Asian-Australasian Journal of Animal Sciences

  • 제30권4호
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  • Pages.538-545
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  • 2017
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Effects of graded levels of cupric citrate on growth performance, antioxidant status, serum lipid metabolites and immunity, and tissue residues of trace elements in weaned pigs

  • Peng, Chu Cai (State Key Laboratory of Animal Nutrition, China Agricultural University) ;
  • Yan, Jia You (Institute of Animal Nutrition, Sichuan Academy of Animal Science) ;
  • Dong, Bin (State Key Laboratory of Animal Nutrition, China Agricultural University) ;
  • Zhu, Lin (State Key Laboratory of Animal Nutrition, China Agricultural University) ;
  • Tian, Yao Yao (State Key Laboratory of Animal Nutrition, China Agricultural University) ;
  • Gong, Li Min (State Key Laboratory of Animal Nutrition, China Agricultural University)
  • 투고 : 2016.03.02
  • 심사 : 2016.06.28
  • 발행 : 2017.04.01
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초록

Objective: The goal of this study was to investigate the effects of cupric citrate (CuCit) on growth performance, antioxidant indices, serum lipid metabolites, serum immune indices, and tissue residues of copper (Cu), zinc, and iron in weaned pigs. Methods: A total of 180 weaned pigs ($Duroc{\times}Landrace{\times}Large$ White) with an average body weight of $8.98{\pm}1.21kg$ were randomly assigned to a corn-soybean meal control ration, or 4 similar rations with 30, 60, 120, or 240 mg/kg Cu as CuCit. All diets contained 10 mg/kg Cu as cupric sulfate from the vitamin-mineral premix. The experiment was divided into two phases: 0 to 14 d (phase 1) and 15 to 28 d (phase 2). Results: Average daily gain (ADG; linearly, p<0.01) and average daily feed intake (ADFI; linearly and quadratically, p<0.05) were affected by an increase in CuCit during phase 2. Overall period, ADG (p<0.05) and ADFI (p<0.01) were linearly increased with increasing dietary levels of CuCit. Serum malondialdehyde concentrations (p<0.05) and glutathione peroxidase activity (p<0.01) linearly decreased and increased respectively with an increase in CuCit. Serum levels of Cu-Zn superoxide dismutase were linearly affected with an increase in CuCit (p<0.01). Hepatic malondialdehyde levels decreased with an increase in CuCit (linearly and quadratically, p<0.01). Serum total cholesterol concentrations were quadratically affected (p<0.05) and decreased in pigs fed Cu as CuCit at 60 and 120 mg/kg and increased in pigs fed 240 mg/kg Cu as CuCit. Serum high-density lipoprotein concentrations were linearly affected with an increase in CuCit (p<0.01). Serum $IL-1{\beta}$ levels were quadratically affected (p<0.05) by dietary treatment. Compared with other treatments, 240 mg/kg Cu from CuCit quadratically increased hepatic (p<0.01) and renal (p<0.05) Cu concentrations, and quadratically decreased hepatic and renal iron concentrations (p<0.05). Conclusion: Cu administered in the form of CuCit at a dosage range of 30 to 60 mg/kg, effectively enhanced the growth performance and antioxidant status of weaned pigs.

