial Signaling, Department of Physiology, Department of Health Sciences and Technology, BK21 Project Team, College of Medicine, Cardiovascular and Metabolic Disease Center, Inje University) ;
  • Ko, Tae Hee (National Research Laboratory for Mitochondrial Signaling, Department of Physiology, Department of Health Sciences and Technology, BK21 Project Team, College of Medicine, Cardiovascular and Metabolic Disease Center, Inje University) ;
  • Bolorerdene, Saranhuu (National Research Laboratory for Mitochondrial Signaling, Department of Physiology, Department of Health Sciences and Technology, BK21 Project Team, College of Medicine, Cardiovascular and Metabolic Disease Center, Inje University) ;
  • Kim, Nari (National Research Laboratory for Mitochondrial Signaling, Department of Physiology, Department of Health Sciences and Technology, BK21 Project Team, College of Medicine, Cardiovascular and Metabolic Disease Center, Inje University) ;
  • Ko, Kyung Soo (National Research Laboratory for Mitochondrial Signaling, Department of Physiology, Department of Health Sciences and Technology, BK21 Project Team, College of Medicine, Cardiovascular and Metabolic Disease Center, Inje University) ;
  • Rhee, Byoung Doo (National Research Laboratory for Mitochondrial Signaling, Department of Physiology, Department of Health Sciences and Technology, BK21 Project Team, College of Medicine, Cardiovascular and Metabolic Disease Center, Inje University) ;
  • Han, Jin (National Research Laboratory for Mitochondrial Signaling, Department of Physiology, Department of Health Sciences and Technology, BK21 Project Team, College of Medicine, Cardiovascular and Metabolic Disease Center, Inje University)
  • Received : 2015.12.11
  • Accepted : 2016.03.02
  • Published : 2016.05.01
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Abstract

Involuntary physical activity induced by the avoidance of electrical shock leads to improved endurance exercise capacity in animals. However, it remains unknown whether voluntary stand-up physical activity (SPA) without forced simulating factors improves endurance exercise capacity in animals. We examined the effects of SPA on body weight, cardiac function, and endurance exercise capacity for 12 weeks. Twelve male Sprague-Dawley rats (aged 8 weeks, n=6 per group) were randomly assigned to a control group (CON) or a voluntary SPA group. The rats were induced to perform voluntary SPA (lifting a load equal to their body weight), while the food height (18.0 cm) in cages was increased progressively by 3.5 every 4 weeks until it reached 28.5 cm for 12 weeks. The SPA group showed a lower body weight compared to the CON group, but voluntary SPA did not affect the skeletal muscle and heart weights, food intake, and echocardiography results. Although the SPA group showed higher grip strength, running time, and distance compared to the CON group, the level of irisin, corticosterone, genetic expression of mitochondrial biogenesis, and nuclei numbers were not affected. These findings show that voluntary SPA without any forced stimuli in rats can effectively reduce body weight and enhance endurance exercise capacity, suggesting that it may be an important alternative strategy to enhance endurance exercise capacity.

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References

  1. Bang HS, Seo DY, Chung YM, Oh KM, Park JJ, Arturo F, Jeong SH, Kim N, Han J. Ursolic Acid-induced elevation of serum irisin augments muscle strength during resistance training in men. Korean J Physiol Pharmacol. 2014;18:441-446. https://doi.org/10.4196/kjpp.2014.18.5.441
  2. Kim JS, Kim MG, Kang SW, Kim BR, Beak MY, Park YM, Shin MS. Association of obesity, hypertension and their impact on cardiac function and exercise capacity. Korean Circ J. 2016;46:e51.
  3. Jung SR, Kim KJ. Exercise-induced PGC-1 transcriptional factors in skeletal muscle. Integrative Medicine Research. 2014;3:155-160. https://doi.org/10.1016/j.imr.2014.09.004
  4. Constable SH, Favier RJ, McLane JA, Fell RD, Chen M, Holloszy JO. Energy metabolism in contracting rat skeletal muscle: adaptation to exercise training. Am J Physiol. 1987;253:C316-322. https://doi.org/10.1152/ajpcell.1987.253.2.C316
  5. Takada S, Kinugawa S, Matsushima S, Takemoto D, Furihata T, Mizushima W, Fukushima A, Yokota T, Ono Y, Shibata H, Okita K, Tsutsui H. Sesamin prevents decline in exercise capacity and impairment of skeletal muscle mitochondrial function in mice with high-fat diet-induced diabetes. Exp Physiol. 2015;11:1319-1330.
