한국조리학회지 (Culinary science and hospitality research)

  • 제22권4호
  • /
  • Pages.128-142
  • /
  • 2016
  • /
  • 2466-0752(pISSN)
  • /
  • 2466-1023(eISSN)
한국조리학회 (Culinary Society of Korean Academy)
권호 표지

하수오 침출술이 흰쥐의 기억력 손상에 미치는 영향

Effects of Polygoni multiflori radix Liquors on the Memory Impairment of Rat

  • 공현주 (대구한의대학교 한방식품조리영양학부) ;
  • 신승렬 (대구한의대학교 한방식품조리영양학부) ;
  • 황수정 (대구한의대학교 한방식품조리영양학부) ;
  • 이경은 (영남이공대학교 간호학과) ;
  • 장정현 (부산동의과학대학교 피부미용계열) ;
  • 양경미 (대구한의대학교 한방식품조리영양학부)
  • Kong, Hyun-Joo (Faculty of Herbal Food Cuisine and Nutrition, Daegu Haany University) ;
  • Shin, Seung-Ryeul (Faculty of Herbal Food Cuisine and Nutrition, Daegu Haany University) ;
  • Hwang, Su-Jung (Faculty of Herbal Food Cuisine and Nutrition, Daegu Haany University) ;
  • Lee, Kyung Eun (School of Nursing, Yeungnam University College) ;
  • Jang, Jung-Hyeon (Division of Cosmetology, Dong-Eui Institute of Technology) ;
  • Yang, Kyung-Mi (Faculty of Herbal Food Cuisine and Nutrition, Daegu Haany University)
  • 투고 : 2016.03.22
  • 심사 : 2016.06.12
  • 발행 : 2016.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구는 하수오 침출약용주의 만성 섭취에 의한 뇌의 기억력 감퇴에 미치는 영향을 알아보고자 전체 섭취 열량의 12%를 설탕으로 공급한 정상 액체식이군, 담금 소주 액체식이군, 백하수오 침출약용주 액체식이군 및 적하수오 침출약용주 액체식이군으로 나누어 6주 동안 액체식이를 공급시킨 다음 시료를 채취하여 생화학 분석을 실시하였다. 혈청과 뇌 조직을 이용한 생화학 분석으로 알코올의 분해 능력을 알아보기 위하여 ADH와 ALDH 활성 및 acetaldehyde 농도, 그리고 뇌의 기억력 측정하기 위하여 총 choline 및 acethycholine 농도와 AChE 활성을 측정하였다. 뇌 조직에서 ADH는 정상식이군에 비해 담금 소주 알코올 섭취군에서 유의적으로 활성이 낮았으나, 담금 소주 대신에 백하수오 및 적하수오 침출 약용주를 섭취했을 때 p<0.05 수준에서 높은 활성을 보였다. ADLH 활성은 모든 군 중에서 백하수오 침출약용주를 섭취한 군에서 가장 높았으며, 알코올의 대사산물로 독성이 강한 acetaldehyde는 담금 소주 섭취군에서 높은 농도를 보였다(p<0.05). 그러나 백하수오 침출약용주를 섭취한 군에서는 낮은 acetaldehyde 농도를 보임으로써 담금 소주에 비해 백하수오 침출약용주에서 알코올 분해능력은 높고, 독성은 낮은 것으로 나타났다. 기억력 감퇴와 상관성이 높은 acetylcholine 농도는 모든 실험군 중에서 담금 소주 섭취군에서 유의적으로 가장 낮았으며(p<0.05), 이러한 결과는 담금 소주군에서 기억력의 손상을 가장 많이 받은 것으로 해석된다. 그러나 담금 소주 대신에 백하수오와 적하수오 침출약용주를 섭취한 군에서의 acetylcholine 농도는 정상식이 섭취군보다 유의적으로 더 높은 함량을 보였다(p<0.05). Acetylcholine 가수분해 효소인 혈청과 뇌 조직에서의 AChE는 정상식이군과 하수오 침출약용주에 비해 p<0.05 수분에서 높은 활성을 보였다.

