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Risk factors for the development of oral bacteria in workers according to oral environment

근로자의 구강환경요인에 따른 구강세균 발생의 위험요인

  • Hong, Min-Hee (Department of Dental Hygiene, Baekseok University)
  • 홍민희 (백석대학교 치위생학과)
  • Received : 2016.03.31
  • Accepted : 2016.06.02
  • Published : 2016.06.30

Abstract

This research examined the oral environmental factors to identify the risk factors for oral bacteria detection. This study comprised of 60 office workers aged between 20 and 65 years, and was performed from January 15 to February 28, 2015. The study variables measured were the stimulated and unstimulated salivary flow rates, salivary buffering, saliva pH, dry mouth at the dorsum of the tongue and the sublingual region, halitosis, and the degree of tongue-coating as oral environmental factors. To identify the presence of oral bacteria, pathogens were detected by extracting the gDNA of the resting salivary flow rate. The risk of S.mutans detection was 15 times higher with smokers, 1.3~1.6 times higher when the resting or stimulated salivary flow rate was reduced by 1 mm. The risk of P.intermedia detection was 13 times higher in smokers, 4.3 times higher as the severity of oral dryness was lowered, and 4 times higher for adults with a tongue coating than those without. In addition, the risk of detecting TM7 was 5.5 times higher as sublingual dryness was decreased by 1mm. The oral bacterial count will be reduced considerably by smoking cessation education and habits that facilitate a salivary flow rate. Furthermore, adults with good and well-managed dental hygiene are anticipated to have less oral bacteria and fewer dental diseases.

본 연구는 구강환경요인을 살펴보고, 구강환경이 구강세균 검출에 미치는 위험요인 알아보고자 시행하였다. 연구기간은 2015년 2월 15일~ 2월 28일까지 성인 근로자 60명을 대상으로 조사하였다. 연구변수로는 안정시, 자극시 타액분비율, 타액완충능, 타액 pH, 설배와 설하 구강건조도, 구취 설태량을 측정하였다. 구강세균의 검출 유무를 확인하기 위하여 안정시 타액분비율의 gDNA를 추출하여 균을 검출하였다. 그 결과, S.mutans균은 흡연자 15배, 자극시, 안정시 타액분비율 1.3~1.6배 위험도가 더 높았으며, P.intermedia는 흡연자 13배, 설하 구강건조도 4.3배, 설태량 4배 TM7은 설하 구강건조도 5.5배 더 높은 위험도를 나타냈다. 구강 내 세균을 줄이기 위해서는 타액분비율을 촉진시키는 습관을 형성하고, 금연교육을 시행함이 중요하다고 본다. 또한 구강위생이 깨끗하고 관리가 잘되는 성인에서는 구강세균이 많이 줄어들고, 구강질환을 좀 더 예방할 수 있을 것이라 기대된다.