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참고문헌

  1. Committee on Nutrient Requirements of Swine, National Research Council. Nutrient requirements of swine. 11th ed. Washington, DC: National Academy Press; 2012.
  2. Cromwell GL, Stahly TS, Monegue HJ. Effects of source and level of copper on performance and liver copper stores in weanling pigs. J Anim Sci 1989;67:2996-3002. https://doi.org/10.2527/jas1989.67112996x
  3. Mei SF, Yu B, Ju CF, Zhu D, Chen DW. Effect of different levels of copper on growth performance and cecal ecosystem of newly weaned piglets. Ital J Anim Sci 2010;9:378-81.
  4. Coffey RD, Cromwell GL, Monegue HJ. Efficacy of a copper-lysine complex as a growth promotant for weanling pigs. J Anim Sci 1994;72:2880-6. https://doi.org/10.2527/1994.72112880x
  5. Zhao J, Allee G, Gerlemann G, et al. Effects of a chelated copper as growth promoter on performance and carcass traits in pigs. Asian-Australas J Anim Sci 2014;27:965-73. https://doi.org/10.5713/ajas.2013.13416
  6. Hellman NE, Gitlin JD. Ceruloplasmin metabolism and function. Annu Rev Nutr 2002;22:439-58. https://doi.org/10.1146/annurev.nutr.22.012502.114457
  7. Dove C. Copper citrate as a growth stimulating copper source for nursery pigs. J Anim Sci 1998;76:159.
  8. Armstrong TA, Cook DR, Ward MM, Williams CM, Spears JW. Effect of dietary copper source (cupric citrate and,cupric sulfate) and concentration on growth performance and fecal copper excretion in weanling pigs. J Anim Sci 2004;82:1234-40. https://doi.org/10.2527/2004.8241234x
  9. Bremner I. Manifestations of copper excess. Am J Clin Nutr 1998;67:1069S-73S. https://doi.org/10.1093/ajcn/67.5.1069S
  10. Harris ZL, Takahashi Y, Miyajima H, et al. Aceruloplasminemia: molecular characterization of this disorder of iron-metabolism. Proc Natl Acad Sci USA 1995;92:2539-43. https://doi.org/10.1073/pnas.92.7.2539
  11. Amer M, Elliot J. Effects of supplemental dietary copper on glyceride distribution in the backfat of pigs. Can J Anim Sci 1973;53:147-52. https://doi.org/10.4141/cjas73-022
  12. Amer M, Elliot J. Effects of level of copper supplement and removal of supplemental copper from the diet on the physical and chemical characteristics of porcine depot fat. Can J Anim Sci 1973;53:139-45. https://doi.org/10.4141/cjas73-021
  13. Massie HR, Ofosuappiah W, Aiello VR. Elevated serum copper is associated with reduced immune response in aging mice. Gerontology 1993;39:136-45. https://doi.org/10.1159/000213525
  14. Percival SS. Copper and immunity. Am J Clin Nutr 1998;67:1064S-8S. https://doi.org/10.1093/ajcn/67.5.1064S
  15. Hill G, Miller E, Whetter P, Ullrey D. Concentration of minerals in tissues of pigs from dams fed different levels of dietary zinc. J Anim Sci 1983;57:130-8. https://doi.org/10.2527/jas1983.571130x
  16. Huang YL, Ashwell MS, Fry RS, et al. Effect of dietary copper amount and source on copper metabolism and oxidative stress of weanling pigs in short-term feeding. J Anim Sci 2015;93:2948-55. https://doi.org/10.2527/jas.2014-8082
  17. Li J, Yan L, Zheng X, et al. Effect of high dietary copper on weight gain and neuropeptide Y level in the hypothalamus of pigs. J Trace Elem Med Biol 2008;22:33-8. https://doi.org/10.1016/j.jtemb.2007.10.003
  18. Zhu D, Yu B, Ju C, Mei S, Chen D. Effect of high dietary copper on the expression of hypothalamic appetite regulators in weanling pigs. J Anim Feed Sci 2011;20:60-70. https://doi.org/10.22358/jafs/66158/2011
  19. Ma YL, Zanton GI, Zhao J, et al. Multitrial analysis of the effects of copper level and source on performance in nursery pigs. J Anim Sci 2015;93:606-14. https://doi.org/10.2527/jas.2014-7796
  20. Armstrong TA, Spears JW, van Heugten E, Engle TE, Wright CL. Effect of copper source (cupric citrate vs cupric sulfate) and level on growth performance and copper metabolism in pigs. Asian-Australas J Anim Sci 2000;13:1154-61. https://doi.org/10.5713/ajas.2000.1154
  21. Kim BE, Nevitt T, Thiele DJ. Mechanisms for copper acquisition, distribution and regulation. Nat Chem Biol 2008;4:176-85. https://doi.org/10.1038/nchembio.72