This study was carried out to investigate the effect of memory impairment in rats fed alcohol liquid diets. The rats were randomly divided into 4 groups. While the control(C) group received a diet containing 12% of the daily calories with isocarbohydrate, the other groups received a diet containing 12% of the daily total calories with 36% Soju (A), 25% Cynanchi wilfordii radix liquors (WA), and 25% Polygoni multiflori radix liquors (RA) for 6 weeks. After 6 weeks, ADH activity in the brain tissue of the Group A was found to be significantly lower than Group C but significantly higher than both Groups WA and RA(p<0.05). ADLH activity was revealed to be the highest in the Group WA (p<0.05). The concentration of acetaldehyde, a highly toxic metabolite, was the highest in the Group A, but its concentration decreased significantly if fed a liquid diet containing WA. The concentration of acetylcholine, which has a high correlation with memory impairment, was significantly lower in Group A, although Group RA group was the highest compared to the other groups (p<0.05). AChE activity of the Group A was higher than Group C but lower in Groups WA and RA (p<0.05).

키워드

참고문헌

  1. Calabrese V, Renis M, Calderone A, Russo A, Reale S, Barcellona ML, Rizza V (1998). Stress proteins and SH-groups in oxidant-induced cellular injury after chronic ethanol administration in rat. Free radical Biology & Medicine 24(7/8):1159-1167. https://doi.org/10.1016/S0891-5849(97)00441-3
  2. Choi HS, Zhu MF, Kim CS, Lee JH (2003). Studies of name and herbal origins of Ha-Soo-Oh. Korean J Oriental Medicine 9(1):81-89.
  3. Choi MR, Lee MY, Hong JE, Lee JY, Chun JW, Kim TH, Shin HK, Kim EJ (2012). The aqueous extract of Rubus coreanus Miquel improve scopolamine-induced memory impairment in ICR mice. J Korean Soc Food Sci Nutr 41(2):192-196. https://doi.org/10.3746/jkfn.2012.41.2.192
  4. Ding J, Strachan MWJ, Reynolds RM, Frier BM, Deary IJ, Fowkes FGR, Lee AJ, McKnight J, Halpin P, Swa K, Price JF (2010) Diabetic retinopathy and cognitive decline in older people with type 2 diabetes. The Edinburgh type 2 diabetes study. Diabetes 59(11):2883-2889. https://doi.org/10.2337/db10-0752
  5. Elaine K (1980). The cholinergic system in old age and Alzheimer's disease. J Age Ageing 9(1):1-8. https://doi.org/10.1093/ageing/9.1.1
  6. Harper C (2009). The neuropathology of alcoholrelated brain damage. Alcohol & Alcoholism 44(2):136-140. https://doi.org/10.1093/alcalc/agn102
  7. Heo JS, Kim JB, Cho SY, Sohn KH, Choi JW (2014). Low-salt Todarodes pacificus Jeotgal improves the learning and memory impairments in scopolamine-induced dementia rats. Kor J Fish Aquat Sci 47(3):195-203. https://doi.org/10.5657/KFAS.2014.0195
  8. Herrera DG, Yague AG, Johnsen-Soriano S, Bosch-Morell F, Collado-Morente L, Muriach M, Romero FJ, Garcia-Verdugo JM (2003). Selective impairment of hippocampal neurogenesis by chronic alcoholism: Protective effects of an antioxidant. Proc Natl Acad Sci 100(13):7919-7924. https://doi.org/10.1073/pnas.1230907100
  9. Jang YE, Park JH, Roh SS, Koo JS, Seo BI (2015). Effects of Polygoni multiflori radix on prevention of hyperlipidemia and liver damage induced by alcohol. Korean J Herbol 30(3):77-82.
  10. Jo GH (2008). Alcoholism, cure to medications. Korea Public Health Association 2008(60):42-43.
  11. Jo YO, Koo SJ, Choi IS, Kong YH, Choi SY (2007). Effects of Camellia sinensis extracts on the antioxidant system and alcohol down-regulation enzymes in sub-acute ethanol treated ICR mice. J Korean Soc Food Sci Nutr 36(9):1134-1139. https://doi.org/10.3746/jkfn.2007.36.9.1134