  6. Edelmann F, Gelbrich G, Dungen HD, Frohling S, Wachter R, Stahrenberg R, Binder L, Topper A, Lashki DJ, Schwarz S, Herrmann-Lingen C, Loffler M, Hasenfuss G, Halle M, Pieske B. Exercise training improves exercise capacity and diastolic function in patients with heart failure with preserved ejection fraction: results of the Ex-DHF (Exercise training in Diastolic Heart Failure) pilot study. J Am Coll Cardiol. 2011;58:1780-1791. https://doi.org/10.1016/j.jacc.2011.06.054
  7. Lim CL, Mackinnon LT. The roles of exercise-induced immune system disturbances in the pathology of heat stroke: the dual pathway model of heat stroke. Sports Med. 2006;36:39-64. https://doi.org/10.2165/00007256-200636010-00004
  8. Cook BJ, Hausenblas HA. The role of exercise dependence for the relationship between exercise behavior and eating pathology: mediator or moderator? J Health Psychol. 2008;13:495-502.
  9. Sprangers RL, Wesseling KH, Imholz AL, Imholz BP, Wieling W. Initial blood pressure fall on stand up and exercise explained by changes in total peripheral resistance. J Appl Physiol (1985). 1991;70:523-530. https://doi.org/10.1152/jappl.1991.70.2.523
  10. Ubeda Tikkanen A, Opotowsky AR, Bhatt AB, Landzberg MJ, Rhodes J. Physical activity is associated with improved aerobic exercise capacity over time in adults with congenital heart disease. Int J Cardiol. 2013;168:4685-4691. https://doi.org/10.1016/j.ijcard.2013.07.177
  11. Seo DY, Lee SR, Kim N, Ko KS, Rhee BD, Han J. Humanized animal exercise model for clinical implication. Pflugers Arch. 2014; 466:1673-1687. https://doi.org/10.1007/s00424-014-1496-0
  12. Itoh Y, Hayakawa K, Mori T, Agata N, Inoue-Miyazu M, Murakami T, Sokabe M, Kawakami K. Stand-up exercise training facilitates muscle recovery from disuse atrophy by stimulating myogenic satellite cell proliferation in mice. Physiol Rep. 2014. doi: 10.14814/phy2.12185.
  13. Helmreich DL, Parfitt DB, Lu XY, Akil H, Watson SJ. Relation between the hypothalamic-pituitary-thyroid (HPT) axis and the hypothalamic-pituitary-adrenal (HPA) axis during repeated stress. Neuroendocrinology. 2005;81:183-192. https://doi.org/10.1159/000087001
  14. Seo DY, McGregor RA, Noh SJ, Choi SJ, Mishchenko NP, Fedoreyev SA, Stonik VA, Han J. Echinochrome A improves exercise capacity during short-term endurance training in rats. Mar Drugs. 2015;13: 5722-5731. https://doi.org/10.3390/md13095722
  15. Jeffery NS, Stephenson RS, Gallagher JA, Jarvis JC, Cox PG. Micro-computed tomography with iodine staining resolves the arrangement of muscle fibres. J Biomech. 2011;44:189-192. https://doi.org/10.1016/j.jbiomech.2010.08.027
  16. Bruns RF, Menegatti CM, Martins WP, Araujo Junior E. Applicability of pocket ultrasound during the first trimester of pregnancy. Med Ultrason. 2015;17:284-288. https://doi.org/10.11152/mu.2013.2066.173.rfb
  17. Fukada S, Morikawa D, Yamamoto Y, Yoshida T, Sumie N, Yamaguchi M, Ito T, Miyagoe-Suzuki Y, Takeda S, Tsujikawa K, Yamamoto H. Genetic background affects properties of satellite cells and mdx phenotypes. Am J Pathol. 2010;176:2414-2424. https://doi.org/10.2353/ajpath.2010.090887
  18. Brenner DA, Apstein CS, Saupe KW. Exercise training attenuates age-associated diastolic dysfunction in rats. Circulation. 2001;104: 221-226. https://doi.org/10.1161/01.CIR.104.2.221