  22. Pastorelli G, Rossi R, Zanardi E, Ghidini S, Corino C. Two different forms and levels of CuSO4 in piglet feeding: liver, plasma and faeces copper status. J Anim Feed Sci 2014;23:52-7. https://doi.org/10.22358/jafs/65716/2014
  23. Cater MA, La Fontaine S, Shield K, Deal Y, Mercer JFB. ATP7B mediates vesicular sequestration of copper: Insight into biliary copper excretion. Gastroenterology 2006;130:493-506. https://doi.org/10.1053/j.gastro.2005.10.054
  24. Kaim W, Rall J. Copper-a "modern" bioelement. Angew Chem Int Edit 1996;35:43-60. https://doi.org/10.1002/anie.199600431
  25. Ward JD, Spears JW. Long-term effects of consumption of low-copper diets with or without supplemental molybdenum on copper status, performance, and carcass characteristics of cattle. J Anim Sci 1997;75:3057-65. https://doi.org/10.2527/1997.75113057x
  26. Senthilkumar P, Nagalakshmi D, Reddy YR, Sudhakar K. Effect of different level and source of copper supplementation on immune response and copper dependent enzyme activity in lambs. Trop Anim Health Prod 2009;41:645-53. https://doi.org/10.1007/s11250-008-9236-0
  27. Draper H, Hadley M. Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol 1989;186:421-31.
  28. Fry RS, Ashwell MS, Lloyd KE, et al. Amount and source of dietary copper affects small intestine morphology, duodenal lipid peroxidation, hepatic oxidative stress, and mRNA expression of hepatic copper regulatory proteins in weanling pigs. J Anim Sci 2012;90:3112-9. https://doi.org/10.2527/jas.2011-4403
  29. Feng J, Ma WQ, Gu ZL, Wang YZ, Liu JX. Effects of dietary copper (II) sulfate and copper proteinate on performance and blood indexes of copper status in growing pigs. Biol Trace Elem Res 2007;120:171-8. https://doi.org/10.1007/s12011-007-8001-y
  30. Armstrong TA, Spears JW, Engle TE, See MT. Effect of pharmacological concentrations of dietary copper on lipid and cholesterol metabolism in pigs. Nutr Res 2001;21:1299-308. https://doi.org/10.1016/S0271-5317(01)00332-3
  31. Li QH, Luo X, Liu B, Han J, Yu S. The effect of dietary copper as copper-glycine on blood physiological and biochemical traits and tissue copper contents of weanling pigs. Acta Vet Zootech Sin 2004;35:23-7 (In chinese with english abstract).
  32. Bonham M, O'Connor JM, Hannigan BM, Strain J. The immune system as a physiological indicator of marginal copper status? Br J Nutr 2002;87:393-403. https://doi.org/10.1079/BJN2002558
  33. Kelley DS, Daudu PA, Taylor PC, Mackey BE, Turnlund JR. Effects of low-copper diets on human immune response. Am J Clin Nutr 1995;62:412-6. https://doi.org/10.1093/ajcn/62.2.412
  34. Wang XX, Song PX, Wu H, et al. Effects of graded levels of isomaltooligosaccharides on the performance, immune function and intestinal status of weaned pigs. Asian-Australas J Anim Sci 2016;29:250-6.
  35. Yan JY, Zhang C, Tang L, Kuang SY. Effect of dietary copper sources and concentrations on serum lysozyme concentration and protegrin-1 gene expression in weaning piglets. Ital J Anim Sci 2015;14:3709. https://doi.org/10.4081/ijas.2015.3709
  36. Espinoza A, Le Blanc S, Olivares M, et al. Iron, copper, and zinc transport: inhibition of divalent metal transporter 1 (DMT1) and human copper transporter 1 (hCTR1) by shRNA. Biol Trace Elem Res 2012;146:281-6. https://doi.org/10.1007/s12011-011-9243-2
  37. Collins JF, Prohaska JR, Knutson MD. Metabolic crossroads of iron and copper. Nutr Rev 2010;68:133-47. https://doi.org/10.1111/j.1753-4887.2010.00271.x
  38. Vasak M, Meloni G. Chemistry and biology of mammalian metallothioneins. J Biol Inorg Chem 2011;16:1067-78. https://doi.org/10.1007/s00775-011-0799-2
  39. Pang Y, Applegate TJ. Effects of dietary copper supplementation and copper source on digesta pH, calcium, zinc, and copper complex size in the gastrointestinal tract of the broiler chicken. Poult Sci 2007;86:531-7. https://doi.org/10.1093/ps/86.3.531

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