  12. Ju MS, Kim HG, Cho HJ, Sim JJ, Jeon YJ, Oh MS (2011). Effect of Chungganhaeju-hwan in ethanol-induced neuronal cell damage. Kor J Herbol 26(3):75-82. https://doi.org/10.6116/KJH.2011.26.3.075
  13. Jung BS (1991). Metabolic effects of alcohol. Korean J Food & Nutrition 4(2):207-211.
  14. Jung HK, Park CD, Hwang MH, Park SC, Kim DI, Hong JH (2008). Characteristics of alcohol metabolism of Hahyangju in rats. Korean J Food Preserv 15(1):155-160.
  15. Kaelber C (1983). Medical disorders of alcoholism: Pathogenesis and treatment. Arch Intern Med 143(3):614.
  16. Kang JI, Ding GX, Kim KH, Ko SG (2005). Effect of Polygonum multiflorum Thunb(何首烏) on serum lipid levels in high cholesterol diet induced hyperlipidemia rats. Kor J Oriental Preventive Medical Society 9(1):105-118.
  17. Kasa P, Papp H, Kasa P, Torok I (2000). Donepezil dose-depedently inhibits acethyl-cholinesterase activity in various areas and in the presynaptic cholinergic and the postsynaptic cholinoceptive enzyme-positive structures in the human and rat brain. Neuroscience 101(1):89-100. https://doi.org/10.1016/S0306-4522(00)00335-3
  18. Kim DJ (2008a). Alcoholism is a brain disease, not a habit. Korea Pub Health Assoc 2008(62):42-43.
  19. Kim DJ (2008b). Repeat this vicious cycle of stress and drinking alcohol. Korea Pub Health Assoc 2008(59):26-27.
  20. Kim IY, Lee SJ, Kim KH (2000). Effects of Poligoni multifori radix on immunosuppretion induced by methotrexate in rat. J Society Preventive Korean Medicine 4(2):152-169.
  21. Kim JH, Lee DH, Choi SY, Lee JS (2002). Characterization of physiological functionalities in Korean traditional liquors. Korean J Food Sci Technol 34(1):118-122.
  22. Kim JH, Lee SH, Kim NM, Choi SY, Yoo JY, Lee JS (2000). Manufacture and physiological functionality of Korean traditional liquor by using Dandelion (Taraxacum platycarpum). Kor J Appl Microbiol Biotechnol 28(6):367-371.
  23. Kim JM, Park JH, Kim MK, Chun HS (2008). Effects of Paecilomyces tenuipes water extract on the alcohol metabolism of rats. J Korean Soc Food Sci Nutr 37(3):396-400. https://doi.org/10.3746/jkfn.2008.37.3.396
  24. Kim KM, Jung HJ, Sung HM, Wee JH, Kim TY, Kim KM (2014). Study of the antioxidant and alcohol-degrading enzyme activities of soybean sprout sugar solutions. Korean J Food Sci Technol 46(5):581-587. https://doi.org/10.9721/KJFST.2014.46.5.581
  25. Kim OK (2009). Antioxidative effect of Polygoni radix in streptozotocin-induced diabetic rats. Kor J Pharmacogn 40(3):213-217.
  26. Kim YS, Kim JS, Choi YH, Seo JH, Lee JW, Kim SK, Kang JS, Lee HY, Cho YS, Roh SH, Jeong YC, Shim KH, Ryu SY (2004). Ameliorating effect of the root extract from Platycodon grandiflorum on the ethanol-induced cognitive impairment in mice. Kor J Pharmacogn 35(3):239-243.
  27. Kuhl DE, Koeppe RA, Minoshima S, Snyder SE, Ficaro EP, Foster NL, Frey KA and Kilbourn MR (1999). In vivo mapping of cerebral acethylcholinesterase activity in aging and Alzheimer's disease. Neurology 52(4):691-699. https://doi.org/10.1212/WNL.52.4.691
  28. Kwak BH, Lee SS, Lee SJ, Kim KH (2003). Effect of Suoyounsoodan(首烏延毒丹) on antioxidant capacity in G-galactose induced aging rats. Kor J Oriental Preventive Medical Society 7(1):29-45.
  29. Lee DH, Park WJ, Lee BC, Lee JC, Lee DH, Lee JS (2005). Manufacture and physiological functionality of Korean traditional wine by using Gugija (Lycii fructus). Korean J Food Sci Technol 37(5):789-794.