Keywords

References

  1. M. D. Han, Y. K. Kim, Oral microbiology. Seoul: Komoonsa. 4nd. p.257:267:268:300:342. 2010.
  2. L. B. Parahitiyawa, C. Scully, W. K. Leung, L. J. Jin, L. Samaranayake, "Exploring the oral bacterial flora", Oral Dis, 16(2), pp.136-45, 2010. DOI: http://dx.doi.org/10.1111/j.1601-0825.2009.01607.x
  3. J. H. Badger, P. C. Ng, J. C. Venter, "The human genome, microbiomes, and disease", Metagenomics of the human body, 17(11), pp.1-14. 2011. DOI: http://dx.doi.org/10.1007/978-1-4419-7089-31
  4. S. Filoche, L. Wong, C. H. Sissons, "Oral biofilms: emerging concepts in microbial ecology", J Dent Res, 89(1), pp.8-18, 2010. DOI: http://dx.doi.org/10.1177/0022034509351812
  5. P. C. Baehni, B. Guggenheim, "Potential of diagnostic microbiology for treatment and prognosis of dental caries and periodontal diseases", Oral Biol Med, 7(3), pp.259-277, 1996. DOI: http://dx.doi.org/10.1177/10454411960070030401
  6. K. H. Kang, Y. K. Kim, H. S. Lee, J. Ingnyol, "Analysis of gene expression in response to acid stress of Streptococcus mutans isolated from a Korean child", J Korea Academia-Industrial, 10(10), pp.2990-2996, 2009. DOI: http://dx.doi.org/10.5762/KAIS.2009.10.10.2990
  7. M. M. Brinig, P. W. Lepp, C. C. Ouverney, G. C. Armitage, D. A. Relman. "Prevalence of bacteria of division TM7 in human subgingival plaque and their association with disease", Appl. Environ. Microbiol, 69(3), pp.1687-1694, 2003. DOI: http://dx.doi.org/10.1128/AEM.69.3.1687-1694.2003
  8. P. Kumar, A. Griffen, J. Barton, B. Paster, M. Moeschberger, E. Leys, "New bacterial species associated with chronic periodontitis", J Dent Res, 82(5), pp.338-344, 2003. DOI: http://dx.doi.org/10.1177/154405910308200503
  9. T. Kuehbacher, A. Rehman, P. Lepage, S. Hellmig, U. R. Folsch, S. Schreiber, S. J. Ott "Intestinal TM7 bacterial phylogenies in active inflammatory bowel disease", J Med Microbiol, 57(12), pp.1569-1576, 2008. DOI: http://dx.doi.org/10.1099/jmm.0.47719-0
  10. G. Roeselers, A. M. Guss, I. L. G. Newton, V. Klepac-Ceraj, S. Lory, C. M. Cavanaugh. "Phylogenetic and metabolic diversity of bacteria associated with cystic fibrosis", ISMEJ, 1(5), pp.20-29, 2010. DOI: http://dx.doi.org/10.1038/ismej.2010.88
  11. D. N .Fredricks, T. L. Fiedler, K. K. Thomas, B. B. Oakley, J. M. Marrazzo.. "Targeted PCR for detection of vaginal bacteria associated with bacterial vaginosis". J Clin Microbiol, 45(10), pp.3270-3276, 2007. DOI: http://dx.doi.org/10.1128/JCM.01272-07
  12. M. Feres, S. C. Cortelli, L. C. Figueiredo. "Microbiological basis for periodontal therapy: Bases microbiologicas para a terapia periodontal", J Appl. Oral Sci, 12(4), pp.256-66, 2004. DOI: http://dx.doi.org/10.1590/S1678-77572004000400002
  13. J. H. Lee. Dental biofilm, Biochem Mol Biol News (webzine) 6: 1-5, 2008. http://www.ksbmb.or.kr/sub/popup_letter.php?CatNo=653.
  14. H. K. Son, J. Y. Kim, J. R. Park, J. Kim. "The Impact of Smoking in Detection of Bacteria Related to Oral Disease and Human Papillomavirus". Kor J Oral Maxillofac Pathol. 37(6), pp.303-310, 2013.
  15. M. H. Hong, "Study on Detection of Oral Bacteria in the Saliva and Risk Factors of Adults" Korea Academia-Industrial cooperation Society, 15(9), pp.5675-5682, 2014, DOI: http://dx.doi.org/10.5762/KAIS.2014.15.9.5675
  16. M. Seki, F. Karakamaa, T. Terajimaa, Y. Ichikawaa, T. Ozakib, S. Yoshidab, Y. Yamashitaa. "Evaluation of mutans streptococci in plaque and saliva: correlation with caries development in preschool children", J Dent, 31(4), pp.283-290, 2003. DOI: http://dx.doi.org/10.1016/S0300-5712(03)00033-2
  17. H. J. Jeong, S. J. Kim. "Distribution and Antimicrobial Susceptibility of Bacteria in the Oral Cavity of Smokers or Non-Smokers", The Korean Journal of Microbiology, 46(4), pp.334-340, 2010.
