The Korean Journal of Physiology and Pharmacology

  • 제21권2호
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  • Pages.161-168
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  • 2017
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  • 1226-4512(pISSN)
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  • 2093-3827(eISSN)
대한약리학회 (The Korean Society of Pharmacology)
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Paracrine influence of human perivascular cells on the proliferation of adenocarcinoma alveolar epithelial cells

  • Kim, Eunbi (Department of Internal Medicine, School of Medicine, Kangwon National University) ;
  • Na, Sunghun (Department of Obstetrics & Gynecology, School of Medicine, Kangwon National University) ;
  • An, Borim (Department of Internal Medicine, School of Medicine, Kangwon National University) ;
  • Yang, Se-Ran (Department of Thoracic and Cardiovascular Surgery, School of Medicine, Kangwon National University) ;
  • Kim, Woo Jin (Department of Internal Medicine, School of Medicine, Kangwon National University) ;
  • Ha, Kwon-Soo (Department of Molecular and Cellular Biochemistry, School of Medicine, Kangwon National University) ;
  • Han, Eun-Taek (Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University) ;
  • Park, Won Sun (Department of Physiology, School of Medicine, Kangwon National University) ;
  • Lee, Chang-Min (Department of Molecular Microbiology and Immunology, Department of Medicine, Alpert Medical School, Brown University) ;
  • Lee, Ji Yoon (Department of Biomedical Laboratory Science, College of Health Sciences, Sanji University) ;
  • Lee, Seung-Joon (Department of Internal Medicine, School of Medicine, Kangwon National University) ;
  • Hong, Seok-Ho (Department of Internal Medicine, School of Medicine, Kangwon National University)
  • 투고 : 2016.09.07
  • 심사 : 2017.01.09
  • 발행 : 2017.03.01
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초록

Understanding the crosstalk mechanisms between perivascular cells (PVCs) and cancer cells might be beneficial in preventing cancer development and metastasis. In this study, we investigated the paracrine influence of PVCs derived from human umbilical cords on the proliferation of lung adenocarcinoma epithelial cells (A549) and erythroleukemia cells (TF-$1{\alpha}$ and K562) in vitro using $Transwell^{(R)}$ co-culture systems. PVCs promoted the proliferation of A549 cells without inducing morphological changes, but had no effect on the proliferation of TF-$1{\alpha}$ and K562 cells. To identify the factors secreted from PVCs, conditioned media harvested from PVC cultures were analyzed by antibody arrays. We identified a set of cytokines, including persephin (PSPN), a neurotrophic factor, and a key regulator of oral squamous cell carcinoma progression. Supplementation with PSPN significantly increased the proliferation of A549 cells. These results suggested that PVCs produced a differential effect on the proliferation of cancer cells in a cell-type dependent manner. Further, secretome analyses of PVCs and the elucidation of the molecular mechanisms could facilitate the discovery of therapeutic target(s) for lung cancer.

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