  30. Lee EN, Lee DH, Kim SB, Lee SW, Kim NM, Lee JS (2007). Effects of medicinal plants on the quality and physiological functionalities of traditional ginseng wine. J Ginseng Res 31(2):102-108. https://doi.org/10.5142/JGR.2007.31.2.102
  31. Lee JW, Lee SS, Baek JW, Lee SJ, Kim KH (2004). Effect of Hasuohwan(何首烏丸) on antixidant capacity in D-galactose induced aging rats. Kor J Oriental Preventive Medical Society 8(1):115-133.
  32. Lee KS, Kim AJ, Lee KY (2012). Increased alcohol decomposition efficacy of Hoveina dulcis extract by carbohydrate-hydrolyzing enzymes. J East Asian Dietary Life 22(4):473-479.
  33. Lee MR, Sun BS, Gu LJ, Wang CY, Fang ZM, Wang Z, Mo EK, Ly SY, Sung CK (2009). Effects of the deer antler extract on scopolamine-induced memory impairment and its related enzyme activities. J Korean Soc Food Sci Nutr 38(4):409-414. https://doi.org/10.3746/jkfn.2009.38.4.409
  34. Lee MR. Yun BS, Liu L, Zhang DL, Wang Z, Wang CL, Gu LJ, Wang CY, Mo EK, Sung CK (2010). Effect of black ginseng on memory improvement in the amnesic mice induced by scopolamine. J Ginseng Res 34(1):51-58. https://doi.org/10.5142/JGR.2010.34.1.051
  35. Lieber CS, Decarli LM (1986). The feeding of ethanol in liquid diets. Clin Experi Research 10(5):550-553. https://doi.org/10.1111/j.1530-0277.1986.tb05140.x
  36. Mattson MP, Daun W, Pedersen WA, Culmsee C (2001). Neurodegenerative disorders and ischemic brain diseases. Apoptosis 6(1):69-81. https://doi.org/10.1023/A:1009676112184
  37. Min YK, Jeong HS (1995). Manufacture of some Korean medicinal herb liquors by soaking. Korean J Food Sci Technol 27(2):210-215.
  38. Moon BS, An TW (2007). The study about anticoagulant effect of Polygoni mutiflori radix. Daejeon University Oriental Medicine Research Institute 16(2):303-310.
  39. Moss M, Burnham EL (2003). Chronic alcohol abuse, acute respiratory distress syndrome, and multiple organ dysfunction. Critical Care Medicine 31(4):207-212. https://doi.org/10.1097/01.CCM.0000057845.77458.25
  40. Olney JW, Ishimaru MJ, Bittigau P, Ikonomidou C (2000). Ethanol-induced apoptotic neurodegeneration in the developing brain. Apoptosis 5(6):515-521. https://doi.org/10.1023/A:1009685428847
  41. Park SJ, Park DS, Kim SS, He XL, Ahn JH, Yoon WB, Lee HY (2010). The effect of fermented Codonopsis lanceolata on the memory impairment of mice. J Korean Soc Food Sci Nutr 39(11):1691-1694. https://doi.org/10.3746/jkfn.2010.39.11.1691
  42. Ritter AM, Robertson CS (1994). Cerebral metabolism. J Neurosurgery Clinics N Am 5(4):633-645.
  43. Seo JS (1999). Alcohol metabolism and nutritional effects. Food Industry and Nutrition 4(1):13-19.
  44. Seo YW, Kim HH, Go H (2008). Effect of aqueous extract of Polygoni multiflori radix on hypertension and arterial contraction in animal models. Korean J Oriental Physiology & Pathology 22(3):593-599.
  45. Umulis DM, Gurmen NM, Singh P, Fogler HS (2005). A physiologically based model for ethanol and acetaldehyde metabolism in human beings. Alcohol 35(1):3-12. https://doi.org/10.1016/j.alcohol.2004.11.004
  46. Vidal F, Toda R, Gutierrez C, Broch M, Fernadez-Muixi F, Lorenzo A, Richart C (1998). Influence of chronic alcohol abuse and liver disease on hepatic aldehyde dehydrogenase activity. Alcohol 15(1):3-8. https://doi.org/10.1016/S0741-8329(97)00073-6
  47. Won YH (2009). Alcohol in the world, smartness, taste. Daewangsa, 13-15, Seoul.