  18. S. H. Son, D. A. Kim, Y. M. Park, "A Study on Dental Caries Activity Assessment from Saliva of Students of Dentistry College (I)", J Dent Hyg Sci, 13(2), pp.182-190, 2013.
  19. S. C. Cortelli, F. O. Costa, E. Rodrigues, L. T. Cota, J. R. Cortelli. "Periodontal Therapy Effects on Nitrite Related to Oral Bacteria: A 6-Month Randomized Clinical Trial.", J Periodontol, 26, pp.1-18, 2015. DOI: http://dx.doi.org/10.1902/jop.2015.140678
  20. J. O. Boyle, Z. H. Gumus, A. Kacker, V. L. Choksi, J. M.Bocker, X. K. Zhou, R. K. Yantiss, D. B. Hughes, B. Du, B. L. Judson, K. Subbaramaiah, A. J. Dannenberg. "Effects of Cigarette Smoke on the Human Oral Mucosal Transcriptome", Cancer Prev Res, 3, pp.266-278, 2010. DOI: http://dx.doi.org/10.1158/1940-6207
  21. R. C. Page, J. D. Beck. "Risk assessment for periodontal diseases", Int Dent J, 47(2), pp.61-87, 1997. DOI: http://dx.doi.org/10.1111/j.1875-595X.1997.tb00680
  22. A. D. Haffajee, S. S. Socransky, "Relationship of cigarette smoking to subgingival microbiota", J Clin Periodontol, 28(5), pp.377-388, 2001. DOI: http://dx.doi.org/10.1034/j.1600-051x.2001.028005377.x
  23. J. W. Lee, M. S. Kim, Y. H. Choi, Y. S. Chang, S. C. Shin. "A study on the relation of the stimulated salivary flow rate, pH and the viscosity", International Journal of Clinical Preventive Dentistry, 4(1), pp.112-22, 2008.
  24. W. Y. Shin. The co-relations between the various factors related with the saliva. Master's thesis, Dankook University, 2010.
  25. H. J. Choi, J. H. Kim, D. W. Lee, Y. M. Yang, JG. Kim. "Prevalence of periodontpathogens in saliva and plaque of Korean Children and Adilescents". K Korean Acad Pediatr Dent 43(1), pp.8-17. 2016. DOI: http://dx.doi.org/10.5933/JKAPD.2016.43.1.8
  26. M. A. Nadkarni, K. L. Chhour, N Hunter et al. "Age-dependent changes in porphyromonas gingivalis and prevotella specied/phylotypes in healthy gingiva and inflamed/diseased sub-gingival site". Clin Oral Investig. 19, pp.911-919, 2015. DOI: http://dx.doi.org/10.1007/s00784-014-1301-7
  27. A. A. Bosy. "Relationship of oral malodor to perodontits". J periodontol 65, pp.37-46, 1994. DOI: http://dx.doi.org/10.1902/jop.1994.65.1.37
  28. L. Xiaojing, K. M. .Kolltveit, L. Tronstad, O. Ingar. "Systemic Diseases Caused by Oral Infection" Clin Microbiol Rev, 13(4), pp.547-88, 2000. DOI: http://dx.doi.org/10.1128/CMR.13.4.547-558.2000
  29. M. Sharma, S. C. Tiwari, K. Singh, K. Kishor. "Occurrence of bacterial flora in oral infections of diabetic and non-diabetic patients", Life Sci Med Res, 32, pp.1-6, 2011. DOI: http://dx.doi.org/agris.fao.org/aos/records/DJ201209296
  30. J. L. Ebersole, S. C. Holt, R. Hansard, M. L. Novak. "Microbiologic and immunologic characteristics of periodontal disease in Hispanic americans with type 2 diabetes", J Periodontol, 79(4), pp.637-646, 2008. DOI: http://dx.doi.org/10.1902/jop.2008.070455
  31. K. E. Kempsell, C. J. Cox, M. Hurle, A. Wong, E. D. Wilkie, J. S. Zanders, H. Gaston, J. S. Crowe, "Reverse transcriptase-PCR analysis of bacterial rRNA for detection and characterization of bacterial species in arthritis synovial tissue", Infect Immun, 68(10), pp.6012-6026, 2000. DOI: http://dx.doi.org/10.1128/IAI.68.10.6012-6026.2000
  32. K. Karnoutsos, P. Papastergiou, S. Stefanidis, A. Vakaloudi, "Periodontitis as a risk factor for cardiovascular disease: The role of antiphosphorylcholine and anti-cardiolipin antibodies", HIPPOKRATIA, 12(3), pp.144-149, 2008.
  33. F. A. Scannapieco, R. B. Bush, S. Paju. "Associations between periodontal disease and risk for nosocomial bacterial pneumonia and chronic obstructive pulmonary disease. A systematic review", Ann Periodontol. 8(1). pp.54-69, 2003. DOI: http://dx.doi.org/10.1902/annals.2003.8.1.54.