The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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A Biological Activity of Serratia marcescens Strains Isolated from Dead Larva of the Diamondback Moth, Plutella xylostella (Plutellidae, Lepidoptera)

배추좀나방(Plutella xylostella)의 죽은 유충에서 분리한 Serratia marcescens 균주의 생물활성

  • Jun, Jun Hack (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Jin, Na Young (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Lee, You Kyoung (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Lee, Bo Ram (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Youn, Young Nam (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Yu, Yong Man (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
  • 전준학 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 진나영 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 이유경 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 이보람 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 윤영남 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 유용만 (충남대학교 농업생명과학대학 응용생물학과)
  • Received : 2016.05.02
  • Accepted : 2016.06.17
  • Published : 2016.06.30
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Abstract

The cause of death was investigated with several dead cabbage moth larvae in breeding box. Bacterial strains were isolated and selected from the dead larvae by bioassay. One of them was identified as Serratia marcescens used by morphological characteristics and gene sequencing. S. marcescens were cultured by Luria Bertani (LB) media broth for bioassay. When 100-fold dilution of culture broth to third larvae of diamondback moth, Plutella xylostella, it was showed a 100% mortality at 2 days after treatment, and only 10-fold dilution of supernatant liquid was showed 86.6% mortality. When the culture broth of S. marcescens was applied to the larvae of beet armyworm, Spodoptera exigua, contact and feeding toxicity were 20 and 8% of mortality, respectively. Otherwise, when the culture broth of S. marcescens was applied to 5 major plant pathogens, antibacterial activities against Fusarium oxysporum, Rhizoctonia solani, Colletotrichum gloeosporioides, Phytophthora capsici and Sclerotinias clerotiorum were 4.7, 11.3, 20, 15.7 and 42.6%, respectively. Also, degradation ability of S. marcescens against protein and chitin were examined.

배추좀나방의 죽은 유충을 사육상자에서 발견하여 죽은 원인을 조사 하였다. 죽은 유충으로부터 세균을 분리하여 생물검정으로 균주를 선발하였다. 선발된 세균의 형태 및 유전자 염기서열을 분석한 결과, Serratia marcescens로 동정되었다. 생물검정을 위하여 S. marcescens균주를 LB배양액으로 배양하여 증식하였다. 배양액을 100배로 희석하였을 때, 배추좀나방 3령 유충에 처리한 결과, 처리 2일 후에 100%의 사충률을 보였으며, 상청액만으로 처리하였을 경우에는 10배 희석액에서 86.6%의 사충률을 나타냈다. 또한 파밤나방유충에 접촉독성은 20%의 사충율을 나타냈고, 섭식독성은 8%의 효과를 보였으며, 접촉독성과 섭식독성을 동시에 수행하였을 경우에는 28%의 사충률을 보였다. 또한 국내 5개의 주요 식물병원성균에 대한 항균활성 검정에서 Fusarium oxysporum에 대해서는 4.7%, Rhizoctonia solani은 11.3%, Phytophthora capsici은 15.7%, Colletotrichum gloeosporioides은 20% 그리고 Sclerotinias clerotiorum은 42.6%의 control value를 보였다. 또한 S. marcescens 균주에 대하여 단백질과 chitin분해능력을 검토하였다.

Keywords

References

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