  19. Reznik M. Electron microscopy and histochemistry of regenerating skeletal muscle. Neurol India. 1972;20:71-74.
  20. Ahmet I, Tae HJ, de Cabo R, Lakatta EG, Talan MI. Effects of calorie restriction on cardioprotection and cardiovascular health. J Mol Cell Cardiol. 2011;51:263-271. https://doi.org/10.1016/j.yjmcc.2011.04.015
  21. Zhang B, Wang B, Cao S, Wang Y. Epigallocatechin-3-Gallate (EGCG) attenuates traumatic brain injury by inhibition of edema formation and oxidative stress. Korean J Physiol Pharmacol. 2015;19:491-497. https://doi.org/10.4196/kjpp.2015.19.6.491
  22. Andersson C, Lyass A, Larson MG, Spartano NL, Vita JA, Benjamin EJ, Murabito JM, Esliger DW, Blease SJ, Hamburg NM, Mitchell GF, Vasan RS. Physical activity measured by accelerometry and its associations with cardiac structure and vascular function in young and middle-aged adults. J Am Heart Assoc. 2015;4:e001528. https://doi.org/10.1161/JAHA.114.001528
  23. Harris L, Hankey C, Murray H, Melville C. The effects of physical activity interventions on preventing weight gain and the effects on body composition in young adults with intellectual disabilities: systematic review and meta-analysis of randomized controlled trials. Clin Obes. 2015;5:198-210. https://doi.org/10.1111/cob.12103
  24. Chen N, Li Q, Liu J, Jia S. Irisin, an exercise-induced myokine as a metabolic regulator: an updated narrative review. Diabetes Metab Res Rev. 2016;32:51-59. https://doi.org/10.1002/dmrr.2660
  25. Mifune H, Tajiri Y, Nishi Y, Hara K, Iwata S, Tokubuchi I, Mitsuzono R, Yamada K, Kojima M. Voluntary exercise contributed to an amelioration of abnormal feeding behavior, locomotor activity and ghrelin production concomitantly with a weight reduction in high fat diet-induced obese rats. Peptides. 2015;71:49-55. https://doi.org/10.1016/j.peptides.2015.06.007
  26. Mahajan RD, Patra SK. Irisin, a novel myokine responsible for exercise induced browning of white adipose tissue. Indian J Clin Biochem. 2013;28:102-103. https://doi.org/10.1007/s12291-012-0255-2
  27. Bluher S, Panagiotou G, Petroff D, Markert J, Wagner A, Klemm T, Filippaios A, Keller A, Mantzoros CS. Effects of a 1-year exercise and lifestyle intervention on irisin, adipokines, and inflammatory markers in obese children. Obesity (Silver Spring). 2014;22:1701-1708. https://doi.org/10.1002/oby.20739
  28. Bostrom P, Wu J, Jedrychowski MP, Korde A, Ye L, Lo JC, Rasbach KA, Bostrom EA, Choi JH, Long JZ, Kajimura S, Zingaretti MC, Vind BF, Tu H, Cinti S, Hojlund K, Gygi SP, Spiegelman BM. A PGC1-alpha-dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Nature. 2012;481:463-468. https://doi.org/10.1038/nature10777
  29. Al-Daghri NM, Alokail MS, Rahman S, Amer OE, Al-Attas OS, Alfawaz H, Tripathi G, Sabico S, Chrousos GP, McTernan PG, Piya MK. Habitual physical activity is associated with circulating irisin in healthy controls but not in subjects with diabetes mellitus type 2. Eur J Clin Invest. 2015;45:775-781. https://doi.org/10.1111/eci.12468
  30. Hew-Butler T, Landis-Piwowar K, Byrd G, Seimer M, Seigneurie N, Byrd B, Muzik O. Plasma irisin in runners and nonrunners: no favorable metabolic associations in humans. Physiol Rep. 2015. doi:10.14814/phy2.12262.
  31. Hecksteden A, Wegmann M, Steffen A, Kraushaar J, Morsch A, Ruppenthal S, Kaestner L, Meyer T. Irisin and exercise training in humans results from a randomized controlled training trial. BMC Med. 2013;11:235. https://doi.org/10.1186/1741-7015-11-235
  32. Gomez-Merino D, Drogou C, Guezennec CY, Chennaoui M. Effects of chronic exercise on cytokine production in white adipose tissue and skeletal muscle of rats. Cytokine. 2007;40:23-29. https://doi.org/10.1016/j.cyto.2007.07.188
  33. Yang X, Zhou Y, Wang P, He C, He H. Effects of whole body vibration on pulmonary function, functional exercise capacity and quality of life in people with chronic obstructive pulmonary disease: A systematic review. Clin Rehabil. 2015. doi: 10.1177/02692155155-89202. [Epub ahead of print]
  34. Nishikawa M, Ishimori N, Takada S, Saito A, Kadoguchi T, Furihata T, Fukushima A, Matsushima S, Yokota T, Kinugawa S, Tsutsui H. AST-120 ameliorates lowered exercise capacity and mitochondrial biogenesis in the skeletal muscle from mice with chronic kidney disease via reducing oxidative stress. Nephrol Dial Transplant. 2015; 30:934-942. https://doi.org/10.1093/ndt/gfv103

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